LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2369..2465) /label=attL2 /note="attL2 (c)" misc_signal complement(2480..2499) /note="T7 Promoter/priming site (c)" primer_bind 2507..2523 /note="M13 Reverse priming site" gene 2636..3445 /note="Kanamycin resistance gene" rep_origin 3566..4239 /note="pUC origin" vector join(2377..4242,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2368 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:71683 IMAGE:5263292" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..2368 /gene="MAN1A2" /gene_synonym=MAN1B /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" CDS 692..2368 /dnas_title="mannosidase, alpha, class 1A, member 2" /gene="MAN1A2" /gene_synonym=MAN1B /codon_start=1 /product="mannosidase, alpha, class 1A, member 2" /protein_id="AAH63300.1" /db_xref="GI:38969810" /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" /translation="MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILS AFITLCFGAFFFLPDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHRE EEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVP IPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIF GSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLL AAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILA EFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHT SVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKN GHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPE SFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCR VNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPL PVLHLANTTLSGNPAVR" misc_feature 692..2368 /note="Man1A2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgctaaaaac cccggagtct tcctgatcca 721 tggacccgat gaacatagac acagggaaga ggaagaacgt ctgagaaata aaattcgagc 781 tgatcatgag aaggccttgg aagaagcaaa agaaaaatta agaaagtcaa gagaggaaat 841 tcgagcagaa attcagacag agaaaaataa ggtagtccaa gaaatgaaga taaaagagaa 901 caagccactg ccaccagtcc ctattcccaa ccttgtagga atacgtggtg gagacccaga 961 agataatgac ataagagaga aaagggaaaa aattaaagag atgatgaaac atgcttggga 1021 taactatagg acatatgggt ggggacataa tgaactcaga cctattgcaa ggaaaggaca 1081 ctcccctaac atatttggaa gttcacaaat gggtgctacc atagtagatg ctttggatac 1141 cctttatatc atgggacttc atgatgaatt cctagatggg caaagatgga ttgaagacaa 1201 ccttgatttc agtgtgaatt cagaggtgtc tgtgtttgaa gtcaacattc gatttattgg 1261 aggcctactt gcagcatatt acctatcagg agaggagata ttcaagatta aagcagtgca 1321 attggctgag aaactccttc ctgcctttaa cacacctact gggattcctt gggcaatggt 1381 gaatttgaaa agtggagtag ggcgaaactg gggctgggca tctgcaggta gcagcattct 1441 ggctgaattt ggtacactac atatggagtt catccacctc agctacttga caggggacct 1501 gacttactac aaaaaggtta tgcacattcg gaaactactt cagaaaatgg atcgtccaaa 1561 tggtctttat ccaaattatt tgaaccccag aacagggcgc tggggtcagt atcatacatc 1621 tgtcggtggc ctgggagaca gtttttatga atacttactg aaagcatggt tgatgtcaga 1681 taaaacagac catgaggcaa gaaagatgta tgatgatgct attgaggcta tagaaaaaca 1741 tcttattaag aagtctcgtg gaggtcttac ctttattgga gaatggaaga atgggcactt 1801 ggaaaaaaag atggggcatt tggcctgctt tgctggggga atgtttgcac taggagcaga 1861 tggttccaga gcagataaag ctggtcatta tttagagcta ggggcagaaa ttgcacgtac 1921 ttgtcatgag tcatatgaca gaactgcatt aaagctaggt cctgaatcat tcaagtttga 1981 tggtgcagtg gaggctgtgg ctgtccggca ggctgaaaag tattatatcc tccgtccaga 2041 agtaattgaa acctattggt acctatggcg attcactcac gatccaagat acaggcagtg 2101 gggctgggaa gcagcactgg ccattgaaaa gtattgccga gttaatggtg ggttttctgg 2161 agtcaaagat gtatattcct ctactcctac acatgatgat gtacagcaga gcttttttct 2221 tgctgaaaca ttaaaatatt tgtatctgct gttctccggt gatgaccttt tacctttaga 2281 ccactgggtg tttaatacag aggctcaccc tctgcctgtg ttacatttag ccaacaccac 2341 actttcaggt aatcctgctg ttcgatgaCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2401 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2461 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2521 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2581 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2641 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2701 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2761 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2821 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2881 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 2941 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 3001 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 3061 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 3121 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 3181 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 3241 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 3301 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3361 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3421 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3481 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3541 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3601 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3661 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3721 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3781 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3841 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3901 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3961 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4021 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4081 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4141 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4201 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //