LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2372..2468) /label=attL2 /note="attL2 (c)" misc_signal complement(2483..2502) /note="T7 Promoter/priming site (c)" primer_bind 2510..2526 /note="M13 Reverse priming site" gene 2639..3448 /note="Kanamycin resistance gene" rep_origin 3569..4242 /note="pUC origin" vector join(2380..4245,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2371 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1215 IMAGE:3533651" /tissue_type="Lung, small cell carcinoma" /clone_lib="NIH_MGC_7" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2371 /gene="MAN1B1" /gene_synonym=MANA-ER /db_xref="GeneID:11253" /db_xref="HGNC:6823" /db_xref="MIM:604346" CDS 692..2371 /dnas_title="mannosidase, alpha, class 1B, member 1" /gene="MAN1B1" /gene_synonym=MANA-ER /codon_start=1 /product="mannosidase, alpha, class 1B, member 1" /protein_id="AAH02953.1" /db_xref="GI:12804191" /db_xref="GeneID:11253" /db_xref="HGNC:6823" /db_xref="MIM:604346" /translation="MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPP PHRDFISVTLSFGESYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYI NLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRH IQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQG TELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKP VSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIR ILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSD STVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLF THLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLT FVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETG LSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEIL QSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLD AYVFNTEAHPLPIWTPA" misc_feature 692..2371 /note="MAN1B1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaggcggac accgaccctg agaacttacc 721 tgagatttcg tcacagaaga cacaaagaca catccagcgg ggaccacctc acctgcagat 781 tagaccccca agccaagacc tgaaggatgg gacccaggag gaggccacaa aaaggcaaga 841 agcccctgtg gatccccgcc cggaaggaga tccgcagagg acagtcatca gctggagggg 901 agcggtgatc gagcctgagc agggcaccga gctcccttca agaagagcag aagtgcccac 961 caagcctccc ctgccaccgg ccaggacaca gggcacacca gtgcatctga actatcgcca 1021 gaagggcgtg attgacgtct tcctgcatgc atggaaagga taccgcaagt ttgcatgggg 1081 ccatgacgag ctgaagcctg tgtccaggtc cttcagtgag tggtttggcc tcggtctcac 1141 actgatcgac gcgctggaca ccatgtggat cttgggtctg aggaaagaat ttgaggaagc 1201 caggaagtgg gtgtcgaaga agttacactt tgaaaaggac gtggacgtca acctgtttga 1261 gagcacgatc cgcatcctgg gggggctcct gagtgcctac cacctgtctg gggacagcct 1321 cttcctgagg aaagctgagg attttggaaa tcggctaatg cctgccttca gaacaccatc 1381 caagattcct tactcggatg tgaacatcgg tactggagtt gcccacccgc cacggtggac 1441 ctccgacagc actgtggccg aggtgaccag cattcagctg gagttccggg agctctcccg 1501 tctcacaggg gataagaagt ttcaggaggc agtggagaag gtgacacagc acatccacgg 1561 cctgtctggg aagaaggatg ggctggtgcc catgttcatc aatacccaca gtggcctctt 1621 cacccacctg ggcgtattca cgctgggcgc cagggccgac agctactatg agtacctgct 1681 gaagcagtgg atccagggcg ggaagcagga gacacagctg ctggaagact acgtggaagc 1741 catcgagggt gtcagaacgc acctgctgcg gcactccgag cccagtaagc tcacctttgt 1801 gggggagctt gcccacggcc gcttcagtgc caagatggac cacctggtgt gcttcctgcc 1861 agggacgctg gctctgggcg tctaccacgg cctgcccgcc agccacatgg agctggccca 1921 ggagctcatg gagacttgtt accagatgaa ccggcagatg gagacggggc tgagtcccga 1981 gatcgtgcac ttcaaccttt acccccagcc gggccgtcgg gacgtggagg tcaagccagc 2041 agacaggcac aacctgctgc ggccagagac cgtggagagc ctgttctacc tgtaccgcgt 2101 cacaggggac cgcaaatacc aggactgggg ctgggagatt ctgcagagct tcagccgatt 2161 cacacgggtc ccctcgggtg gctattcttc catcaacaat gtccaggatc ctcagaagcc 2221 cgagcctagg gacaagatgg agagcttctt cctgggggag acgctcaagt atctgttctt 2281 gctcttctcc gatgacccaa acctgctcag cctggatgcc tacgtgttca acaccgaagc 2341 ccaccctctg cctatctgga cccctgccta gCCAGCTTTC TTGTACAAAG TTGGCATTAT 2401 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2461 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2521 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2581 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2641 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2701 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2761 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2821 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2881 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2941 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 3001 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 3061 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 3121 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 3181 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 3241 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 3301 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 3361 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3421 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3481 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3541 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3601 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3661 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3721 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3781 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3841 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3901 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3961 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 4021 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4081 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4141 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4201 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //