LOCUS MAN1B1-pGEn1-DES 6256 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4884..5425 /note="WPRE" misc_recomb 4846..4868 /label=attB2 /note="attB2 site" misc_feature complement(5432..5646) /note="BGH pA" misc_feature complement(5734..5863) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4845 /dnas_title="MAN1B1-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1215 IMAGE:3533651" /tissue_type="Lung, small cell carcinoma" /clone_lib="NIH_MGC_7" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..4845 /dnas_title="MAN1B1" /gene="MAN1B1" /gene_synonym="MANA-ER" /db_xref="GeneID:11253" /db_xref="HGNC:6823" /db_xref="MIM:604346" CDS 3166..4845 /dnas_title="mannosidase, alpha, class 1B, member 1" /gene="MAN1B1" /gene_synonym="MANA-ER" /codon_start=1 /product="mannosidase, alpha, class 1B, member 1" /protein_id="AAH02953.1" /db_xref="GI:12804191" /db_xref="GeneID:11253" /db_xref="HGNC:6823" /db_xref="MIM:604346" /translation="MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPP PHRDFISVTLSFGESYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYI NLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRH IQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQG TELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKP VSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIR ILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSD STVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLF THLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLT FVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETG LSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEIL QSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLD AYVFNTEAHPLPIWTPA" misc_feature 3166..4845 /note="MAN1B1 coding region" vector join(4854..6256,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4853 /source="MAN1B1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcaaggc ggacaccgac 3181 cctgagaact tacctgagat ttcgtcacag aagacacaaa gacacatcca gcggggacca 3241 cctcacctgc agattagacc cccaagccaa gacctgaagg atgggaccca ggaggaggcc 3301 acaaaaaggc aagaagcccc tgtggatccc cgcccggaag gagatccgca gaggacagtc 3361 atcagctgga ggggagcggt gatcgagcct gagcagggca ccgagctccc ttcaagaaga 3421 gcagaagtgc ccaccaagcc tcccctgcca ccggccagga cacagggcac accagtgcat 3481 ctgaactatc gccagaaggg cgtgattgac gtcttcctgc atgcatggaa aggataccgc 3541 aagtttgcat ggggccatga cgagctgaag cctgtgtcca ggtccttcag tgagtggttt 3601 ggcctcggtc tcacactgat cgacgcgctg gacaccatgt ggatcttggg tctgaggaaa 3661 gaatttgagg aagccaggaa gtgggtgtcg aagaagttac actttgaaaa ggacgtggac 3721 gtcaacctgt ttgagagcac gatccgcatc ctgggggggc tcctgagtgc ctaccacctg 3781 tctggggaca gcctcttcct gaggaaagct gaggattttg gaaatcggct aatgcctgcc 3841 ttcagaacac catccaagat tccttactcg gatgtgaaca tcggtactgg agttgcccac 3901 ccgccacggt ggacctccga cagcactgtg gccgaggtga ccagcattca gctggagttc 3961 cgggagctct cccgtctcac aggggataag aagtttcagg aggcagtgga gaaggtgaca 4021 cagcacatcc acggcctgtc tgggaagaag gatgggctgg tgcccatgtt catcaatacc 4081 cacagtggcc tcttcaccca cctgggcgta ttcacgctgg gcgccagggc cgacagctac 4141 tatgagtacc tgctgaagca gtggatccag ggcgggaagc aggagacaca gctgctggaa 4201 gactacgtgg aagccatcga gggtgtcaga acgcacctgc tgcggcactc cgagcccagt 4261 aagctcacct ttgtggggga gcttgcccac ggccgcttca gtgccaagat ggaccacctg 4321 gtgtgcttcc tgccagggac gctggctctg ggcgtctacc acggcctgcc cgccagccac 4381 atggagctgg cccaggagct catggagact tgttaccaga tgaaccggca gatggagacg 4441 gggctgagtc ccgagatcgt gcacttcaac ctttaccccc agccgggccg tcgggacgtg 4501 gaggtcaagc cagcagacag gcacaacctg ctgcggccag agaccgtgga gagcctgttc 4561 tacctgtacc gcgtcacagg ggaccgcaaa taccaggact ggggctggga gattctgcag 4621 agcttcagcc gattcacacg ggtcccctcg ggtggctatt cttccatcaa caatgtccag 4681 gatcctcaga agcccgagcc tagggacaag atggagagct tcttcctggg ggagacgctc 4741 aagtatctgt tcttgctctt ctccgatgac ccaaacctgc tcagcctgga tgcctacgtg 4801 ttcaacaccg aagcccaccc tctgcctatc tggacccctg cctagCCAGC TTTCTTGTAC 4861 AAAGTGGTTG ATAGCTTGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 4921 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 4981 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 5041 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 5101 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 5161 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 5221 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 5281 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 5341 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 5401 CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC 5461 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 5521 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 5581 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 5641 TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG 5701 ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA 5761 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC 5821 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA 5881 TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT 5941 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 6001 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 6061 CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG 6121 GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 6181 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 6241 AACGCTTACA ATTTAG //