LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2381..2477) /label=attL2 /note="attL2 (c)" misc_signal complement(2492..2511) /note="T7 Promoter/priming site (c)" primer_bind 2519..2535 /note="M13 Reverse priming site" gene 2648..3457 /note="Kanamycin resistance gene" rep_origin 3578..4251 /note="pUC origin" vector join(2389..4254,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2380 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:168633 IMAGE:9021010" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: GGGGGCAA" gene 692..2380 /gene="MAN1C1" /gene_synonym=HMIC /gene_synonym=MAN1A3 /gene_synonym=MAN1C /gene_synonym=pp6318 /db_xref="GeneID:57134" /db_xref="HGNC:19080" misc_feature 692..2380 /gene="MAN1C1" /gene_synonym=HMIC /gene_synonym=MAN1A3 /gene_synonym=MAN1C /gene_synonym=pp6318 /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..2380 /dnas_title="mannosidase, alpha, class 1C, member 1" /gene="MAN1C1" /gene_synonym=HMIC /gene_synonym=MAN1A3 /gene_synonym=MAN1C /gene_synonym=pp6318 /codon_start=1 /product="mannosidase, alpha, class 1C, member 1" /protein_id="AAI37018.1" /db_xref="GI:187952495" /db_xref="GeneID:57134" /db_xref="HGNC:19080" /translation="MLMRKVPGFVPASPWGLRLPQKFLFLLFLSGLVTLCFGALFLLP HSSRLKRLFLAPRTQQPGLEVVAEIAGHAPAREQEPPPNPAPAAPAPGEDDPSSWASP RRRKGGLRRTRPTGPREEATAARGNSIPASRPGDEGVPFRFDFNAFRSRLRHPVLGTR ADESQEPQSQVRAQREKIKEMMQFAWQSYKRYAMGKNELRPLTKDGYEGNMFGGLSGA TVIDSLDTLYLMELKEEFQEAKAWVGESFHLNVSGEASLFEVNIRYIGGLLSAFYLTG EEVFRIKAIRLGEKLLPAFNTPTGIPKGVVSFKSGNWGWATAGSSSILAEFGSLHLEF LHLTELSGNQVFAEKVRNIRKVLRKIEKPFGLYPNFLSPVSGNWVQHHVSVGGLGDSF YEYLIKSWLMSGKTDMEAKNMYYEALEAIETYLLNVSPGGLTYIAEWRGGILDHKMGH LACFSGGMIALGAEDAKEEKRAHYRELAAQITKTCHESYARSDTKLGPEAFWFNSGRE AVATQLSESYYILRPEVVESYMYLWRQTHNPIYREWGWEVVLALEKYCRTEAGFSGIQ DVYSSTPNHDNKQQSFFLAETLKYLYLLFSEDDLLSLEDWVFNTEAHPLPVNHSDSSG RAWGRH" misc_feature 692..2380 /note="MAN1C1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catcgccggc catgccccgg cccgcgagca 721 ggagccgcct cccaacccgg cccccgccgc gccggccccg ggcgaggatg accccagcag 781 ctgggccagt ccccgccgca ggaaaggggg gctgcggcgc acccgcccca ctggaccccg 841 cgaggaggcc acggcggccc ggggcaatag catcccggcc tccaggcccg gggacgaggg 901 cgtccctttc cgctttgact tcaacgcatt ccggagccgt ctccgccacc cggtcctggg 961 aacgagggcc gatgagagtc aggagcccca gagccaagtg cgagcccagc gggagaaaat 1021 caaggagatg atgcagtttg cttggcagag ctataagcgt tatgcaatgg ggaaaaacga 1081 actccgtcca ctaacaaaag atggctacga gggtaacatg ttcggaggcc tcagcggggc 1141 aacagtcatt gactccctcg ataccctcta cctcatggag ctgaaggagg agttccagga 1201 ggccaaggcc tgggtgggag agagcttcca cctgaacgtg agcggagaag catccttgtt 1261 tgaggtgaac atccgctaca tcgggggact cctctcagcc ttctacctga caggagaaga 1321 ggtgttccga ataaaggcca tcaggctggg agagaagctc ctgccggcgt tcaacacccc 1381 cacgggaatc ccaaagggcg tggtgagctt caaaagtggg aactggggct gggccacagc 1441 cggcagcagc agcatcttgg cggagtttgg atccctgcac ttggaattct tacacctcac 1501 tgaactctct ggcaaccagg tcttcgctga aaaggtcagg aacatccgca aggtcctcag 1561 gaagatcgaa aagccctttg gcctctaccc caacttcctc agcccagtga gtgggaactg 1621 ggtgcaacac catgtctcag ttggaggact cggggacagt ttttatgaat atttgatcaa 1681 atcctggttg atgtcgggca agacagatat ggaggctaaa aatatgtact acgaagcctt 1741 ggaggcgata gagacctact tgctgaatgt ctctcccggg gggctgacct acattgccga 1801 gtggcgaggg gggattctgg accacaagat ggggcacctg gcctgtttct ccgggggcat 1861 gatcgccctt ggcgccgagg atgccaagga agaaaagagg gcccactacc gagagctcgc 1921 agcccagatc accaagacgt gtcacgagtc atacgcccgc tcagacacca aacttgggcc 1981 tgaggccttc tggtttaact ccggcagaga ggccgtggcc acccagctga gcgagagcta 2041 ctacatcctc cggccagagg tggtggagag ctacatgtac ctgtggcgac agacccacaa 2101 ccccatctac agggagtggg gctgggaggt ggtgctggcc ttggagaaat actgtcggac 2161 agaagccggt ttctctggga tccaagacgt gtacagtagc acccccaacc acgacaacaa 2221 gcagcagagc ttctttctag cggagacact aaagtatctc tatcttctgt tctctgaaga 2281 tgacttgctc tccctggaag actgggtgtt caacaccgag gcccacccac tcccggtgaa 2341 ccactcagac agctccggca gagcctgggg cagacactga CCAGCTTTCT TGTACAAAGT 2401 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2461 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2521 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 2581 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2641 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2701 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2761 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2821 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2881 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2941 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 3001 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 3061 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 3121 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 3181 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 3241 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 3301 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 3361 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 3421 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 3481 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3541 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3601 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3661 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3721 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3781 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3841 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3901 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3961 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 4021 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 4081 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 4141 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 4201 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //