LOCUS HYAL1-pGEc2-DEST 6526 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6004..6133) /note="3'-ITR" misc_feature complement(5702..5916) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5154..5695 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4333..4355 /note="attB2" misc_feature 5133..5138 /note="BstB1 site" misc_feature 4356..5051 /note="Super GFP" misc_feature 5052..5102 /note="AviTag" misc_feature 5103..5126 /note="His Tag" PCR_frame 3002..4333 /note="Reading Frame" /set="Set 1" source 3006..4310 /dnas_title="HYAL1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:45987 IMAGE:5760636" /tissue_type="Colon, Kidney, Stomach, adult, whole pooled" /clone_lib="NIH_MGC_116" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4310 /dnas_title="HYAL1" /gene="HYAL1" /gene_synonym="HYAL-1" /gene_synonym="LUCA1" /gene_synonym="MGC45987" /gene_synonym="NAT6" /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" CDS 3006..4310 /dnas_title="hyaluronoglucosaminidase 1" /gene="HYAL1" /gene_synonym="HYAL-1" /gene_synonym="LUCA1" /gene_synonym="MGC45987" /gene_synonym="NAT6" /codon_start=1 /product="hyaluronoglucosaminidase 1" /protein_id="AAH35695.1" /db_xref="GI:23273469" /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" /translation="MAAHLLPICALFLTLLDMAQGFRGPLLPNRPFTTVWNANTQWCL ERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASL IAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHP DWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYT GQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAA GDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQ AIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLT PGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW" misc_feature 3006..4310 /note="HYAL1 coding region" misc_feature 4311..4331 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4340) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4340) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4341..6526,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4340 /source="HYAL1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc agcccacctg cttcccatct gcgccctctt cctgacctta ctcgatatgg 3061 cccaaggctt taggggcccc ttgctaccca accggccctt caccaccgtc tggaatgcaa 3121 acacccagtg gtgcctggag aggcacggtg tggacgtgga tgtcagtgtc ttcgatgtgg 3181 tagccaaccc agggcagacc ttccgcggcc ctgacatgac aattttctat agctcccagc 3241 tgggcaccta cccctactac acgcccactg gggagcctgt gtttggtggt ctgccccaga 3301 atgccagcct gattgcccac ctggcccgca cattccagga catcctggct gccatacctg 3361 ctcctgactt ctcagggctg gcagtcatcg actgggaggc atggcgccca cgctgggcct 3421 tcaactggga caccaaggac atttaccggc agcgctcacg ggcactggta caggcacagc 3481 accctgattg gccagctcct caggtggagg cagtagccca ggaccagttc cagggagctg 3541 cacgggcctg gatggcaggc accctccagc tggggcgggc actgcgtcct cgcggcctct 3601 ggggcttcta tggcttccct gactgctaca actatgactt tctaagcccc aactacaccg 3661 gccagtgccc atcaggcatc cgtgcccaaa atgaccagct agggtggctg tggggccaga 3721 gccgtgccct ctatcccagc atctacatgc ccgcagtgct ggagggcaca gggaagtcac 3781 agatgtatgt gcaacaccgt gtggccgagg cattccgtgt ggctgtggct gctggtgacc 3841 ccaatctgcc ggtgctgccc tatgtccaga tcttctatga cacgacaaac cactttctgc 3901 ccctggatga gctggagcac agcctggggg agagtgcggc ccagggggca gctggagtgg 3961 tgctctgggt gagctgggaa aatacaagaa ccaaggaatc atgtcaggcc atcaaggagt 4021 atatggacac tacactgggg cccttcatcc tgaacgtgac cagtggggcc cttctctgca 4081 gtcaagccct gtgctccggc catggccgct gtgtccgccg caccagccac cccaaagccc 4141 tcctcctcct taaccctgcc agtttctcca tccagctcac gcctggtggt gggcccctga 4201 gcctgcgggg tgccctctca cttgaagatc aggcacagat ggctgtggag ttcaaatgtc 4261 gatgctaccc tggctggcag gcaccgtggt gtgagcggaa gagcatgtgg gaaaacttgt 4321 actttcaagg ctCCAGCTTT CTTGTACAAA GTGGTATGAG CAAGGGCGAG GAACTGTTCA 4381 CCGGCGTGGT GCCCATCCTG GTGGAACTGG ATGGCGACGT GAACGGCCAC AAGTTCTCTG 4441 TGCGGGGAGA GGGCGAAGGC GACGCCACAA ATGGCAAGCT GACCCTGAAG TTCATCTGCA 4501 CCACCGGCAA GCTGCCCGTG CCTTGGCCTA CCCTCGTGAC CACACTGACC TACGGCGTGC 4561 AGTGCTTCAG CAGATACCCC GACCATATGA AGCGGCACGA CTTCTTCAAG AGCGCCATGC 4621 CCGAGGGCTA CGTGCAGGAA CGGACCATCA GCTTCAAGGA CGACGGCACC TACAAGACCA 4681 GAGCCGAAGT GAAGTTCGAG GGCGACACCC TCGTGAACCG GATCGAGCTG AAGGGCATCG 4741 ATTTCAAAGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA GTACAACTTC AACAGCCACA 4801 ACGTGTACAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGGC 4861 ACAACGTGGA AGATGGCAGC GTGCAGCTGG CCGACCACTA CCAGCAGAAC ACCCCCATCG 4921 GAGATGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGAGC GTGCTGAGCA 4981 AGGACCCCAA CGAGAAGCGG GACCACATGG TGCTGCTGGA ATTCGTGACC GCCGCTGGCA 5041 TCACCCACGG CATGAGCGGC CTGAACGACA TCTTCGAGGC CCAGAAAATC GAGTGGCACG 5101 AGCACCACCA TCATCACCAT CACCACTGAT GATTCGAAAA ATAATAAGGA TCCAATCAAC 5161 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 5221 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 5281 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 5341 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 5401 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 5461 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 5521 CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG 5581 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 5641 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC 5701 TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 5761 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 5821 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 5881 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA 5941 TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT 6001 ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG 6061 AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG 6121 AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC 6181 CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC 6241 GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG 6301 CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC 6361 CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT 6421 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 6481 AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG //