LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2000..2096) /label=attL2 /note="attL2 (c)" misc_signal complement(2111..2130) /note="T7 Promoter/priming site (c)" primer_bind 2138..2154 /note="M13 Reverse priming site" gene 2267..3076 /note="Kanamycin resistance gene" rep_origin 3197..3870 /note="pUC origin" PCR_frame 669..2000 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2020) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2017) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2008..3873,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1978..1998 /note="TEV site" source 673..1977 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:45987 IMAGE:5760636" /tissue_type="Colon, Kidney, Stomach, adult, whole pooled" /clone_lib="NIH_MGC_116" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1977 /gene="HYAL1" /gene_synonym=HYAL-1 /gene_synonym=LUCA1 /gene_synonym=MGC45987 /gene_synonym=NAT6 /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" CDS 673..1977 /dnas_title="hyaluronoglucosaminidase 1" /gene="HYAL1" /gene_synonym=HYAL-1 /gene_synonym=LUCA1 /gene_synonym=MGC45987 /gene_synonym=NAT6 /codon_start=1 /product="hyaluronoglucosaminidase 1" /protein_id="AAH35695.1" /db_xref="GI:23273469" /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" /translation="MAAHLLPICALFLTLLDMAQGFRGPLLPNRPFTTVWNANTQWCL ERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASL IAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHP DWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYT GQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAA GDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQ AIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLT PGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW" misc_feature 673..1977 /note="HYAL1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcagc ccacctgctt cccatctgcg ccctcttcct gaccttactc 721 gatatggccc aaggctttag gggccccttg ctacccaacc ggcccttcac caccgtctgg 781 aatgcaaaca cccagtggtg cctggagagg cacggtgtgg acgtggatgt cagtgtcttc 841 gatgtggtag ccaacccagg gcagaccttc cgcggccctg acatgacaat tttctatagc 901 tcccagctgg gcacctaccc ctactacacg cccactgggg agcctgtgtt tggtggtctg 961 ccccagaatg ccagcctgat tgcccacctg gcccgcacat tccaggacat cctggctgcc 1021 atacctgctc ctgacttctc agggctggca gtcatcgact gggaggcatg gcgcccacgc 1081 tgggccttca actgggacac caaggacatt taccggcagc gctcacgggc actggtacag 1141 gcacagcacc ctgattggcc agctcctcag gtggaggcag tagcccagga ccagttccag 1201 ggagctgcac gggcctggat ggcaggcacc ctccagctgg ggcgggcact gcgtcctcgc 1261 ggcctctggg gcttctatgg cttccctgac tgctacaact atgactttct aagccccaac 1321 tacaccggcc agtgcccatc aggcatccgt gcccaaaatg accagctagg gtggctgtgg 1381 ggccagagcc gtgccctcta tcccagcatc tacatgcccg cagtgctgga gggcacaggg 1441 aagtcacaga tgtatgtgca acaccgtgtg gccgaggcat tccgtgtggc tgtggctgct 1501 ggtgacccca atctgccggt gctgccctat gtccagatct tctatgacac gacaaaccac 1561 tttctgcccc tggatgagct ggagcacagc ctgggggaga gtgcggccca gggggcagct 1621 ggagtggtgc tctgggtgag ctgggaaaat acaagaacca aggaatcatg tcaggccatc 1681 aaggagtata tggacactac actggggccc ttcatcctga acgtgaccag tggggccctt 1741 ctctgcagtc aagccctgtg ctccggccat ggccgctgtg tccgccgcac cagccacccc 1801 aaagccctcc tcctccttaa ccctgccagt ttctccatcc agctcacgcc tggtggtggg 1861 cccctgagcc tgcggggtgc cctctcactt gaagatcagg cacagatggc tgtggagttc 1921 aaatgtcgat gctaccctgg ctggcaggca ccgtggtgtg agcggaagag catgtgggaa 1981 aacttgtact ttcaaggctC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2041 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2101 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2161 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2221 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2281 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2341 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2401 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2461 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 2521 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 2581 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 2641 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 2701 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 2761 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 2821 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 2881 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 2941 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3001 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3061 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3121 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3181 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3241 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3301 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3361 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3421 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3481 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3541 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 3601 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 3661 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 3721 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 3781 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 3841 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //