LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1937..2033) /label=attL2 /note="attL2 (c)" misc_signal complement(2048..2067) /note="T7 Promoter/priming site (c)" primer_bind 2075..2091 /note="M13 Reverse priming site" gene 2204..3013 /note="Kanamycin resistance gene" rep_origin 3134..3807 /note="pUC origin" vector join(1945..3810,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1936 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:45987 IMAGE:5760636" /tissue_type="Colon, Kidney, Stomach, adult, whole pooled" /clone_lib="NIH_MGC_116" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1936 /gene="HYAL1" /gene_synonym=HYAL-1 /gene_synonym=LUCA1 /gene_synonym=MGC45987 /gene_synonym=NAT6 /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" CDS 692..1936 /dnas_title="hyaluronoglucosaminidase 1" /gene="HYAL1" /gene_synonym=HYAL-1 /gene_synonym=LUCA1 /gene_synonym=MGC45987 /gene_synonym=NAT6 /codon_start=1 /product="hyaluronoglucosaminidase 1" /protein_id="AAH35695.1" /db_xref="GI:23273469" /db_xref="GeneID:3373" /db_xref="HGNC:5320" /db_xref="MIM:607071" /translation="MAAHLLPICALFLTLLDMAQGFRGPLLPNRPFTTVWNANTQWCL ERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASL IAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHP DWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYT GQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAA GDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQ AIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLT PGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW" misc_feature 692..1936 /note="HYAL1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctttaggggc cccttgctac ccaaccggcc 721 cttcaccacc gtctggaatg caaacaccca gtggtgcctg gagaggcacg gtgtggacgt 781 ggatgtcagt gtcttcgatg tggtagccaa cccagggcag accttccgcg gccctgacat 841 gacaattttc tatagctccc agctgggcac ctacccctac tacacgccca ctggggagcc 901 tgtgtttggt ggtctgcccc agaatgccag cctgattgcc cacctggccc gcacattcca 961 ggacatcctg gctgccatac ctgctcctga cttctcaggg ctggcagtca tcgactggga 1021 ggcatggcgc ccacgctggg ccttcaactg ggacaccaag gacatttacc ggcagcgctc 1081 acgggcactg gtacaggcac agcaccctga ttggccagct cctcaggtgg aggcagtagc 1141 ccaggaccag ttccagggag ctgcacgggc ctggatggca ggcaccctcc agctggggcg 1201 ggcactgcgt cctcgcggcc tctggggctt ctatggcttc cctgactgct acaactatga 1261 ctttctaagc cccaactaca ccggccagtg cccatcaggc atccgtgccc aaaatgacca 1321 gctagggtgg ctgtggggcc agagccgtgc cctctatccc agcatctaca tgcccgcagt 1381 gctggagggc acagggaagt cacagatgta tgtgcaacac cgtgtggccg aggcattccg 1441 tgtggctgtg gctgctggtg accccaatct gccggtgctg ccctatgtcc agatcttcta 1501 tgacacgaca aaccactttc tgcccctgga tgagctggag cacagcctgg gggagagtgc 1561 ggcccagggg gcagctggag tggtgctctg ggtgagctgg gaaaatacaa gaaccaagga 1621 atcatgtcag gccatcaagg agtatatgga cactacactg gggcccttca tcctgaacgt 1681 gaccagtggg gcccttctct gcagtcaagc cctgtgctcc ggccatggcc gctgtgtccg 1741 ccgcaccagc caccccaaag ccctcctcct ccttaaccct gccagtttct ccatccagct 1801 cacgcctggt ggtgggcccc tgagcctgcg gggtgccctc tcacttgaag atcaggcaca 1861 gatggctgtg gagttcaaat gtcgatgcta ccctggctgg caggcaccgt ggtgtgagcg 1921 gaagagcatg tggtgaCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 1981 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2041 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2101 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2161 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2221 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2281 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2341 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2401 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2461 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2521 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 2581 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 2641 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 2701 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 2761 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 2821 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 2881 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 2941 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3001 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3061 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3121 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3181 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3241 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3301 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3361 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3421 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3481 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3541 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 3601 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3661 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 3721 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 3781 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //