LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2036..2132) /label=attL2 /note="attL2 (c)" misc_signal complement(2147..2166) /note="T7 Promoter/priming site (c)" primer_bind 2174..2190 /note="M13 Reverse priming site" gene 2303..3112 /note="Kanamycin resistance gene" rep_origin 3233..3906 /note="pUC origin" PCR_frame 669..2036 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2056) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2053) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2044..3909,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2014..2034 /note="TEV site" source 673..2013 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1922 IMAGE:3347760" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2013 /gene="HYAL2" /gene_synonym=LuCa-2 /gene_synonym=LUCA2 /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" CDS 673..2013 /dnas_title="hyaluronoglucosaminidase 2" /gene="HYAL2" /gene_synonym=LuCa-2 /gene_synonym=LUCA2 /codon_start=1 /product="hyaluronoglucosaminidase 2" /protein_id="AAH00692.1" /db_xref="GI:12653807" /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" /translation="MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPT QDCGPRLKVPLDLNAFDVQASPNEGFVNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQ NVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPVWVRNWQDKDVYRRLSRQLVA SRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPDCYNHDYVQ NWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEAL RVARTHHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYT TSTETCQYLKDYLTRLLVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTN SFRLVPGHAPGEPQLRPVGELSWADIDHLQTHFRCQCYLGWSGEQCQWDHRQAAGGAS EAWAGSHLTSLLALAALAFTWTL" misc_difference 724..724 /gene="HYAL2" /gene_synonym=LuCa-2 /gene_synonym=LUCA2 /note="'G' in cDNA is 'T' in the human genome; amino acid difference: 'A' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 673..2013 /note="HYAL2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgcgggc aggcccaggc cccaccgtta cattggccct ggtgctggcg 721 gtggcatggg ccatggagct caagcccaca gcaccaccca tcttcactgg ccggcccttt 781 gtggtagcgt gggacgtgcc cacacaggac tgtggcccac gcctcaaggt gccactggac 841 ctgaatgcct ttgatgtgca ggcctcacct aatgagggtt ttgtgaacca gaatattacc 901 atcttctacc gcgaccgtct aggcctgtat ccacgcttcg attctgccgg aaggtctgtg 961 catggtggtg tgccacagaa tgtcagcctt tgggcacacc ggaagatgct gcagaaacgt 1021 gtggagcact acattcggac acaggagtct gcggggctgg cggtcatcga ctgggaggac 1081 tggcgacctg tgtgggtgcg caactggcag gacaaagatg tgtatcgccg gttatcacgc 1141 cagctagtgg ccagtcgtca ccctgactgg cctccagacc gcatagtcaa acaggcacaa 1201 tatgagtttg agttcgcagc acagcagttc atgctggaga cactgcgtta tgtcaaggca 1261 gtgcggcccc ggcacctctg gggcttctac ctctttcctg actgctacaa tcatgattat 1321 gtgcagaact gggagagcta cacaggccgc tgccctgatg ttgaggtggc ccgcaatgac 1381 cagctggcct ggctgtgggc tgagagcacg gccctcttcc cgtctgtcta cctggacgag 1441 acacttgctt cctcccgcca tggccgcaac tttgtgagct tccgtgttca ggaggccctt 1501 cgtgtggctc gcacccacca tgccaaccat gcactcccag tctacgtctt cacacgaccc 1561 acctacagcc gcaggctcac ggggcttagt gagatggacc tcatctctac cattggcgag 1621 agtgcggccc tgggcgcagc tggtgtcatc ctctggggtg acgcggggta caccacaagc 1681 acggagacct gccagtacct caaagattac ctgacacggc tgctggtccc ctacgtggtc 1741 aatgtgtcct gggccaccca atattgcagc cgggcccagt gccatggcca tgggcgctgt 1801 gtgcgccgca accccagtgc cagtaccttc ctgcatctca gcaccaacag tttccgccta 1861 gtgcctggcc atgcacctgg tgaaccccag ctgcgacctg tgggggagct cagttgggcc 1921 gacattgacc acctgcagac acacttccgc tgccagtgct acttgggctg gagtggtgag 1981 caatgccagt gggaccatag gcaggcagct ggagaaaact tgtactttca aggctCCAGC 2041 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2101 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2161 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2221 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2281 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2341 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2401 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2461 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2521 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2581 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2641 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2701 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2761 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2821 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 2881 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 2941 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3001 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3061 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3121 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3181 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3241 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3301 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3361 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3421 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3481 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3541 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3601 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3661 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3721 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3781 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3841 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 3901 TCACATGTT //