LOCUS HYAL2-pGEc2-DEST 6562 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6040..6169) /note="3'-ITR" misc_feature complement(5738..5952) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5190..5731 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4369..4391 /note="attB2" misc_feature 5169..5174 /note="BstB1 site" misc_feature 4392..5087 /note="Super GFP" misc_feature 5088..5138 /note="AviTag" misc_feature 5139..5162 /note="His Tag" PCR_frame 3002..4369 /note="Reading Frame" /set="Set 1" source 3006..4346 /dnas_title="HYAL2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1922 IMAGE:3347760" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4346 /dnas_title="HYAL2" /gene="HYAL2" /gene_synonym="LuCa-2" /gene_synonym="LUCA2" /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" CDS 3006..4346 /dnas_title="hyaluronoglucosaminidase 2" /gene="HYAL2" /gene_synonym="LuCa-2" /gene_synonym="LUCA2" /codon_start=1 /product="hyaluronoglucosaminidase 2" /protein_id="AAH00692.1" /db_xref="GI:12653807" /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" /translation="MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPT QDCGPRLKVPLDLNAFDVQASPNEGFVNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQ NVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPVWVRNWQDKDVYRRLSRQLVA SRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPDCYNHDYVQ NWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEAL RVARTHHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYT TSTETCQYLKDYLTRLLVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTN SFRLVPGHAPGEPQLRPVGELSWADIDHLQTHFRCQCYLGWSGEQCQWDHRQAAGGAS EAWAGSHLTSLLALAALAFTWTL" misc_difference 3057..3057 /dnas_title="HYAL2" /gene="HYAL2" /gene_synonym="LuCa-2" /gene_synonym="LUCA2" /note="'G' in cDNA is 'T' in the human genome; amino acid difference: 'A' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3006..4346 /note="HYAL2 coding region" misc_feature 4347..4367 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4376) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4376) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4377..6562,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4376 /source="HYAL2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgcg ggcaggccca ggccccaccg ttacattggc cctggtgctg gcggtggcat 3061 gggccatgga gctcaagccc acagcaccac ccatcttcac tggccggccc tttgtggtag 3121 cgtgggacgt gcccacacag gactgtggcc cacgcctcaa ggtgccactg gacctgaatg 3181 cctttgatgt gcaggcctca cctaatgagg gttttgtgaa ccagaatatt accatcttct 3241 accgcgaccg tctaggcctg tatccacgct tcgattctgc cggaaggtct gtgcatggtg 3301 gtgtgccaca gaatgtcagc ctttgggcac accggaagat gctgcagaaa cgtgtggagc 3361 actacattcg gacacaggag tctgcggggc tggcggtcat cgactgggag gactggcgac 3421 ctgtgtgggt gcgcaactgg caggacaaag atgtgtatcg ccggttatca cgccagctag 3481 tggccagtcg tcaccctgac tggcctccag accgcatagt caaacaggca caatatgagt 3541 ttgagttcgc agcacagcag ttcatgctgg agacactgcg ttatgtcaag gcagtgcggc 3601 cccggcacct ctggggcttc tacctctttc ctgactgcta caatcatgat tatgtgcaga 3661 actgggagag ctacacaggc cgctgccctg atgttgaggt ggcccgcaat gaccagctgg 3721 cctggctgtg ggctgagagc acggccctct tcccgtctgt ctacctggac gagacacttg 3781 cttcctcccg ccatggccgc aactttgtga gcttccgtgt tcaggaggcc cttcgtgtgg 3841 ctcgcaccca ccatgccaac catgcactcc cagtctacgt cttcacacga cccacctaca 3901 gccgcaggct cacggggctt agtgagatgg acctcatctc taccattggc gagagtgcgg 3961 ccctgggcgc agctggtgtc atcctctggg gtgacgcggg gtacaccaca agcacggaga 4021 cctgccagta cctcaaagat tacctgacac ggctgctggt cccctacgtg gtcaatgtgt 4081 cctgggccac ccaatattgc agccgggccc agtgccatgg ccatgggcgc tgtgtgcgcc 4141 gcaaccccag tgccagtacc ttcctgcatc tcagcaccaa cagtttccgc ctagtgcctg 4201 gccatgcacc tggtgaaccc cagctgcgac ctgtggggga gctcagttgg gccgacattg 4261 accacctgca gacacacttc cgctgccagt gctacttggg ctggagtggt gagcaatgcc 4321 agtgggacca taggcaggca gctggagaaa acttgtactt tcaaggctCC AGCTTTCTTG 4381 TACAAAGTGG TATGAGCAAG GGCGAGGAAC TGTTCACCGG CGTGGTGCCC ATCCTGGTGG 4441 AACTGGATGG CGACGTGAAC GGCCACAAGT TCTCTGTGCG GGGAGAGGGC GAAGGCGACG 4501 CCACAAATGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCTT 4561 GGCCTACCCT CGTGACCACA CTGACCTACG GCGTGCAGTG CTTCAGCAGA TACCCCGACC 4621 ATATGAAGCG GCACGACTTC TTCAAGAGCG CCATGCCCGA GGGCTACGTG CAGGAACGGA 4681 CCATCAGCTT CAAGGACGAC GGCACCTACA AGACCAGAGC CGAAGTGAAG TTCGAGGGCG 4741 ACACCCTCGT GAACCGGATC GAGCTGAAGG GCATCGATTT CAAAGAGGAC GGCAACATCC 4801 TGGGCCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT GTACATCACC GCCGACAAGC 4861 AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGGCACAA CGTGGAAGAT GGCAGCGTGC 4921 AGCTGGCCGA CCACTACCAG CAGAACACCC CCATCGGAGA TGGCCCCGTG CTGCTGCCCG 4981 ACAACCACTA CCTGAGCACC CAGAGCGTGC TGAGCAAGGA CCCCAACGAG AAGCGGGACC 5041 ACATGGTGCT GCTGGAATTC GTGACCGCCG CTGGCATCAC CCACGGCATG AGCGGCCTGA 5101 ACGACATCTT CGAGGCCCAG AAAATCGAGT GGCACGAGCA CCACCATCAT CACCATCACC 5161 ACTGATGATT CGAAAAATAA TAAGGATCCA ATCAACCTCT GGATTACAAA ATTTGTGAAA 5221 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 5281 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 5341 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 5401 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 5461 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 5521 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 5581 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 5641 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 5701 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC TCGACTGTGC CTTCTAGTTG 5761 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 5821 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 5881 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 5941 GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG GATCTTCCTA GAGCATGGCT 6001 ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA GTGATGGAGT 6061 TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC 6121 GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC CTTAATTAAG 6181 AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT TCGCCCTATA GTGAGTCGTA 6241 TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA 6301 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG 6361 CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGAGCGCCCT 6421 GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 6481 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA 6541 AATATTAACG CTTACAATTT AG //