LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2054..2150) /label=attL2 /note="attL2 (c)" misc_signal complement(2165..2184) /note="T7 Promoter/priming site (c)" primer_bind 2192..2208 /note="M13 Reverse priming site" gene 2321..3130 /note="Kanamycin resistance gene" rep_origin 3251..3924 /note="pUC origin" vector join(2062..3927,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2053 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1922 IMAGE:3347760" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2053 /gene="HYAL2" /gene_synonym=LuCa-2 /gene_synonym=LUCA2 /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" CDS 692..2053 /dnas_title="hyaluronoglucosaminidase 2" /gene="HYAL2" /gene_synonym=LuCa-2 /gene_synonym=LUCA2 /codon_start=1 /product="hyaluronoglucosaminidase 2" /protein_id="AAH00692.1" /db_xref="GI:12653807" /db_xref="GeneID:8692" /db_xref="HGNC:5321" /db_xref="MIM:603551" /translation="MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPT QDCGPRLKVPLDLNAFDVQASPNEGFVNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQ NVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPVWVRNWQDKDVYRRLSRQLVA SRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPDCYNHDYVQ NWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEAL RVARTHHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYT TSTETCQYLKDYLTRLLVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTN SFRLVPGHAPGEPQLRPVGELSWADIDHLQTHFRCQCYLGWSGEQCQWDHRQAAGGAS EAWAGSHLTSLLALAALAFTWTL" misc_feature 692..2053 /note="HYAL2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catggagctc aagcccacag caccacccat 721 cttcactggc cggccctttg tggtagcgtg ggacgtgccc acacaggact gtggcccacg 781 cctcaaggtg ccactggacc tgaatgcctt tgatgtgcag gcctcaccta atgagggttt 841 tgtgaaccag aatattacca tcttctaccg cgaccgtcta ggcctgtatc cacgcttcga 901 ttctgccgga aggtctgtgc atggtggtgt gccacagaat gtcagccttt gggcacaccg 961 gaagatgctg cagaaacgtg tggagcacta cattcggaca caggagtctg cggggctggc 1021 ggtcatcgac tgggaggact ggcgacctgt gtgggtgcgc aactggcagg acaaagatgt 1081 gtatcgccgg ttatcacgcc agctagtggc cagtcgtcac cctgactggc ctccagaccg 1141 catagtcaaa caggcacaat atgagtttga gttcgcagca cagcagttca tgctggagac 1201 actgcgttat gtcaaggcag tgcggccccg gcacctctgg ggcttctacc tctttcctga 1261 ctgctacaat catgattatg tgcagaactg ggagagctac acaggccgct gccctgatgt 1321 tgaggtggcc cgcaatgacc agctggcctg gctgtgggct gagagcacgg ccctcttccc 1381 gtctgtctac ctggacgaga cacttgcttc ctcccgccat ggccgcaact ttgtgagctt 1441 ccgtgttcag gaggcccttc gtgtggctcg cacccaccat gccaaccatg cactcccagt 1501 ctacgtcttc acacgaccca cctacagccg caggctcacg gggcttagtg agatggacct 1561 catctctacc attggcgaga gtgcggccct gggcgcagct ggtgtcatcc tctggggtga 1621 cgcggggtac accacaagca cggagacctg ccagtacctc aaagattacc tgacacggct 1681 gctggtcccc tacgtggtca atgtgtcctg ggccacccaa tattgcagcc gggcccagtg 1741 ccatggccat gggcgctgtg tgcgccgcaa ccccagtgcc agtaccttcc tgcatctcag 1801 caccaacagt ttccgcctag tgcctggcca tgcacctggt gaaccccagc tgcgacctgt 1861 gggggagctc agttgggccg acattgacca cctgcagaca cacttccgct gccagtgcta 1921 cttgggctgg agtggtgagc aatgccagtg ggaccatagg caggcagctg gaggtgccag 1981 cgaggcctgg gctgggtccc acctcaccag tctgctggct ctggcagccc tggcctttac 2041 ctggaccttg tagCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 2101 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 2161 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 2221 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2281 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2341 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2401 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2461 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2521 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2581 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2641 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2701 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2761 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2821 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 2881 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 2941 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 3001 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 3061 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 3121 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 3181 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3241 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3301 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3361 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3421 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3481 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3541 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3601 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3661 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3721 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3781 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3841 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 3901 CTTTTGCTGG CCTTTTGCTC ACATGTT //