LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1946..2042) /label=attL2 /note="attL2 (c)" misc_signal complement(2057..2076) /note="T7 Promoter/priming site (c)" primer_bind 2084..2100 /note="M13 Reverse priming site" gene 2213..3022 /note="Kanamycin resistance gene" rep_origin 3143..3816 /note="pUC origin" PCR_frame 669..1946 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1966) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1963) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1954..3819,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1924..1944 /note="TEV site" source 673..1923 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:14472 IMAGE:4104459" /tissue_type="Bone marrow, acute myelogenous leukemia" /clone_lib="NIH_MGC_55" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 673..1923 /gene="HYAL3" /gene_synonym=LUCA-3 /gene_synonym=LUCA14 /gene_synonym=LUCA3 /gene_synonym=Minna14 /db_xref="GeneID:8372" /db_xref="HGNC:5322" /db_xref="MIM:604038" misc_feature 673..1923 /gene="HYAL3" /gene_synonym=LUCA-3 /gene_synonym=LUCA14 /gene_synonym=LUCA3 /gene_synonym=Minna14 /note="This cDNA sequence aligns to the human reference genome (build 35) without differences" CDS 673..1923 /dnas_title="hyaluronoglucosaminidase 3" /gene="HYAL3" /gene_synonym=LUCA-3 /gene_synonym=LUCA14 /gene_synonym=LUCA3 /gene_synonym=Minna14 /codon_start=1 /product="hyaluronoglucosaminidase 3" /protein_id="AAH05896.1" /db_xref="GI:13543477" /db_xref="GeneID:8372" /db_xref="HGNC:5322" /db_xref="MIM:604038" /translation="MTTQLGPALVLGVALCLGCGQPLPQVPERPFSVLWNVPSAHCEA RFGVHLPLNSLGIIANRGQHFHGQNMTIFYKNQLGLYPYFGPRGTAHNGGIPQALPLD RHLALAAYQIHHSLRPGFAGPAVLDWEEWCPLWAGNWGRRRAYQAASWAWAQQVFPDL DPQEQLYKAYTGFEQAARALMEDTLRVAQALRPHGLWGFYHYPACGNGWHSMASNYTG RCHAATLARNTQLHWLWAASSALFPSIYLPPRLPPAHHQAFVRHRLEEAFRVALVGHR HPLPVLAYVRLTHRRSGRFLSQDDLVQSIGVSAALGAAGVVLWGDLSLSSSEEECWHL HDYLVDTLGPYVINVTRAAMACSHQRCHGHGRCARRDPGQMEAFLHLWPDGSLGDWKS FSCHCYWGWAGPTCQEPRPGPKEAV" misc_feature 673..1923 /note="HYAL3 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaccac gcaactgggc ccagccctgg tgctgggggt ggccctgtgc 721 ctgggttgtg gccagcccct accacaggtc cctgaacgcc ccttctctgt gctgtggaat 781 gtaccctcag cacactgtga ggcccgcttt ggtgtgcacc tgccactcaa ttctctgggc 841 atcatagcca accgtggcca gcattttcac ggtcagaaca tgaccatttt ctacaagaac 901 caactcggcc tctatcccta ctttggaccc aggggcacag ctcacaatgg gggcatcccc 961 caggctttgc cccttgaccg ccacctggca ctggctgcct accagatcca ccacagcctg 1021 agacctggct ttgctggccc agcagtgctg gattgggagg agtggtgtcc actctgggct 1081 gggaactggg gccgccgccg agcttatcag gcagcctctt gggcttgggc acagcaggta 1141 ttccctgacc tggaccctca ggagcagctc tacaaggcct atactggctt tgagcaggcg 1201 gcccgtgcac tgatggagga tacgctgcgg gtggcccagg cactacggcc ccatggactc 1261 tggggcttct atcactaccc agcctgtggc aatggctggc atagtatggc ttccaactat 1321 accggccgct gccatgcagc cacccttgcc cgcaacactc aactgcattg gctctgggcc 1381 gcctccagtg ccctcttccc cagcatctac ctcccaccca ggctgccacc tgcccaccac 1441 caggcctttg tccgacatcg cctggaggag gccttccgtg tggcccttgt tgggcaccga 1501 catcccctgc ctgtcctggc ctatgtccgc ctcacacacc ggagatctgg gaggttcctg 1561 tcccaggatg accttgtgca gtccattggt gtgagtgcag cactaggggc agccggcgtg 1621 gtgctctggg gggacctgag cctctccagc tctgaggagg agtgctggca tctccatgac 1681 tacctggtgg acaccttggg cccctatgtg atcaatgtga ccagggcagc gatggcctgc 1741 agtcaccagc ggtgccatgg ccacgggcgc tgtgcccggc gagatccagg acagatggaa 1801 gcctttctac acctgtggcc agacggcagc cttggagatt ggaagtcctt cagctgccac 1861 tgttactggg gctgggctgg ccccacctgc caggagccca ggcctgggcc taaagaagca 1921 gtagaaaact tgtactttca aggctCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 1981 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2041 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2101 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2161 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2221 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2281 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2341 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2401 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2461 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2521 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2581 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2641 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2701 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2761 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2821 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 2881 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 2941 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3001 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3061 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3121 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3181 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3241 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3301 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3361 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3421 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3481 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3541 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3601 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3661 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3721 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3781 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //