LOCUS HYAL3-pGEc2-DEST 6472 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5950..6079) /note="3'-ITR" misc_feature complement(5648..5862) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5100..5641 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4279..4301 /note="attB2" misc_feature 5079..5084 /note="BstB1 site" misc_feature 4302..4997 /note="Super GFP" misc_feature 4998..5048 /note="AviTag" misc_feature 5049..5072 /note="His Tag" PCR_frame 3002..4279 /note="Reading Frame" /set="Set 1" source 3006..4256 /dnas_title="HYAL3-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:14472 IMAGE:4104459" /tissue_type="Bone marrow, acute myelogenous leukemia" /clone_lib="NIH_MGC_55" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 3006..4256 /dnas_title="HYAL3" /gene="HYAL3" /gene_synonym="LUCA-3" /gene_synonym="LUCA14" /gene_synonym="LUCA3" /gene_synonym="Minna14" /db_xref="GeneID:8372" /db_xref="HGNC:5322" /db_xref="MIM:604038" misc_feature 3006..4256 /dnas_title="HYAL3" /gene="HYAL3" /gene_synonym="LUCA-3" /gene_synonym="LUCA14" /gene_synonym="LUCA3" /gene_synonym="Minna14" /note="This cDNA sequence aligns to the human reference genome (build 35) without differences" CDS 3006..4256 /dnas_title="hyaluronoglucosaminidase 3" /gene="HYAL3" /gene_synonym="LUCA-3" /gene_synonym="LUCA14" /gene_synonym="LUCA3" /gene_synonym="Minna14" /codon_start=1 /product="hyaluronoglucosaminidase 3" /protein_id="AAH05896.1" /db_xref="GI:13543477" /db_xref="GeneID:8372" /db_xref="HGNC:5322" /db_xref="MIM:604038" /translation="MTTQLGPALVLGVALCLGCGQPLPQVPERPFSVLWNVPSAHCEA RFGVHLPLNSLGIIANRGQHFHGQNMTIFYKNQLGLYPYFGPRGTAHNGGIPQALPLD RHLALAAYQIHHSLRPGFAGPAVLDWEEWCPLWAGNWGRRRAYQAASWAWAQQVFPDL DPQEQLYKAYTGFEQAARALMEDTLRVAQALRPHGLWGFYHYPACGNGWHSMASNYTG RCHAATLARNTQLHWLWAASSALFPSIYLPPRLPPAHHQAFVRHRLEEAFRVALVGHR HPLPVLAYVRLTHRRSGRFLSQDDLVQSIGVSAALGAAGVVLWGDLSLSSSEEECWHL HDYLVDTLGPYVINVTRAAMACSHQRCHGHGRCARRDPGQMEAFLHLWPDGSLGDWKS FSCHCYWGWAGPTCQEPRPGPKEAV" misc_feature 3006..4256 /note="HYAL3 coding region" misc_feature 4257..4277 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4286) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4286) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4287..6472,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4286 /source="HYAL3-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgac cacgcaactg ggcccagccc tggtgctggg ggtggccctg tgcctgggtt 3061 gtggccagcc cctaccacag gtccctgaac gccccttctc tgtgctgtgg aatgtaccct 3121 cagcacactg tgaggcccgc tttggtgtgc acctgccact caattctctg ggcatcatag 3181 ccaaccgtgg ccagcatttt cacggtcaga acatgaccat tttctacaag aaccaactcg 3241 gcctctatcc ctactttgga cccaggggca cagctcacaa tgggggcatc ccccaggctt 3301 tgccccttga ccgccacctg gcactggctg cctaccagat ccaccacagc ctgagacctg 3361 gctttgctgg cccagcagtg ctggattggg aggagtggtg tccactctgg gctgggaact 3421 ggggccgccg ccgagcttat caggcagcct cttgggcttg ggcacagcag gtattccctg 3481 acctggaccc tcaggagcag ctctacaagg cctatactgg ctttgagcag gcggcccgtg 3541 cactgatgga ggatacgctg cgggtggccc aggcactacg gccccatgga ctctggggct 3601 tctatcacta cccagcctgt ggcaatggct ggcatagtat ggcttccaac tataccggcc 3661 gctgccatgc agccaccctt gcccgcaaca ctcaactgca ttggctctgg gccgcctcca 3721 gtgccctctt ccccagcatc tacctcccac ccaggctgcc acctgcccac caccaggcct 3781 ttgtccgaca tcgcctggag gaggccttcc gtgtggccct tgttgggcac cgacatcccc 3841 tgcctgtcct ggcctatgtc cgcctcacac accggagatc tgggaggttc ctgtcccagg 3901 atgaccttgt gcagtccatt ggtgtgagtg cagcactagg ggcagccggc gtggtgctct 3961 ggggggacct gagcctctcc agctctgagg aggagtgctg gcatctccat gactacctgg 4021 tggacacctt gggcccctat gtgatcaatg tgaccagggc agcgatggcc tgcagtcacc 4081 agcggtgcca tggccacggg cgctgtgccc ggcgagatcc aggacagatg gaagcctttc 4141 tacacctgtg gccagacggc agccttggag attggaagtc cttcagctgc cactgttact 4201 ggggctgggc tggccccacc tgccaggagc ccaggcctgg gcctaaagaa gcagtagaaa 4261 acttgtactt tcaaggctCC AGCTTTCTTG TACAAAGTGG TATGAGCAAG GGCGAGGAAC 4321 TGTTCACCGG CGTGGTGCCC ATCCTGGTGG AACTGGATGG CGACGTGAAC GGCCACAAGT 4381 TCTCTGTGCG GGGAGAGGGC GAAGGCGACG CCACAAATGG CAAGCTGACC CTGAAGTTCA 4441 TCTGCACCAC CGGCAAGCTG CCCGTGCCTT GGCCTACCCT CGTGACCACA CTGACCTACG 4501 GCGTGCAGTG CTTCAGCAGA TACCCCGACC ATATGAAGCG GCACGACTTC TTCAAGAGCG 4561 CCATGCCCGA GGGCTACGTG CAGGAACGGA CCATCAGCTT CAAGGACGAC GGCACCTACA 4621 AGACCAGAGC CGAAGTGAAG TTCGAGGGCG ACACCCTCGT GAACCGGATC GAGCTGAAGG 4681 GCATCGATTT CAAAGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AACTTCAACA 4741 GCCACAACGT GTACATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA 4801 TCCGGCACAA CGTGGAAGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG CAGAACACCC 4861 CCATCGGAGA TGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGAGCGTGC 4921 TGAGCAAGGA CCCCAACGAG AAGCGGGACC ACATGGTGCT GCTGGAATTC GTGACCGCCG 4981 CTGGCATCAC CCACGGCATG AGCGGCCTGA ACGACATCTT CGAGGCCCAG AAAATCGAGT 5041 GGCACGAGCA CCACCATCAT CACCATCACC ACTGATGATT CGAAAAATAA TAAGGATCCA 5101 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 5161 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 5221 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 5281 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 5341 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 5401 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 5461 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 5521 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 5581 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 5641 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 5701 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 5761 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 5821 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 5881 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 5941 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 6001 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 6061 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 6121 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 6181 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 6241 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 6301 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 6361 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 6421 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //