LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1967..2063) /label=attL2 /note="attL2 (c)" misc_signal complement(2078..2097) /note="T7 Promoter/priming site (c)" primer_bind 2105..2121 /note="M13 Reverse priming site" gene 2234..3043 /note="Kanamycin resistance gene" rep_origin 3164..3837 /note="pUC origin" vector join(1975..3840,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1963 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:132448 IMAGE:8143791" /tissue_type="Cerebellum, Uterus, Testis, PCR rescued clones" /clone_lib="NIH_MGC_337" /lab_host="DH10B" /note="Vector: pCR4-TOPO; Clone identification sequence tag: CCGCCAAC" gene 692..1963 /gene="HYAL4" /db_xref="GeneID:23553" /db_xref="HGNC:5323" /db_xref="MIM:604510" CDS 692..1963 /dnas_title="hyaluronoglucosaminidase 4" /gene="HYAL4" /codon_start=1 /product="hyaluronoglucosaminidase 4" /protein_id="AAI04789.1" /db_xref="GI:85396869" /db_xref="GeneID:23553" /db_xref="HGNC:5323" /db_xref="MIM:604510" /translation="MKVLSEGQLKLCVVQPVHLTSWLLIFFILKSISCLKPARLPIYQ RKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTIFYVNRLGYYPWYT SQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYWRPQWARNWNSK DVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSRPKGLWGYY LYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILR FSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAA GIVIWGDMNLTSSKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMW NAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREIK TADGCSGVSPSPGSLMTLCLLLLASYRSIQL" misc_feature 692..1963 /note="HYAL4 coding region" misc_difference 1640..1640 /gene="HYAL4" /note="'T' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'A' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caagtctatc tcttgtctaa aacctgctcg 721 acttccaatt tatcaaagga aaccttttat agctgcttgg aatgctccaa cagatcagtg 781 tttgataaaa tataatttaa gactaaattt gaaaatgttt cctgtgattg gaagcccact 841 ggccaaggcc agggggcaaa atgtcactat attttatgtc aacagattgg gatactatcc 901 gtggtataca tcacaagggg tccccattaa tggaggtctc ccacagaaca taagtttaca 961 agtacatctg gaaaaagctg accaagatat taattattac atccctgctg aagatttcag 1021 tggacttgct gttatagatt gggaatattg gcgaccacag tgggcccgga actggaactc 1081 aaaagatgtt tacagacaga agtcaagaaa gcttatttcc gatatgggaa agaatgtatc 1141 agctaccgat attgaatatt tagccaaagt gacctttgaa gaaagtgcaa aagctttcat 1201 gaaggaaacc atcaaattgg gaattaagag ccgacccaaa ggcctttggg gttattattt 1261 atatcctgat tgccacaatt ataacgttta tgccccaaac tactctgggt catgcccaga 1321 agacgaagtc ttgaggaaca atgagctctc ttggctctgg aacagcagtg ctgctttata 1381 tccttctatc ggtgtctgga aatcccttgg agacagtgaa aacattttgc gcttctccaa 1441 atttcgggtg catgaatcca tgaggatctc caccatgaca tctcatgatt atgctctgcc 1501 tgtatttgtc tacacaaggc tagggtacag agatgaacct ttatttttcc tttctaagca 1561 agatctagtc agcaccatag gagaaagtgc tgccttggga gctgcaggca ttgttatttg 1621 gggagacatg aatttaactt catccaaggc caactgtaca aaggtgaagc agtttgtgag 1681 ttctgattta gggagctaca tagccaatgt gaccagagct gctgaggtat gcagccttca 1741 cctctgcagg aacaatggca ggtgcataag gaagatgtgg aacgcgccca gttaccttca 1801 cttgaaccct gcaagttacc acatagaggc ctctgaggac ggggagttta ctgtgaaagg 1861 aaaagcatct gatacagacc tggcagtgat ggcagataca ttttcctgtc attgttatca 1921 gggatatgaa ggagctgatt gcagagaaat aaagacggct gattgaCCAG CTTTCTTGTA 1981 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2041 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2101 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2161 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2221 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2281 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2341 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2401 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2461 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 2521 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 2581 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 2641 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 2701 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 2761 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 2821 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 2881 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 2941 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3001 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3061 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3121 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3181 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3241 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3301 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3361 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3421 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3481 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3541 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3601 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3661 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 3721 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 3781 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //