LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2162..2258) /label=attL2 /note="attL2 (c)" misc_signal complement(2273..2292) /note="T7 Promoter/priming site (c)" primer_bind 2300..2316 /note="M13 Reverse priming site" gene 2429..3238 /note="Kanamycin resistance gene" rep_origin 3359..4032 /note="pUC origin" PCR_frame 669..2162 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2182) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2179) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2170..4035,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2140..2160 /note="TEV site" source 673..2139 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26532 IMAGE:4838230" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 673..2139 /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" CDS 673..2139 /dnas_title="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /codon_start=1 /product="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /protein_id="AAH26163.1" /db_xref="GI:20072878" /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" /translation="MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVI PNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYI DSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKP KDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGY YLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATL YVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGAS GIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNW NSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRI GFF" misc_feature 673..2139 /note="SPAM1 coding region" misc_difference 1254..1254 /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgggagt gctaaaattc aagcacatct ttttcagaag ctttgttaaa 721 tcaagtggag tatcccagat agttttcacc ttccttctga ttccatgttg cttgactctg 781 aatttcagag cacctcctgt tattccaaat gtgcctttcc tctgggcctg gaatgcccca 841 agtgaatttt gtcttggaaa atttgatgag ccactagata tgagcctctt ctctttcata 901 ggaagccccc gaataaacgc caccgggcaa ggtgttacaa tattttatgt tgatagactt 961 ggctactatc cttacataga ttcaatcaca ggagtaactg tgaatggagg aatcccccag 1021 aagatttcct tacaagacca tctggacaaa gctaagaaag acattacatt ttatatgcca 1081 gtagacaatt tgggaatggc tgttattgac tgggaagaat ggagacccac ttgggcaaga 1141 aactggaaac ctaaagatgt ttacaagaat aggtctattg aattggttca gcaacaaaat 1201 gtacaactta gtctcacaga ggccactgag aaagcaaaac aagaatttga aaaagcaggg 1261 aaggatttcc tggtagagac tataaaattg ggaaaattac ttcggccaaa tcacttgtgg 1321 ggttattatc tttttccgga ttgttacaac catcactata agaaacccgg ttacaatgga 1381 agttgcttca atgtagaaat aaaaagaaat gatgatctca gctggttgtg gaatgaaagc 1441 actgctcttt acccatccat ttatttgaac actcagcagt ctcctgtagc tgctacactc 1501 tatgtgcgca atcgagttcg ggaagccatc agagtttcca aaatacctga tgcaaaaagt 1561 ccacttccgg tttttgcata tacccgcata gtttttactg atcaagtttt gaaattcctt 1621 tctcaagatg aacttgtgta tacatttggc gaaactgttg ctctgggtgc ttctggaatt 1681 gtaatatggg gaaccctcag tataatgcga agtatgaaat cttgcttgct cctagacaat 1741 tacatggaga ctatactgaa tccttacata atcaacgtca cactagcagc caaaatgtgt 1801 agccaagtgc tttgccagga gcaaggagtg tgtataagga aaaactggaa ttcaagtgac 1861 tatcttcacc tcaacccaga taattttgct attcaacttg agaaaggtgg aaagttcaca 1921 gtacgtggaa aaccgacact tgaagacctg gagcaatttt ctgaaaaatt ttattgcagc 1981 tgttatagca ccttgagttg taaggagaaa gctgatgtaa aagacactga tgctgttgat 2041 gtgtgtattg ctgatggtgt ctgtatagat gcttttctaa aacctcccat ggagacagaa 2101 gaacctcaaa ttttctacaa tgcttcaccc tccacactag aaaacttgta ctttcaaggc 2161 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2221 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2281 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2341 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2401 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2461 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2521 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2581 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2641 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2701 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2761 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2821 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2881 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2941 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3001 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3061 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3121 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3181 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3241 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3301 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3361 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3421 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3481 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3541 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3601 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3661 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3721 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3781 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3841 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3901 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3961 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4021 TTTTGCTCAC ATGTT //