LOCUS SPAM1-pGEc1-DEST 5962 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5440..5569) /note="3'-ITR" misc_feature complement(5138..5352) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4590..5131 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4495..4517 /label=attB2 /note="attB2 site" misc_feature 4527..4550 /note="8xHis Tag" misc_feature 4554..4577 /note="StrepTag" PCR_frame 3002..4495 /note="Reading Frame" /set="Set 1" source 3006..4472 /dnas_title="SPAM1-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26532 IMAGE:4838230" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3006..4472 /dnas_title="SPAM1" /gene="SPAM1" /gene_synonym="HYA1" /gene_synonym="HYAL1" /gene_synonym="HYAL3" /gene_synonym="MGC26532" /gene_synonym="PH-20" /gene_synonym="PH20" /gene_synonym="SPAG15" /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" CDS 3006..4472 /dnas_title="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /gene="SPAM1" /gene_synonym="HYA1" /gene_synonym="HYAL1" /gene_synonym="HYAL3" /gene_synonym="MGC26532" /gene_synonym="PH-20" /gene_synonym="PH20" /gene_synonym="SPAG15" /codon_start=1 /product="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /protein_id="AAH26163.1" /db_xref="GI:20072878" /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" /translation="MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVI PNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYI DSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKP KDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGY YLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATL YVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGAS GIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNW NSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRI GFF" misc_feature 3006..4472 /note="SPAM1 coding region" misc_difference 3587..3587 /dnas_title="SPAM1" /gene="SPAM1" /gene_synonym="HYA1" /gene_synonym="HYAL1" /gene_synonym="HYAL3" /gene_synonym="MGC26532" /gene_synonym="PH-20" /gene_synonym="PH20" /gene_synonym="SPAG15" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4473..4493 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4502) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4502) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4503..5962,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4502 /source="SPAM1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggg agtgctaaaa ttcaagcaca tctttttcag aagctttgtt aaatcaagtg 3061 gagtatccca gatagttttc accttccttc tgattccatg ttgcttgact ctgaatttca 3121 gagcacctcc tgttattcca aatgtgcctt tcctctgggc ctggaatgcc ccaagtgaat 3181 tttgtcttgg aaaatttgat gagccactag atatgagcct cttctctttc ataggaagcc 3241 cccgaataaa cgccaccggg caaggtgtta caatatttta tgttgataga cttggctact 3301 atccttacat agattcaatc acaggagtaa ctgtgaatgg aggaatcccc cagaagattt 3361 ccttacaaga ccatctggac aaagctaaga aagacattac attttatatg ccagtagaca 3421 atttgggaat ggctgttatt gactgggaag aatggagacc cacttgggca agaaactgga 3481 aacctaaaga tgtttacaag aataggtcta ttgaattggt tcagcaacaa aatgtacaac 3541 ttagtctcac agaggccact gagaaagcaa aacaagaatt tgaaaaagca gggaaggatt 3601 tcctggtaga gactataaaa ttgggaaaat tacttcggcc aaatcacttg tggggttatt 3661 atctttttcc ggattgttac aaccatcact ataagaaacc cggttacaat ggaagttgct 3721 tcaatgtaga aataaaaaga aatgatgatc tcagctggtt gtggaatgaa agcactgctc 3781 tttacccatc catttatttg aacactcagc agtctcctgt agctgctaca ctctatgtgc 3841 gcaatcgagt tcgggaagcc atcagagttt ccaaaatacc tgatgcaaaa agtccacttc 3901 cggtttttgc atatacccgc atagttttta ctgatcaagt tttgaaattc ctttctcaag 3961 atgaacttgt gtatacattt ggcgaaactg ttgctctggg tgcttctgga attgtaatat 4021 ggggaaccct cagtataatg cgaagtatga aatcttgctt gctcctagac aattacatgg 4081 agactatact gaatccttac ataatcaacg tcacactagc agccaaaatg tgtagccaag 4141 tgctttgcca ggagcaagga gtgtgtataa ggaaaaactg gaattcaagt gactatcttc 4201 acctcaaccc agataatttt gctattcaac ttgagaaagg tggaaagttc acagtacgtg 4261 gaaaaccgac acttgaagac ctggagcaat tttctgaaaa attttattgc agctgttata 4321 gcaccttgag ttgtaaggag aaagctgatg taaaagacac tgatgctgtt gatgtgtgta 4381 ttgctgatgg tgtctgtata gatgcttttc taaaacctcc catggagaca gaagaacctc 4441 aaattttcta caatgcttca ccctccacac tagaaaactt gtactttcaa ggctCCAGCT 4501 TTCTTGTACA AAGTGGTGAT GGCCGCCATC ATCATCATCA TCATCATCAT GCGTGGAGCC 4561 ACCCGCAGTT CGAAAAATAA TAAGGATCCA ATCAACCTCT GGATTACAAA ATTTGTGAAA 4621 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 4681 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 4741 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 4801 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 4861 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 4921 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 4981 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 5041 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 5101 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC TCGACTGTGC CTTCTAGTTG 5161 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 5221 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 5281 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 5341 GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG GATCTTCCTA GAGCATGGCT 5401 ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA GTGATGGAGT 5461 TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC 5521 GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC CTTAATTAAG 5581 AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT TCGCCCTATA GTGAGTCGTA 5641 TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA 5701 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG 5761 CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGAGCGCCCT 5821 GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 5881 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA 5941 AATATTAACG CTTACAATTT AG //