LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2057..2153) /label=attL2 /note="attL2 (c)" misc_signal complement(2168..2187) /note="T7 Promoter/priming site (c)" primer_bind 2195..2211 /note="M13 Reverse priming site" gene 2324..3133 /note="Kanamycin resistance gene" rep_origin 3254..3927 /note="pUC origin" vector join(2065..3930,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2056 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26532 IMAGE:4838230" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..2056 /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" CDS 692..2056 /dnas_title="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /codon_start=1 /product="sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)" /protein_id="AAH26163.1" /db_xref="GI:20072878" /db_xref="GeneID:6677" /db_xref="HGNC:11217" /db_xref="MIM:600930" /translation="MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVI PNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYI DSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKP KDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGY YLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATL YVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGAS GIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNW NSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIWRLEVWDQGISRI GFF" misc_difference 1168..1168 /gene="SPAM1" /gene_synonym=HYA1 /gene_synonym=HYAL1 /gene_synonym=HYAL3 /gene_synonym=MGC26532 /gene_synonym=PH-20 /gene_synonym=PH20 /gene_synonym=SPAG15 /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..2056 /note="SPAM1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cctgaatttc agagcacctc ctgttattcc 721 aaatgtgcct ttcctctggg cctggaatgc cccaagtgaa ttttgtcttg gaaaatttga 781 tgagccacta gatatgagcc tcttctcttt cataggaagc ccccgaataa acgccaccgg 841 gcaaggtgtt acaatatttt atgttgatag acttggctac tatccttaca tagattcaat 901 cacaggagta actgtgaatg gaggaatccc ccagaagatt tccttacaag accatctgga 961 caaagctaag aaagacatta cattttatat gccagtagac aatttgggaa tggctgttat 1021 tgactgggaa gaatggagac ccacttgggc aagaaactgg aaacctaaag atgtttacaa 1081 gaataggtct attgaattgg ttcagcaaca aaatgtacaa cttagtctca cagaggccac 1141 tgagaaagca aaacaagaat ttgaaaaagc agggaaggat ttcctggtag agactataaa 1201 attgggaaaa ttacttcggc caaatcactt gtggggttat tatctttttc cggattgtta 1261 caaccatcac tataagaaac ccggttacaa tggaagttgc ttcaatgtag aaataaaaag 1321 aaatgatgat ctcagctggt tgtggaatga aagcactgct ctttacccat ccatttattt 1381 gaacactcag cagtctcctg tagctgctac actctatgtg cgcaatcgag ttcgggaagc 1441 catcagagtt tccaaaatac ctgatgcaaa aagtccactt ccggtttttg catatacccg 1501 catagttttt actgatcaag ttttgaaatt cctttctcaa gatgaacttg tgtatacatt 1561 tggcgaaact gttgctctgg gtgcttctgg aattgtaata tggggaaccc tcagtataat 1621 gcgaagtatg aaatcttgct tgctcctaga caattacatg gagactatac tgaatcctta 1681 cataatcaac gtcacactag cagccaaaat gtgtagccaa gtgctttgcc aggagcaagg 1741 agtgtgtata aggaaaaact ggaattcaag tgactatctt cacctcaacc cagataattt 1801 tgctattcaa cttgagaaag gtggaaagtt cacagtacgt ggaaaaccga cacttgaaga 1861 cctggagcaa ttttctgaaa aattttattg cagctgttat agcaccttga gttgtaagga 1921 gaaagctgat gtaaaagaca ctgatgctgt tgatgtgtgt attgctgatg gtgtctgtat 1981 agatgctttt ctaaaacctc ccatggagac agaagaacct caaattttct acaatgcttc 2041 accctccaca ctatgaCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 2101 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2161 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2221 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2281 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2341 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2401 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2461 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2521 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2581 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2641 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 2701 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 2761 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 2821 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 2881 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 2941 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 3001 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 3061 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3121 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3181 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3241 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3301 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3361 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3421 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3481 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3541 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3601 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3661 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 3721 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3781 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 3841 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 3901 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //