LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2624..2720) /label=attL2 /note="attL2 (c)" misc_signal complement(2735..2754) /note="T7 Promoter/priming site (c)" primer_bind 2762..2778 /note="M13 Reverse priming site" gene 2891..3700 /note="Kanamycin resistance gene" rep_origin 3821..4494 /note="pUC origin" vector join(2632..4497,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2623 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:33117 IMAGE:5267705" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..2623 /gene="GALC" /db_xref="GeneID:2581" /db_xref="HGNC:4115" /db_xref="MIM:606890" CDS 692..2623 /dnas_title="GALC protein" /gene="GALC" /codon_start=1 /product="GALC protein" /protein_id="AAH36518.1" /db_xref="GI:22328079" /db_xref="GeneID:2581" /db_xref="HGNC:4115" /db_xref="MIM:606890" /translation="MTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGA VSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMH YALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVV TWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESIS ASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRIL NQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQF TQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQF ATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTL TTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPG EHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTTKCDVYIETPDTGGVFIAGRV NKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHF ASGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR" misc_difference 895..895 /gene="GALC" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..2623 /note="GALC coding region" misc_difference 2185..2185 /gene="GALC" /note="'G' in cDNA is 'A' in the human genome; no amino acid change." misc_difference 2250..2250 /gene="GALC" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'T' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2263..2263 /gene="GALC" /note="'T' in cDNA is 'A' in the human genome; no amino acid change." misc_difference 2486..2486 /gene="GALC" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'A' in cDNA, 'T' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctacgtgctc gacgactccg acgggctggg 721 ccgggagttc gacggcatcg gcgcggtcag cggcggcggg gcaacctccc gacttctagt 781 aaattaccca gagccctatc gttctcagat attggattat ctctttaagc cgaattttgg 841 tgcctctttg catattttaa aagtggaaat aggtggtgat gggcagacaa cagatggcac 901 tgagccctcc cacatgcatt atgcactaga tgagaattat ttccgaggat acgagtggtg 961 gttgatgaaa gaagctaaga agaggaatcc caatattaca ctcattgggt tgccatggtc 1021 attccctgga tggctgggaa aaggtttcga ctggccttat gtcaatcttc agctgactgc 1081 ctattatgtc gtgacctgga ttgtgggcgc caagcgttac catgatttgg acattgatta 1141 tattggaatt tggaatgaga ggtcatataa tgccaattat attaagatat taagaaaaat 1201 gctgaattat caaggtctcc agcgagtgaa aatcatagca agtgataatc tctgggagtc 1261 catctctgca tccatgctcc ttgatgccga actcttcaag gtggttgatg ttataggggc 1321 tcattatcct ggaacccatt cagcaaaaga tgcaaagttg actgggaaga agctttggtc 1381 ttctgaagac tttagcactt taaatagtga catgggtgca ggctgctggg gtcgcatttt 1441 aaatcagaat tatatcaatg gctatatgac ttccacaatc gcatggaatt tagtggctag 1501 ttactatgaa cagttgcctt atgggagatg cgggttgatg acggcccagg agccatggag 1561 tgggcactac gtggtagaat ctcctgtctg ggtatcagct cataccactc agtttactca 1621 acctggctgg tattacctga agacagttgg ccatttagag aaaggaggaa gctacgtagc 1681 tctgactgat ggcttaggga acctcaccat catcattgaa accatgagtc ataaacattc 1741 taagtgcata cggccatttc ttccttattt caatgtgtca caacaatttg ccacctttgt 1801 tcttaaggga tcttttagtg aaataccaga gctacaggta tggtatacca aacttggaaa 1861 aacatccgaa agatttcttt ttaagcagct ggattctcta tggctccttg acagcgatgg 1921 cagtttcaca ctgagcctgc atgaagatga gctgttcaca ctcaccactc tcaccactgg 1981 tcgcaaaggc agctacccgc ttcctccaaa atcccagccc ttcccaagta cctataagga 2041 tgatttcaat gttgattacc cattttttag tgaagctcca aactttgctg atcaaactgg 2101 tgtatttgaa tattttacaa atattgaaga ccctggcgag catcacttca cgctacgcca 2161 agttctcaac cagagaccca ttacgtgggc tgccgatgca tccaacacaa tcagtattat 2221 aggagactac aactggacca atctgactac aaagtgtgat gtttacatag agacccctga 2281 cacaggaggt gtgttcattg caggaagagt aaataaaggt ggtattttga ttagaagtgc 2341 cagaggaatt ttcttctgga tttttgcaaa tggatcttac agggttacag gtgatttagc 2401 tggatggatt atatatgctt taggacgtgt tgaagttaca gcaaaaaaat ggtatacact 2461 cacgttaact attaagggtc atttcgcctc tggcatgctg aatgacaagt ctctgtggac 2521 agacatccct gtgaattttc caaagaatgg ctgggctgca attggaactc actcctttga 2581 atttgcacag tttgacaact ttcttgtgga agccacacgc taaCCAGCTT TCTTGTACAA 2641 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 2701 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 2761 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 2821 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 2881 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 2941 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 3001 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 3061 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 3121 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 3181 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 3241 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 3301 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 3361 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 3421 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 3481 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 3541 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 3601 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 3661 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 3721 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 3781 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3841 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3901 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3961 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 4021 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4081 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4141 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4201 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 4261 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4321 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4381 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 4441 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //