LOCUS GALC-pGEn2-DEST 7233 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5861..6402 /note="WPRE" misc_recomb 5823..5845 /label=attB2 /note="attB2 site" misc_feature complement(6409..6623) /note="BGH pA" misc_feature complement(6711..6840) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5822 /dnas_title="GALC-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:33117 IMAGE:5267705" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3891..5822 /dnas_title="GALC" /gene="GALC" /db_xref="GeneID:2581" /db_xref="HGNC:4115" /db_xref="MIM:606890" CDS 3891..5822 /dnas_title="GALC protein" /gene="GALC" /codon_start=1 /product="GALC protein" /protein_id="AAH36518.1" /db_xref="GI:22328079" /db_xref="GeneID:2581" /db_xref="HGNC:4115" /db_xref="MIM:606890" /translation="MTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGA VSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMH YALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVV TWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESIS ASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRIL NQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQF TQPGWYYLKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQF ATFVLKGSFSEIPELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTL TTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPG EHHFTLRQVLNQRPITWAADASNTISIIGDYNWTNLTTKCDVYIETPDTGGVFIAGRV NKGGILIRSARGIFFWIFANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHF ASGMLNDKSLWTDIPVNFPKNGWAAIGTHSFEFAQFDNFLVEATR" misc_difference 4094..4094 /dnas_title="GALC" /gene="GALC" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3891..5822 /note="GALC coding region" misc_difference 5384..5384 /dnas_title="GALC" /gene="GALC" /note="'G' in cDNA is 'A' in the human genome; no amino acid change." misc_difference 5449..5449 /dnas_title="GALC" /gene="GALC" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'T' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 5462..5462 /dnas_title="GALC" /gene="GALC" /note="'T' in cDNA is 'A' in the human genome; no amino acid change." misc_difference 5685..5685 /dnas_title="GALC" /gene="GALC" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'A' in cDNA, 'T' in the human genome." vector join(5831..7233,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5830 /source="GALC-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc tacgtgctcg 3901 acgactccga cgggctgggc cgggagttcg acggcatcgg cgcggtcagc ggcggcgggg 3961 caacctcccg acttctagta aattacccag agccctatcg ttctcagata ttggattatc 4021 tctttaagcc gaattttggt gcctctttgc atattttaaa agtggaaata ggtggtgatg 4081 ggcagacaac agatggcact gagccctccc acatgcatta tgcactagat gagaattatt 4141 tccgaggata cgagtggtgg ttgatgaaag aagctaagaa gaggaatccc aatattacac 4201 tcattgggtt gccatggtca ttccctggat ggctgggaaa aggtttcgac tggccttatg 4261 tcaatcttca gctgactgcc tattatgtcg tgacctggat tgtgggcgcc aagcgttacc 4321 atgatttgga cattgattat attggaattt ggaatgagag gtcatataat gccaattata 4381 ttaagatatt aagaaaaatg ctgaattatc aaggtctcca gcgagtgaaa atcatagcaa 4441 gtgataatct ctgggagtcc atctctgcat ccatgctcct tgatgccgaa ctcttcaagg 4501 tggttgatgt tataggggct cattatcctg gaacccattc agcaaaagat gcaaagttga 4561 ctgggaagaa gctttggtct tctgaagact ttagcacttt aaatagtgac atgggtgcag 4621 gctgctgggg tcgcatttta aatcagaatt atatcaatgg ctatatgact tccacaatcg 4681 catggaattt agtggctagt tactatgaac agttgcctta tgggagatgc gggttgatga 4741 cggcccagga gccatggagt gggcactacg tggtagaatc tcctgtctgg gtatcagctc 4801 ataccactca gtttactcaa cctggctggt attacctgaa gacagttggc catttagaga 4861 aaggaggaag ctacgtagct ctgactgatg gcttagggaa cctcaccatc atcattgaaa 4921 ccatgagtca taaacattct aagtgcatac ggccatttct tccttatttc aatgtgtcac 4981 aacaatttgc cacctttgtt cttaagggat cttttagtga aataccagag ctacaggtat 5041 ggtataccaa acttggaaaa acatccgaaa gatttctttt taagcagctg gattctctat 5101 ggctccttga cagcgatggc agtttcacac tgagcctgca tgaagatgag ctgttcacac 5161 tcaccactct caccactggt cgcaaaggca gctacccgct tcctccaaaa tcccagccct 5221 tcccaagtac ctataaggat gatttcaatg ttgattaccc attttttagt gaagctccaa 5281 actttgctga tcaaactggt gtatttgaat attttacaaa tattgaagac cctggcgagc 5341 atcacttcac gctacgccaa gttctcaacc agagacccat tacgtgggct gccgatgcat 5401 ccaacacaat cagtattata ggagactaca actggaccaa tctgactaca aagtgtgatg 5461 tttacataga gacccctgac acaggaggtg tgttcattgc aggaagagta aataaaggtg 5521 gtattttgat tagaagtgcc agaggaattt tcttctggat ttttgcaaat ggatcttaca 5581 gggttacagg tgatttagct ggatggatta tatatgcttt aggacgtgtt gaagttacag 5641 caaaaaaatg gtatacactc acgttaacta ttaagggtca tttcgcctct ggcatgctga 5701 atgacaagtc tctgtggaca gacatccctg tgaattttcc aaagaatggc tgggctgcaa 5761 ttggaactca ctcctttgaa tttgcacagt ttgacaactt tcttgtggaa gccacacgct 5821 aaCCAGCTTT CTTGTACAAA GTGGTTGATA GCTTGGATCC AATCAACCTC TGGATTACAA 5881 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 5941 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 6001 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 6061 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 6121 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 6181 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 6241 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 6301 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 6361 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG 6421 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 6481 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 6541 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 6601 GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT 6661 AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT 6721 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC 6781 AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG 6841 CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT 6901 AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 6961 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 7021 TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 7081 GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 7141 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 7201 AATTTTAACA AAATATTAAC GCTTACAATT TAG //