LOCUS GCS1-pGEn1-DEST 6913 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5541..6082 /note="WPRE" misc_recomb 5503..5525 /label=attB2 /note="attB2 site" misc_feature complement(6089..6303) /note="BGH pA" misc_feature complement(6391..6520) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5502 /dnas_title="GCS1-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:34915 IMAGE:5110525" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3166..5502 /dnas_title="GCS1" /gene="GCS1" /db_xref="GeneID:7841" /db_xref="MIM:601336" CDS 3166..5502 /dnas_title="glucosidase I" /gene="GCS1" /codon_start=1 /product="glucosidase I" /protein_id="AAH28337.1" /db_xref="GI:62020522" /db_xref="GeneID:7841" /db_xref="MIM:601336" /translation="MARGERRRRAVPAEGVRTAERAARGGPGRRDGRGGGPRSTAGGV ALAVVVLSLALGMSGRWVLAWYRARRAVTLHSAPPVLPADSSSPAVAPDLFWGTYRPH VYFGMKTRSPKPLLTGLMWAQQGTTPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQH IQDGALRLTTEFVKRPGGQHGGDWSWRVTVEPQDSGTSALPLVSLFFYVVTDGKEVLL PEVGAKGQLKFISGHTSELGNFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEM VKSRLNSWFQHRPPGASPERYLGLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEF VFESGSAQAGGNQALPRLAGSLLTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQ AALSGLLGGIGYFYGQGLVLPDIGVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLW DEGFHQLVVQRWDPSLTREALGHWLGLLNADGWIGREQILGDEARARVPPEFLVQRAV HANPPTLLLPVAHMLEVGDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRD PALPTLLNPKTLPSGLDDYPRASHPSVTERHLDLRCWVALGARVLTRLAEHLGEAEVA AELGPLAASLEAAESLDELHWAPELGVFADFGNHTKAVQLKPRPPQGLVRVVGRPQPQ LQYVDALGYVSLFPLLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQ RNSEHDPPYWRGAVWLNVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQ YQATGFLWEQYSDRDGRGMGCRPFHGWTSLVLLAMAEDY" misc_feature 3166..5502 /note="GCS1 coding region" misc_difference 3703..3703 /dnas_title="GCS1" /gene="GCS1" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'N' in cDNA, 'D' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3865..3865 /dnas_title="GCS1" /gene="GCS1" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'S' in cDNA, 'P' in the human genome." vector join(5511..6913,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5510 /source="GCS1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggccgctg ggtgctggcg 3181 tggtaccgtg cgcggcgggc ggtcacgctg cactccgcgc ctcctgtgtt gcctgccgac 3241 tcctccagcc ccgccgtggc cccggacctc ttctggggaa cctaccgccc tcacgtctac 3301 ttcggcatga agacccgcag cccgaagccc ctcctcaccg gactgatgtg ggcgcagcag 3361 ggcaccaccc cggggactcc taagctcagg cacacgtgtg agcaggggga cggtgtgggt 3421 ccctatggct gggagttcca cgacggcctc tccttcgggc gccaacacat ccaggatggg 3481 gccttaaggc tcaccactga gttcgtcaag aggcctgggg gtcagcacgg aggggactgg 3541 agctggagag tgactgtaga gcctcaggac tcaggtactt ctgccctccc tttggtctcc 3601 ctgttcttct atgtggtgac agatggcaag gaagtcctac taccagaggt tggggccaag 3661 gggcagttga agtttatcag tgggcacacc agtgaacttg gtaacttccg ctttacactt 3721 ttgccaccaa ccagtccagg ggatacagcc cccaagtatg gcagctacaa tgtcttctgg 3781 acctccaacc caggactgcc cctgctgaca gagatggtaa agagtcgcct aaatagctgg 3841 tttcagcatc ggcccccagg ggcctcccct gaacgctacc tcggcttgcc aggatccctg 3901 aagtgggagg acagaggtcc aagtgggcaa gggcaggggc agttcttgat acagcaggtg 3961 accctgaaaa ttcccatttc catagagttt gtgtttgaat caggcagtgc ccaggcagga 4021 ggaaatcaag ccctgccaag actggcaggc agtctactga cccaggccct ggagagccat 4081 gctgaaggct ttagagagcg ctttgagaag accttccagc tgaaggagaa gggcctgagc 4141 tctggcgagc aggttttggg tcaggctgcc ctcagcggcc tccttggtgg aattggctac 4201 ttctacggac aagggctggt attgccagac atcggggtgg aagggtctga gcagaaggtg 4261 gacccagccc tctttccacc cgtacctctt tttacagcag tgccctcccg gtcattcttc 4321 ccacgaggct tcctttggga tgaaggcttt caccagctgg tggttcagcg gtgggatccc 4381 tccctcaccc gggaagccct tggccactgg ctggggctgc taaatgctga tggctggatt 4441 gggagggagc agatactggg ggatgaggcc cgagcccggg tgcctccaga attcctagta 4501 caacgagcag tccacgccaa ccccccaacc ctacttttgc ctgtagccca tatgctagag 4561 gttggtgacc ctgacgactt ggctttcctc cgaaaggcct tgccccgcct gcatgcctgg 4621 ttttcctggc tccatcagag ccaggcaggc ccactgccac tatcttaccg ctggcgggga 4681 cgggaccctg ccttaccaac cttactgaac cccaagaccc taccctctgg gctggatgac 4741 tacccccggg cttcacaccc ttcagtaacc gagcggcacc tggacctgcg atgttgggtg 4801 gcactgggtg cccgtgtgct gacgcggctg gcagagcatc tgggtgaggc tgaggtagct 4861 gctgagctgg gcccactggc tgcctcactg gaggcagcag agagcctgga tgagctgcac 4921 tgggccccag agctaggagt ctttgcagac tttgggaacc acacaaaagc agtacagctg 4981 aagcccaggc cccctcaggg gctcgttcgg gtggtgggtc ggccccaacc tcaactgcag 5041 tatgtagatg ctcttggcta tgtcagtctt tttcccttgc tgctgcgact gctggacccc 5101 acctcatccc gccttgggcc cctgctggac attctagccg acagccgcca tctctggagc 5161 ccctttggtt tacgctccct tgcagcctcc agctcctttt atggccagcg caattcagag 5221 catgatcccc cctactggcg gggtgctgtg tggctcaatg tcaactacct ggctttggga 5281 gcactccacc actatgggca tctggagggt cctcaccagg ctcgggctgc caaactccac 5341 ggtgagctcc gtgccaacgt ggtaggcaat gtatggcgcc agtaccaggc tacaggcttt 5401 ctttgggagc agtacagtga ccgcgatggg cgaggcatgg gctgccgccc tttccacggc 5461 tggaccagcc ttgtcttact ggccatggct gaagactact gaCCAGCTTT CTTGTACAAA 5521 GTGGTTGATA GCTTGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 5581 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 5641 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 5701 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5761 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5821 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5881 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5941 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 6001 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 6061 TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 6121 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 6181 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 6241 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 6301 GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA 6361 AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC 6421 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 6481 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG 6541 CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC 6601 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 6661 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 6721 AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC 6781 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 6841 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 6901 GCTTACAATT TAG //