LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2324..2420) /label=attL2 /note="attL2 (c)" misc_signal complement(2435..2454) /note="T7 Promoter/priming site (c)" primer_bind 2462..2478 /note="M13 Reverse priming site" gene 2591..3400 /note="Kanamycin resistance gene" rep_origin 3521..4194 /note="pUC origin" PCR_frame 669..2324 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2344) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2341) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2332..4197,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2302..2322 /note="TEV site" source 673..2301 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:59678 IMAGE:6250265" /tissue_type="Pancreas, ductal carcinoma" /clone_lib="NIH_MGC_110" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2301 /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" CDS 673..2301 /dnas_title="heparanase" /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /codon_start=1 /product="heparanase" /protein_id="AAH51321.1" /db_xref="GI:30410968" /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" /translation="MLLRSKPALPPPLMLLLLGPLGPLSPGALPRPAQAQDVVDLDFF TQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFL IFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKF KNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNI SWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTA KMLKSFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTR PGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYH LVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDL TLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTL PPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI" misc_feature 673..2301 /note="HPSE coding region" misc_difference 1592..1592 /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'R' in cDNA, 'K' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgctgct gcgctcgaag cctgcgctgc cgccgccgct gatgctgctg 721 ctcctggggc cgctgggtcc cctctcccct ggcgccctgc cccgacctgc gcaagcacag 781 gacgtcgtgg acctggactt cttcacccag gagccgctgc acctggtgag cccctcgttc 841 ctgtccgtca ccattgacgc caacctggcc acggacccgc ggttcctcat cctcctgggt 901 tctccaaagc ttcgtacctt ggccagaggc ttgtctcctg cgtacctgag gtttggtggc 961 accaagacag acttcctaat tttcgatccc aagaaggaat caacctttga agagagaagt 1021 tactggcaat ctcaagtcaa ccaggatatt tgcaaatatg gatccatccc tcctgatgtg 1081 gaggagaagt tacggttgga atggccctac caggagcaat tgctactccg agaacactac 1141 cagaaaaagt tcaagaacag cacctactca agaagctctg tagatgtgct atacactttt 1201 gcaaactgct caggactgga cttgatcttt ggcctaaatg cgttattaag aacagcagat 1261 ttgcagtgga acagttctaa tgctcagttg ctcctggact actgctcttc caaggggtat 1321 aacatttctt gggaactagg caatgaacct aacagtttcc ttaagaaggc tgatattttc 1381 atcaatgggt cgcagttagg agaagatttt attcaattgc ataaacttct aagaaagtcc 1441 accttcaaaa atgcaaaact ctatggtcct gatgttggtc agcctcgaag aaagacggct 1501 aagatgctga agagcttcct gaaggctggt ggagaagtga ttgattcagt tacatggcat 1561 cactactatt tgaatggacg gactgctacc agggaagatt ttctaaaccc tgatgtattg 1621 gacattttta tttcatctgt gcaaaaagtt ttccaggtgg ttgagagcac caggcctggc 1681 aagaaggtct ggttaggaga aacaagctct gcatatggag gcggagcgcc cttgctatcc 1741 gacacctttg cagctggctt tatgtggctg gataaattgg gcctgtcagc ccgaatggga 1801 atagaagtgg tgatgaggca agtattcttt ggagcaggaa actaccattt agtggatgaa 1861 aacttcgatc ctttacctga ttattggcta tctcttctgt tcaagaaatt ggtgggcacc 1921 aaggtgttaa tggcaagcgt gcaaggttca aagagaagga agcttcgagt ataccttcat 1981 tgcacaaaca ctgacaatcc aaggtataaa gaaggagatt taactctgta tgccataaac 2041 ctccataatg tcaccaagta cttgcggtta ccctatcctt tttctaacaa gcaagtggat 2101 aaataccttc taagaccttt gggacctcat ggattacttt ccaaatctgt ccaactcaat 2161 ggtctaactc taaagatggt ggatgatcaa accttgccac ctttaatgga aaaacctctc 2221 cggccaggaa gttcactggg cttgccagct ttctcatata gtttttttgt gataagaaat 2281 gccaaagttg ctgcttgcat cgaaaacttg tactttcaag gctCCAGCTT TCTTGTACAA 2341 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 2401 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 2461 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 2521 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 2581 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 2641 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 2701 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 2761 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 2821 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 2881 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 2941 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 3001 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 3061 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 3121 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 3181 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 3241 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 3301 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 3361 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 3421 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 3481 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3541 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3601 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3661 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 3721 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3781 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3841 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3901 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 3961 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4021 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4081 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 4141 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //