LOCUS HPSE-pGEc2-DEST 6850 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6328..6457) /note="3'-ITR" misc_feature complement(6026..6240) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5478..6019 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4657..4679 /note="attB2" misc_feature 5457..5462 /note="BstB1 site" misc_feature 4680..5375 /note="Super GFP" misc_feature 5376..5426 /note="AviTag" misc_feature 5427..5450 /note="His Tag" PCR_frame 3002..4657 /note="Reading Frame" /set="Set 1" source 3006..4634 /dnas_title="HPSE-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:59678 IMAGE:6250265" /tissue_type="Pancreas, ductal carcinoma" /clone_lib="NIH_MGC_110" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4634 /dnas_title="HPSE" /gene="HPSE" /gene_synonym="HPA" /gene_synonym="HPR1" /gene_synonym="HPSE1" /gene_synonym="HSE1" /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" CDS 3006..4634 /dnas_title="heparanase" /gene="HPSE" /gene_synonym="HPA" /gene_synonym="HPR1" /gene_synonym="HPSE1" /gene_synonym="HSE1" /codon_start=1 /product="heparanase" /protein_id="AAH51321.1" /db_xref="GI:30410968" /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" /translation="MLLRSKPALPPPLMLLLLGPLGPLSPGALPRPAQAQDVVDLDFF TQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFL IFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKF KNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNI SWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTA KMLKSFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTR PGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYH LVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDL TLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTL PPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI" misc_feature 3006..4634 /note="HPSE coding region" misc_difference 3925..3925 /dnas_title="HPSE" /gene="HPSE" /gene_synonym="HPA" /gene_synonym="HPR1" /gene_synonym="HPSE1" /gene_synonym="HSE1" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'R' in cDNA, 'K' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4635..4655 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4664) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4664) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4665..6850,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4664 /source="HPSE-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgct gctgcgctcg aagcctgcgc tgccgccgcc gctgatgctg ctgctcctgg 3061 ggccgctggg tcccctctcc cctggcgccc tgccccgacc tgcgcaagca caggacgtcg 3121 tggacctgga cttcttcacc caggagccgc tgcacctggt gagcccctcg ttcctgtccg 3181 tcaccattga cgccaacctg gccacggacc cgcggttcct catcctcctg ggttctccaa 3241 agcttcgtac cttggccaga ggcttgtctc ctgcgtacct gaggtttggt ggcaccaaga 3301 cagacttcct aattttcgat cccaagaagg aatcaacctt tgaagagaga agttactggc 3361 aatctcaagt caaccaggat atttgcaaat atggatccat ccctcctgat gtggaggaga 3421 agttacggtt ggaatggccc taccaggagc aattgctact ccgagaacac taccagaaaa 3481 agttcaagaa cagcacctac tcaagaagct ctgtagatgt gctatacact tttgcaaact 3541 gctcaggact ggacttgatc tttggcctaa atgcgttatt aagaacagca gatttgcagt 3601 ggaacagttc taatgctcag ttgctcctgg actactgctc ttccaagggg tataacattt 3661 cttgggaact aggcaatgaa cctaacagtt tccttaagaa ggctgatatt ttcatcaatg 3721 ggtcgcagtt aggagaagat tttattcaat tgcataaact tctaagaaag tccaccttca 3781 aaaatgcaaa actctatggt cctgatgttg gtcagcctcg aagaaagacg gctaagatgc 3841 tgaagagctt cctgaaggct ggtggagaag tgattgattc agttacatgg catcactact 3901 atttgaatgg acggactgct accagggaag attttctaaa ccctgatgta ttggacattt 3961 ttatttcatc tgtgcaaaaa gttttccagg tggttgagag caccaggcct ggcaagaagg 4021 tctggttagg agaaacaagc tctgcatatg gaggcggagc gcccttgcta tccgacacct 4081 ttgcagctgg ctttatgtgg ctggataaat tgggcctgtc agcccgaatg ggaatagaag 4141 tggtgatgag gcaagtattc tttggagcag gaaactacca tttagtggat gaaaacttcg 4201 atcctttacc tgattattgg ctatctcttc tgttcaagaa attggtgggc accaaggtgt 4261 taatggcaag cgtgcaaggt tcaaagagaa ggaagcttcg agtatacctt cattgcacaa 4321 acactgacaa tccaaggtat aaagaaggag atttaactct gtatgccata aacctccata 4381 atgtcaccaa gtacttgcgg ttaccctatc ctttttctaa caagcaagtg gataaatacc 4441 ttctaagacc tttgggacct catggattac tttccaaatc tgtccaactc aatggtctaa 4501 ctctaaagat ggtggatgat caaaccttgc cacctttaat ggaaaaacct ctccggccag 4561 gaagttcact gggcttgcca gctttctcat atagtttttt tgtgataaga aatgccaaag 4621 ttgctgcttg catcgaaaac ttgtactttc aaggctCCAG CTTTCTTGTA CAAAGTGGTA 4681 TGAGCAAGGG CGAGGAACTG TTCACCGGCG TGGTGCCCAT CCTGGTGGAA CTGGATGGCG 4741 ACGTGAACGG CCACAAGTTC TCTGTGCGGG GAGAGGGCGA AGGCGACGCC ACAAATGGCA 4801 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCTTGG CCTACCCTCG 4861 TGACCACACT GACCTACGGC GTGCAGTGCT TCAGCAGATA CCCCGACCAT ATGAAGCGGC 4921 ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACGTGCA GGAACGGACC ATCAGCTTCA 4981 AGGACGACGG CACCTACAAG ACCAGAGCCG AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA 5041 ACCGGATCGA GCTGAAGGGC ATCGATTTCA AAGAGGACGG CAACATCCTG GGCCACAAGC 5101 TGGAGTACAA CTTCAACAGC CACAACGTGT ACATCACCGC CGACAAGCAG AAGAACGGCA 5161 TCAAGGCCAA CTTCAAGATC CGGCACAACG TGGAAGATGG CAGCGTGCAG CTGGCCGACC 5221 ACTACCAGCA GAACACCCCC ATCGGAGATG GCCCCGTGCT GCTGCCCGAC AACCACTACC 5281 TGAGCACCCA GAGCGTGCTG AGCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC 5341 TGGAATTCGT GACCGCCGCT GGCATCACCC ACGGCATGAG CGGCCTGAAC GACATCTTCG 5401 AGGCCCAGAA AATCGAGTGG CACGAGCACC ACCATCATCA CCATCACCAC TGATGATTCG 5461 AAAAATAATA AGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 5521 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 5581 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 5641 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 5701 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 5761 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 5821 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT 5881 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 5941 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 6001 GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 6061 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 6121 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 6181 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 6241 CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT 6301 AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 6361 CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT 6421 TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC 6481 GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT 6541 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 6601 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA 6661 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT 6721 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 6781 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 6841 TACAATTTAG //