LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2219..2315) /label=attL2 /note="attL2 (c)" misc_signal complement(2330..2349) /note="T7 Promoter/priming site (c)" primer_bind 2357..2373 /note="M13 Reverse priming site" gene 2486..3295 /note="Kanamycin resistance gene" rep_origin 3416..4089 /note="pUC origin" vector join(2227..4092,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2218 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:59678 IMAGE:6250265" /tissue_type="Pancreas, ductal carcinoma" /clone_lib="NIH_MGC_110" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2218 /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" CDS 692..2218 /dnas_title="heparanase" /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /codon_start=1 /product="heparanase" /protein_id="AAH51321.1" /db_xref="GI:30410968" /db_xref="GeneID:10855" /db_xref="HGNC:5164" /db_xref="MIM:604724" /translation="MLLRSKPALPPPLMLLLLGPLGPLSPGALPRPAQAQDVVDLDFF TQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFL IFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKF KNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNI SWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTA KMLKSFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTR PGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYH LVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDL TLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTL PPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI" misc_feature 692..2218 /note="HPSE coding region" misc_difference 1506..1506 /gene="HPSE" /gene_synonym=HPA /gene_synonym=HPR1 /gene_synonym=HPSE1 /gene_synonym=HSE1 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'R' in cDNA, 'K' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccaggacgtc gtggacctgg acttcttcac 721 ccaggagccg ctgcacctgg tgagcccctc gttcctgtcc gtcaccattg acgccaacct 781 ggccacggac ccgcggttcc tcatcctcct gggttctcca aagcttcgta ccttggccag 841 aggcttgtct cctgcgtacc tgaggtttgg tggcaccaag acagacttcc taattttcga 901 tcccaagaag gaatcaacct ttgaagagag aagttactgg caatctcaag tcaaccagga 961 tatttgcaaa tatggatcca tccctcctga tgtggaggag aagttacggt tggaatggcc 1021 ctaccaggag caattgctac tccgagaaca ctaccagaaa aagttcaaga acagcaccta 1081 ctcaagaagc tctgtagatg tgctatacac ttttgcaaac tgctcaggac tggacttgat 1141 ctttggccta aatgcgttat taagaacagc agatttgcag tggaacagtt ctaatgctca 1201 gttgctcctg gactactgct cttccaaggg gtataacatt tcttgggaac taggcaatga 1261 acctaacagt ttccttaaga aggctgatat tttcatcaat gggtcgcagt taggagaaga 1321 ttttattcaa ttgcataaac ttctaagaaa gtccaccttc aaaaatgcaa aactctatgg 1381 tcctgatgtt ggtcagcctc gaagaaagac ggctaagatg ctgaagagct tcctgaaggc 1441 tggtggagaa gtgattgatt cagttacatg gcatcactac tatttgaatg gacggactgc 1501 taccagggaa gattttctaa accctgatgt attggacatt tttatttcat ctgtgcaaaa 1561 agttttccag gtggttgaga gcaccaggcc tggcaagaag gtctggttag gagaaacaag 1621 ctctgcatat ggaggcggag cgcccttgct atccgacacc tttgcagctg gctttatgtg 1681 gctggataaa ttgggcctgt cagcccgaat gggaatagaa gtggtgatga ggcaagtatt 1741 ctttggagca ggaaactacc atttagtgga tgaaaacttc gatcctttac ctgattattg 1801 gctatctctt ctgttcaaga aattggtggg caccaaggtg ttaatggcaa gcgtgcaagg 1861 ttcaaagaga aggaagcttc gagtatacct tcattgcaca aacactgaca atccaaggta 1921 taaagaagga gatttaactc tgtatgccat aaacctccat aatgtcacca agtacttgcg 1981 gttaccctat cctttttcta acaagcaagt ggataaatac cttctaagac ctttgggacc 2041 tcatggatta ctttccaaat ctgtccaact caatggtcta actctaaaga tggtggatga 2101 tcaaaccttg ccacctttaa tggaaaaacc tctccggcca ggaagttcac tgggcttgcc 2161 agctttctca tatagttttt ttgtgataag aaatgccaaa gttgctgctt gcatctgaCC 2221 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 2281 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 2341 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 2401 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 2461 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 2521 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 2581 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2641 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2701 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2761 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2821 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2881 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2941 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 3001 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 3061 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 3121 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 3181 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 3241 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 3301 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 3361 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 3421 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3481 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3541 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3601 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3661 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3721 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3781 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3841 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3901 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3961 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4021 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4081 TGCTCACATG TT //