LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2471..2567) /label=attL2 /note="attL2 (c)" misc_signal complement(2582..2601) /note="T7 Promoter/priming site (c)" primer_bind 2609..2625 /note="M13 Reverse priming site" gene 2738..3547 /note="Kanamycin resistance gene" rep_origin 3668..4341 /note="pUC origin" PCR_frame 669..2471 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2491) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2488) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2479..4344,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2449..2469 /note="TEV site" source 673..2448 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:133234 IMAGE:40033830" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: AGGAGAAG" gene 673..2448 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /db_xref="GeneID:60495" /db_xref="HGNC:18374" CDS 673..2448 /dnas_title="heparanase 2" /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /codon_start=1 /product="heparanase 2" /protein_id="AAI12357.1" /db_xref="GI:85662624" /db_xref="GeneID:60495" /db_xref="HGNC:18374" /translation="MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDR RPLPVDRAAGLKEKTLILLDVSTKNPVRTVNENFLSLQLDPSIIHDGWLDFLSSKRLV TLARGLSPAFLRFGGKRTDFLQFQNLRNPAKSRGGPGPDYYLKNYEDDIVRSDVALDK QKGCKIAQHPDVMLELQREKAAQMHLVLLKEQFSNTYSNLILTARSLDKLYNFADCSG LHLIFALNALRRNPNNSWNSSSALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNG SQLGKDYIQLKSLLQPIRIYSRASLYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQ HCYIDGRVVKVMDFLKTRLLDTLSDQIRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNN LSDSYAAGFLWLNTLGMLANQGIDVVIRHSFFDHGYNHLVDQNFNPLPDYWLSLLYKR LIGPKVLAVHVAGLQRKPRPGRVIRDKLRIYAHCTNHHNHNYVRGSITLFIINLHRSR KKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSVQLNGQPLVMVDDGTLPELKPRPLRAG RTLVIPPVTMGFFVVKNVNALACRYR" misc_feature 673..2448 /note="HPSE2 coding region" misc_difference 2212..2212 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2408..2408 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'F' in cDNA, 'Y' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgagggt gctttgtgcc ttccctgaag ccatgccctc cagcaactcc 721 cgcccccccg cgtgcctagc cccgggggct ctctacttgg ctctgttgct ccatctctcc 781 ctttcctccc aggctggaga caggagaccc ttgcctgtag acagagctgc aggtttgaag 841 gaaaagaccc tgattctact tgatgtgagc accaagaacc cagtcaggac agtcaatgag 901 aacttcctct ctctgcagct ggatccgtcc atcattcatg atggctggct cgatttccta 961 agctccaagc gcttggtgac cctggcccgg ggactttcgc ccgcctttct gcgcttcggg 1021 ggcaaaagga ccgacttcct gcagttccag aacctgagga acccggcgaa aagccgcggg 1081 ggcccgggcc cggattacta tctcaaaaac tatgaggatg acattgttcg aagtgatgtt 1141 gccttagata aacagaaagg ctgcaagatt gcccagcacc ctgatgttat gctggagctc 1201 caaagggaga aggcagctca gatgcatctg gttcttctaa aggagcaatt ctccaatact 1261 tacagtaatc tcatattaac agccaggtct ctagacaaac tttataactt tgctgattgc 1321 tctggactcc acctgatatt tgctctaaat gcactgcgtc gtaatcccaa taactcctgg 1381 aacagttcta gtgccctgag tctgttgaag tacagcgcca gcaaaaagta caacatttct 1441 tgggaactgg gtaatgagcc aaataactat cggaccatgc atggccgggc agtaaatggc 1501 agccagttgg gaaaggatta catccagctg aagagcctgt tgcagcccat ccggatttat 1561 tccagagcca gcttatatgg ccctaatatt gggcggccga ggaagaatgt catcgccctc 1621 ctagatggat tcatgaaggt ggcaggaagt acagtagatg cagttacctg gcaacattgc 1681 tacattgatg gccgggtggt caaggtgatg gacttcctga aaactcgcct gttagacaca 1741 ctctctgacc agattaggaa aattcagaaa gtggttaata catacactcc aggaaagaag 1801 atttggcttg aaggtgtggt gaccacctca gctggaggca caaacaatct atccgattcc 1861 tatgctgcag gattcttatg gttgaacact ttaggaatgc tggccaatca gggcattgat 1921 gtcgtgatac ggcactcatt ttttgaccat ggatacaatc acctcgtgga ccagaatttt 1981 aacccattac cagactactg gctctctctc ctctacaagc gcctgatcgg ccccaaagtc 2041 ttggctgtgc atgtggctgg gctccagcgg aagccacggc ctggccgagt gatccgggac 2101 aaactaagga tttatgctca ctgcacaaac caccacaacc acaactacgt tcgtgggtcc 2161 attacacttt ttatcatcaa cttgcatcga tcaagaaaga aaatcaagct gactgggact 2221 ctcagagaca agctggttca ccagtacctg ctgcagccct atgggcagga gggcctaaag 2281 tccaagtcag tgcaactgaa tggccagccc ttagtgatgg tggacgacgg gaccctccca 2341 gaattgaagc cccgccccct tcgggccggc cggacattgg tcatccctcc agtcaccatg 2401 ggcttttttg tggtcaagaa tgtcaatgct ttggcctgcc gctaccgaga aaacttgtac 2461 tttcaaggct CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 2521 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA 2581 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT 2641 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA 2701 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC 2761 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG 2821 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC 2881 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT 2941 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC 3001 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT 3061 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG 3121 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC 3181 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG 3241 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC 3301 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 3361 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC 3421 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA 3481 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT 3541 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG 3601 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC 3661 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3721 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3781 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3841 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3901 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3961 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 4021 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 4081 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 4141 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 4201 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 4261 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4321 TTGCTGGCCT TTTGCTCACA TGTT //