LOCUS HPSE2-pGEc1-DEST 6271 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5749..5878) /note="3'-ITR" misc_feature complement(5447..5661) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4899..5440 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4804..4826 /label=attB2 /note="attB2 site" misc_feature 4836..4859 /note="8xHis Tag" misc_feature 4863..4886 /note="StrepTag" PCR_frame 3002..4804 /note="Reading Frame" /set="Set 1" source 3006..4781 /dnas_title="HPSE2-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:133234 IMAGE:40033830" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: AGGAGAAG" gene 3006..4781 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /db_xref="GeneID:60495" /db_xref="HGNC:18374" CDS 3006..4781 /dnas_title="heparanase 2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /codon_start=1 /product="heparanase 2" /protein_id="AAI12357.1" /db_xref="GI:85662624" /db_xref="GeneID:60495" /db_xref="HGNC:18374" /translation="MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDR RPLPVDRAAGLKEKTLILLDVSTKNPVRTVNENFLSLQLDPSIIHDGWLDFLSSKRLV TLARGLSPAFLRFGGKRTDFLQFQNLRNPAKSRGGPGPDYYLKNYEDDIVRSDVALDK QKGCKIAQHPDVMLELQREKAAQMHLVLLKEQFSNTYSNLILTARSLDKLYNFADCSG LHLIFALNALRRNPNNSWNSSSALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNG SQLGKDYIQLKSLLQPIRIYSRASLYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQ HCYIDGRVVKVMDFLKTRLLDTLSDQIRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNN LSDSYAAGFLWLNTLGMLANQGIDVVIRHSFFDHGYNHLVDQNFNPLPDYWLSLLYKR LIGPKVLAVHVAGLQRKPRPGRVIRDKLRIYAHCTNHHNHNYVRGSITLFIINLHRSR KKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSVQLNGQPLVMVDDGTLPELKPRPLRAG RTLVIPPVTMGFFVVKNVNALACRYR" misc_feature 3006..4781 /note="HPSE2 coding region" misc_feature 4782..4802 /note="TEV site" misc_difference 4545..4545 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4741..4741 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'F' in cDNA, 'Y' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4811) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4811) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4812..6271,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4811 /source="HPSE2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag ggtgctttgt gccttccctg aagccatgcc ctccagcaac tcccgccccc 3061 ccgcgtgcct agccccgggg gctctctact tggctctgtt gctccatctc tccctttcct 3121 cccaggctgg agacaggaga cccttgcctg tagacagagc tgcaggtttg aaggaaaaga 3181 ccctgattct acttgatgtg agcaccaaga acccagtcag gacagtcaat gagaacttcc 3241 tctctctgca gctggatccg tccatcattc atgatggctg gctcgatttc ctaagctcca 3301 agcgcttggt gaccctggcc cggggacttt cgcccgcctt tctgcgcttc gggggcaaaa 3361 ggaccgactt cctgcagttc cagaacctga ggaacccggc gaaaagccgc gggggcccgg 3421 gcccggatta ctatctcaaa aactatgagg atgacattgt tcgaagtgat gttgccttag 3481 ataaacagaa aggctgcaag attgcccagc accctgatgt tatgctggag ctccaaaggg 3541 agaaggcagc tcagatgcat ctggttcttc taaaggagca attctccaat acttacagta 3601 atctcatatt aacagccagg tctctagaca aactttataa ctttgctgat tgctctggac 3661 tccacctgat atttgctcta aatgcactgc gtcgtaatcc caataactcc tggaacagtt 3721 ctagtgccct gagtctgttg aagtacagcg ccagcaaaaa gtacaacatt tcttgggaac 3781 tgggtaatga gccaaataac tatcggacca tgcatggccg ggcagtaaat ggcagccagt 3841 tgggaaagga ttacatccag ctgaagagcc tgttgcagcc catccggatt tattccagag 3901 ccagcttata tggccctaat attgggcggc cgaggaagaa tgtcatcgcc ctcctagatg 3961 gattcatgaa ggtggcagga agtacagtag atgcagttac ctggcaacat tgctacattg 4021 atggccgggt ggtcaaggtg atggacttcc tgaaaactcg cctgttagac acactctctg 4081 accagattag gaaaattcag aaagtggtta atacatacac tccaggaaag aagatttggc 4141 ttgaaggtgt ggtgaccacc tcagctggag gcacaaacaa tctatccgat tcctatgctg 4201 caggattctt atggttgaac actttaggaa tgctggccaa tcagggcatt gatgtcgtga 4261 tacggcactc attttttgac catggataca atcacctcgt ggaccagaat tttaacccat 4321 taccagacta ctggctctct ctcctctaca agcgcctgat cggccccaaa gtcttggctg 4381 tgcatgtggc tgggctccag cggaagccac ggcctggccg agtgatccgg gacaaactaa 4441 ggatttatgc tcactgcaca aaccaccaca accacaacta cgttcgtggg tccattacac 4501 tttttatcat caacttgcat cgatcaagaa agaaaatcaa gctgactggg actctcagag 4561 acaagctggt tcaccagtac ctgctgcagc cctatgggca ggagggccta aagtccaagt 4621 cagtgcaact gaatggccag cccttagtga tggtggacga cgggaccctc ccagaattga 4681 agccccgccc ccttcgggcc ggccggacat tggtcatccc tccagtcacc atgggctttt 4741 ttgtggtcaa gaatgtcaat gctttggcct gccgctaccg agaaaacttg tactttcaag 4801 gctCCAGCTT TCTTGTACAA AGTGGTGATG GCCGCCATCA TCATCATCAT CATCATCATG 4861 CGTGGAGCCA CCCGCAGTTC GAAAAATAAT AAGGATCCAA TCAACCTCTG GATTACAAAA 4921 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 4981 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 5041 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 5101 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 5161 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 5221 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 5281 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 5341 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 5401 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 5461 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 5521 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 5581 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 5641 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 5701 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 5761 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 5821 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 5881 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 5941 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 6001 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 6061 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 6121 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 6181 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 6241 TTTTAACAAA ATATTAACGC TTACAATTTA G //