LOCUS HPSE2-pGEc2-DEST 6997 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6475..6604) /note="3'-ITR" misc_feature complement(6173..6387) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5625..6166 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4804..4826 /note="attB2" misc_feature 5604..5609 /note="BstB1 site" misc_feature 4827..5522 /note="Super GFP" misc_feature 5523..5573 /note="AviTag" misc_feature 5574..5597 /note="His Tag" PCR_frame 3002..4804 /note="Reading Frame" /set="Set 1" source 3006..4781 /dnas_title="HPSE2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:133234 IMAGE:40033830" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: AGGAGAAG" gene 3006..4781 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /db_xref="GeneID:60495" /db_xref="HGNC:18374" CDS 3006..4781 /dnas_title="heparanase 2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /codon_start=1 /product="heparanase 2" /protein_id="AAI12357.1" /db_xref="GI:85662624" /db_xref="GeneID:60495" /db_xref="HGNC:18374" /translation="MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDR RPLPVDRAAGLKEKTLILLDVSTKNPVRTVNENFLSLQLDPSIIHDGWLDFLSSKRLV TLARGLSPAFLRFGGKRTDFLQFQNLRNPAKSRGGPGPDYYLKNYEDDIVRSDVALDK QKGCKIAQHPDVMLELQREKAAQMHLVLLKEQFSNTYSNLILTARSLDKLYNFADCSG LHLIFALNALRRNPNNSWNSSSALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNG SQLGKDYIQLKSLLQPIRIYSRASLYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQ HCYIDGRVVKVMDFLKTRLLDTLSDQIRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNN LSDSYAAGFLWLNTLGMLANQGIDVVIRHSFFDHGYNHLVDQNFNPLPDYWLSLLYKR LIGPKVLAVHVAGLQRKPRPGRVIRDKLRIYAHCTNHHNHNYVRGSITLFIINLHRSR KKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSVQLNGQPLVMVDDGTLPELKPRPLRAG RTLVIPPVTMGFFVVKNVNALACRYR" misc_feature 3006..4781 /note="HPSE2 coding region" misc_feature 4782..4802 /note="TEV site" misc_difference 4545..4545 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4741..4741 /dnas_title="HPSE2" /gene="HPSE2" /gene_synonym="HPA2" /gene_synonym="HPR2" /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'F' in cDNA, 'Y' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4811) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4811) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4812..6997,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4811 /source="HPSE2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag ggtgctttgt gccttccctg aagccatgcc ctccagcaac tcccgccccc 3061 ccgcgtgcct agccccgggg gctctctact tggctctgtt gctccatctc tccctttcct 3121 cccaggctgg agacaggaga cccttgcctg tagacagagc tgcaggtttg aaggaaaaga 3181 ccctgattct acttgatgtg agcaccaaga acccagtcag gacagtcaat gagaacttcc 3241 tctctctgca gctggatccg tccatcattc atgatggctg gctcgatttc ctaagctcca 3301 agcgcttggt gaccctggcc cggggacttt cgcccgcctt tctgcgcttc gggggcaaaa 3361 ggaccgactt cctgcagttc cagaacctga ggaacccggc gaaaagccgc gggggcccgg 3421 gcccggatta ctatctcaaa aactatgagg atgacattgt tcgaagtgat gttgccttag 3481 ataaacagaa aggctgcaag attgcccagc accctgatgt tatgctggag ctccaaaggg 3541 agaaggcagc tcagatgcat ctggttcttc taaaggagca attctccaat acttacagta 3601 atctcatatt aacagccagg tctctagaca aactttataa ctttgctgat tgctctggac 3661 tccacctgat atttgctcta aatgcactgc gtcgtaatcc caataactcc tggaacagtt 3721 ctagtgccct gagtctgttg aagtacagcg ccagcaaaaa gtacaacatt tcttgggaac 3781 tgggtaatga gccaaataac tatcggacca tgcatggccg ggcagtaaat ggcagccagt 3841 tgggaaagga ttacatccag ctgaagagcc tgttgcagcc catccggatt tattccagag 3901 ccagcttata tggccctaat attgggcggc cgaggaagaa tgtcatcgcc ctcctagatg 3961 gattcatgaa ggtggcagga agtacagtag atgcagttac ctggcaacat tgctacattg 4021 atggccgggt ggtcaaggtg atggacttcc tgaaaactcg cctgttagac acactctctg 4081 accagattag gaaaattcag aaagtggtta atacatacac tccaggaaag aagatttggc 4141 ttgaaggtgt ggtgaccacc tcagctggag gcacaaacaa tctatccgat tcctatgctg 4201 caggattctt atggttgaac actttaggaa tgctggccaa tcagggcatt gatgtcgtga 4261 tacggcactc attttttgac catggataca atcacctcgt ggaccagaat tttaacccat 4321 taccagacta ctggctctct ctcctctaca agcgcctgat cggccccaaa gtcttggctg 4381 tgcatgtggc tgggctccag cggaagccac ggcctggccg agtgatccgg gacaaactaa 4441 ggatttatgc tcactgcaca aaccaccaca accacaacta cgttcgtggg tccattacac 4501 tttttatcat caacttgcat cgatcaagaa agaaaatcaa gctgactggg actctcagag 4561 acaagctggt tcaccagtac ctgctgcagc cctatgggca ggagggccta aagtccaagt 4621 cagtgcaact gaatggccag cccttagtga tggtggacga cgggaccctc ccagaattga 4681 agccccgccc ccttcgggcc ggccggacat tggtcatccc tccagtcacc atgggctttt 4741 ttgtggtcaa gaatgtcaat gctttggcct gccgctaccg agaaaacttg tactttcaag 4801 gctCCAGCTT TCTTGTACAA AGTGGTATGA GCAAGGGCGA GGAACTGTTC ACCGGCGTGG 4861 TGCCCATCCT GGTGGAACTG GATGGCGACG TGAACGGCCA CAAGTTCTCT GTGCGGGGAG 4921 AGGGCGAAGG CGACGCCACA AATGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 4981 AGCTGCCCGT GCCTTGGCCT ACCCTCGTGA CCACACTGAC CTACGGCGTG CAGTGCTTCA 5041 GCAGATACCC CGACCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT 5101 ACGTGCAGGA ACGGACCATC AGCTTCAAGG ACGACGGCAC CTACAAGACC AGAGCCGAAG 5161 TGAAGTTCGA GGGCGACACC CTCGTGAACC GGATCGAGCT GAAGGGCATC GATTTCAAAG 5221 AGGACGGCAA CATCCTGGGC CACAAGCTGG AGTACAACTT CAACAGCCAC AACGTGTACA 5281 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGG CACAACGTGG 5341 AAGATGGCAG CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC GGAGATGGCC 5401 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGAG CGTGCTGAGC AAGGACCCCA 5461 ACGAGAAGCG GGACCACATG GTGCTGCTGG AATTCGTGAC CGCCGCTGGC ATCACCCACG 5521 GCATGAGCGG CCTGAACGAC ATCTTCGAGG CCCAGAAAAT CGAGTGGCAC GAGCACCACC 5581 ATCATCACCA TCACCACTGA TGATTCGAAA AATAATAAGG ATCCAATCAA CCTCTGGATT 5641 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 5701 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 5761 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 5821 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 5881 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 5941 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 6001 CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT 6061 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 6121 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC 6181 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 6241 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 6301 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 6361 GGAAGACAAT AGCAGGCATG CTGGGGACTC GAGTTAAGGG CGAATTGCCG ATTAGGATCT 6421 TCCTAGAGCA TGGCTACGTA GATAAGTAGC ATGGCGGGTT AATCATTAAC TACAAGGAAC 6481 CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC 6541 GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC 6601 GCAGCCTTAA TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA CGGCCTTCCG CCAATTCGCC 6661 CTATAGTGAG TCGTATTACG TCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG 6721 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC 6781 GTAATAGCGA AGAGGCCCGC ACCGAAACGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 6841 GAATGGGAGC GCCCTGTAGC GGCCACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 6901 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 6961 CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAG //