LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2471..2567) /label=attL2 /note="attL2 (c)" misc_signal complement(2582..2601) /note="T7 Promoter/priming site (c)" primer_bind 2609..2625 /note="M13 Reverse priming site" gene 2738..3547 /note="Kanamycin resistance gene" rep_origin 3668..4341 /note="pUC origin" vector join(2479..4344,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2470 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:133234 IMAGE:40033830" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: AGGAGAAG" gene 692..2470 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /db_xref="GeneID:60495" /db_xref="HGNC:18374" CDS 692..2470 /dnas_title="heparanase 2" /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /codon_start=1 /product="heparanase 2" /protein_id="AAI12357.1" /db_xref="GI:85662624" /db_xref="GeneID:60495" /db_xref="HGNC:18374" /translation="MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDR RPLPVDRAAGLKEKTLILLDVSTKNPVRTVNENFLSLQLDPSIIHDGWLDFLSSKRLV TLARGLSPAFLRFGGKRTDFLQFQNLRNPAKSRGGPGPDYYLKNYEDDIVRSDVALDK QKGCKIAQHPDVMLELQREKAAQMHLVLLKEQFSNTYSNLILTARSLDKLYNFADCSG LHLIFALNALRRNPNNSWNSSSALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNG SQLGKDYIQLKSLLQPIRIYSRASLYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQ HCYIDGRVVKVMDFLKTRLLDTLSDQIRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNN LSDSYAAGFLWLNTLGMLANQGIDVVIRHSFFDHGYNHLVDQNFNPLPDYWLSLLYKR LIGPKVLAVHVAGLQRKPRPGRVIRDKLRIYAHCTNHHNHNYVRGSITLFIINLHRSR KKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSVQLNGQPLVMVDDGTLPELKPRPLRAG RTLVIPPVTMGFFVVKNVNALACRYR" misc_feature 692..2470 /note="HPSE2 coding region" misc_difference 2231..2231 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2427..2427 /gene="HPSE2" /gene_synonym=HPA2 /gene_synonym=HPR2 /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'F' in cDNA, 'Y' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catgagggtg ctttgtgcct tccctgaagc 721 catgccctcc agcaactccc gcccccccgc gtgcctagcc ccgggggctc tctacttggc 781 tctgttgctc catctctccc tttcctccca ggctggagac aggagaccct tgcctgtaga 841 cagagctgca ggtttgaagg aaaagaccct gattctactt gatgtgagca ccaagaaccc 901 agtcaggaca gtcaatgaga acttcctctc tctgcagctg gatccgtcca tcattcatga 961 tggctggctc gatttcctaa gctccaagcg cttggtgacc ctggcccggg gactttcgcc 1021 cgcctttctg cgcttcgggg gcaaaaggac cgacttcctg cagttccaga acctgaggaa 1081 cccggcgaaa agccgcgggg gcccgggccc ggattactat ctcaaaaact atgaggatga 1141 cattgttcga agtgatgttg ccttagataa acagaaaggc tgcaagattg cccagcaccc 1201 tgatgttatg ctggagctcc aaagggagaa ggcagctcag atgcatctgg ttcttctaaa 1261 ggagcaattc tccaatactt acagtaatct catattaaca gccaggtctc tagacaaact 1321 ttataacttt gctgattgct ctggactcca cctgatattt gctctaaatg cactgcgtcg 1381 taatcccaat aactcctgga acagttctag tgccctgagt ctgttgaagt acagcgccag 1441 caaaaagtac aacatttctt gggaactggg taatgagcca aataactatc ggaccatgca 1501 tggccgggca gtaaatggca gccagttggg aaaggattac atccagctga agagcctgtt 1561 gcagcccatc cggatttatt ccagagccag cttatatggc cctaatattg ggcggccgag 1621 gaagaatgtc atcgccctcc tagatggatt catgaaggtg gcaggaagta cagtagatgc 1681 agttacctgg caacattgct acattgatgg ccgggtggtc aaggtgatgg acttcctgaa 1741 aactcgcctg ttagacacac tctctgacca gattaggaaa attcagaaag tggttaatac 1801 atacactcca ggaaagaaga tttggcttga aggtgtggtg accacctcag ctggaggcac 1861 aaacaatcta tccgattcct atgctgcagg attcttatgg ttgaacactt taggaatgct 1921 ggccaatcag ggcattgatg tcgtgatacg gcactcattt tttgaccatg gatacaatca 1981 cctcgtggac cagaatttta acccattacc agactactgg ctctctctcc tctacaagcg 2041 cctgatcggc cccaaagtct tggctgtgca tgtggctggg ctccagcgga agccacggcc 2101 tggccgagtg atccgggaca aactaaggat ttatgctcac tgcacaaacc accacaacca 2161 caactacgtt cgtgggtcca ttacactttt tatcatcaac ttgcatcgat caagaaagaa 2221 aatcaagctg actgggactc tcagagacaa gctggttcac cagtacctgc tgcagcccta 2281 tgggcaggag ggcctaaagt ccaagtcagt gcaactgaat ggccagccct tagtgatggt 2341 ggacgacggg accctcccag aattgaagcc ccgccccctt cgggccggcc ggacattggt 2401 catccctcca gtcaccatgg gcttttttgt ggtcaagaat gtcaatgctt tggcctgccg 2461 ctaccgataa CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 2521 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA 2581 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT 2641 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA 2701 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC 2761 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG 2821 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC 2881 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT 2941 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC 3001 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT 3061 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG 3121 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC 3181 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG 3241 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC 3301 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 3361 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC 3421 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA 3481 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT 3541 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG 3601 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC 3661 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3721 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3781 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3841 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3901 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3961 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 4021 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 4081 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 4141 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 4201 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 4261 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4321 TTGCTGGCCT TTTGCTCACA TGTT //