LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2003..2099) /label=attL2 /note="attL2 (c)" misc_signal complement(2114..2133) /note="T7 Promoter/priming site (c)" primer_bind 2141..2157 /note="M13 Reverse priming site" gene 2270..3079 /note="Kanamycin resistance gene" rep_origin 3200..3873 /note="pUC origin" vector join(2011..3876,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2002 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:168630 IMAGE:9021007" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_310" /note="Vector: pCR4-TOPO; Clone identification sequence tag: GATCGACA" gene 692..2002 /gene="MANEA" /gene_synonym=hEndo /db_xref="GeneID:79694" /db_xref="HGNC:21072" misc_feature 692..2002 /gene="MANEA" /gene_synonym=hEndo /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..2002 /dnas_title="mannosidase, endo-alpha" /gene="MANEA" /gene_synonym=hEndo /codon_start=1 /product="mannosidase, endo-alpha" /protein_id="AAI37015.1" /db_xref="GI:187951585" /db_xref="GeneID:79694" /db_xref="HGNC:21072" /translation="MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDL LPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNY LHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPDDIGSSFYPELGS YSSRDPSVIETHMRQMRSASIGVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLK VTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPE KWANLLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSS HQNWASLKLFCDKYNLIFIPSVGPGYIDTSIRPWNTQNTRNRINGKYYEIGLSAALQT RPSLISITSFNEWHEGTQIEKAVPKRTSNTVYLDYRPHKPGLYLELTRKWSEKYSKER ATYALDRQLPVS" misc_feature 692..2002 /note="MANEA coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaaatgctg agaccaaata cagctacttt 721 tggagctcct tttggacttg accttcttcc agaacttcat caacgaacta ttcatttggg 781 gaaaaatttt gatttccaaa agagtgacag aatcaacagt gaaacaaata ccaagaattt 841 aaaaagtgtt gaaatcacta tgaaaccttc caaagcctct gaacttaact tggatgaact 901 accacctctg aacaattatc tacatgtatt ttattacagt tggtatggaa atccacaatt 961 tgatggtaaa tatatacatt ggaatcatcc agtgttagag cattgggacc ctagaatagc 1021 caagaattat ccacaaggga gacacaaccc tccagatgac attggctcca gcttttatcc 1081 tgaattggga agttacagtt ctcgggatcc ttctgtcata gaaactcaca tgagacaaat 1141 gcgctcagct tcaattggtg tactagccct ctcttggtac ccacctgatg taaatgatga 1201 aaatggagaa cctactgata acttggtacc cactattttg gataaagctc ataaatataa 1261 cctaaaggtt acttttcaca tagaaccata tagcaatcga gatgatcaaa acatgtacaa 1321 aaatgtcaag tatattatag acaaatatgg aaatcatccg gccttttaca ggtacaagac 1381 gaagactggc aatgctcttc ctatgtttta tgtctatgat tcctatatta ccaagcctga 1441 aaaatgggcc aatctgttaa ccacctcagg gtctcggagt attcgcaatt ctccttatga 1501 tggactgttt attgcccttc tggtagaaga aaaacataag tatgatattc ttcaaagtgg 1561 ttttgatgga atttacacat attttgccac aaatggcttt acttatggct catcacatca 1621 gaattgggct agcctaaaat tattttgtga taaatacaac ttaatattta tcccaagtgt 1681 gggcccagga tacatagata ccagcatccg tccatggaac acgcaaaaca ctcggaaccg 1741 aatcaatggg aagtattatg aaattggtct gagtgccgca cttcagacac gccccagctt 1801 aatttctatc acctctttta atgagtggca tgaaggaact cagattgaaa aagctgttcc 1861 caaaagaacc agtaatacag tgtacctaga ttaccgtcct cataaaccag gtctttacct 1921 agaactgact cgcaagtggt ctgaaaaata cagtaaggaa agagcaactt atgcattaga 1981 tcgccagctg cctgtttctt aaCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2041 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2101 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2161 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2221 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2281 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2341 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2401 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2461 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2521 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2581 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2641 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2701 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2761 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 2821 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 2881 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 2941 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3001 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3061 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3121 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3181 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3241 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3301 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3361 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3421 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3481 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3541 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3601 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3661 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3721 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3781 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 3841 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //