LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1190..1286) /label=attL2 /note="attL2 (c)" misc_signal complement(1301..1320) /note="T7 Promoter/priming site (c)" primer_bind 1328..1344 /note="M13 Reverse priming site" gene 1457..2266 /note="Kanamycin resistance gene" rep_origin 2387..3060 /note="pUC origin" PCR_frame 669..1190 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1210) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1207) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1198..3063,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1168..1188 /note="TEV site" source 673..1167 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:12423 IMAGE:3996922" /tissue_type="Bladder, carcinoma" /clone_lib="NIH_MGC_53" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 673..1167 /gene="ALG13" /gene_synonym=MDS031 /gene_synonym=YGL047W /db_xref="GeneID:79868" /db_xref="HGNC:30881" CDS 673..1167 /dnas_title="asparagine-linked glycosylation 13 homolog (S. cerevisiae)" /gene="ALG13" /gene_synonym=MDS031 /gene_synonym=YGL047W /codon_start=1 /product="asparagine-linked glycosylation 13 homolog (S. cerevisiae)" /protein_id="AAH05336.1" /db_xref="GI:13529128" /db_xref="GeneID:79868" /db_xref="HGNC:30881" /translation="MKCVFVTVGTTSFDDLIACVSAPDSLQKIESLGYNRLILQIGRG TVVPEPFSTESFTLDVYRYKDSLKEDIQKADLVISHAGAGSCLETLEKGKPLVVVINE KLMNNHQLELAKQLHKEGHLFYCTCSTLPGLLQSMDLSTLKCYPPGQPEKFSAFLDKV VGLQK" misc_feature 673..1167 /note="ALG13 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaagtg cgtgtttgtt accgtaggga ccaccagctt tgacgacctc 721 attgcgtgtg tgtcggcgcc cgacagtctg caaaaaatcg agagccttgg ttacaaccga 781 cttatcctgc aaattggtag aggaacggtg gtacctgaac ccttcagtac tgagtcgttt 841 actctggatg tttacaggta caaggattcc ttgaaagaag acattcagaa agcagatctt 901 gttattagtc acgcaggtgc aggaagctgt ttggagactc tggaaaaagg aaagccactc 961 gtagtggtta taaacgaaaa gttgatgaac aatcatcagc tggaactggc aaagcagcta 1021 cacaaagagg gtcatctctt ctattgtacc tgcagcacgc ttcctgggct gttacagtca 1081 atggacttat caacactgaa atgttatcct cctggccagc cagaaaaatt ttctgcattt 1141 ttggataaag ttgttggatt acaaaaagaa aacttgtact ttcaaggctC CAGCTTTCTT 1201 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 1261 TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA 1321 TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA 1381 CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 1441 TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC 1501 CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG 1561 TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG 1621 CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA 1681 ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT 1741 CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG 1801 TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG 1861 TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA 1921 TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA 1981 AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG 2041 TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT 2101 TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG 2161 TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA 2221 TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA 2281 TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC 2341 AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 2401 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 2461 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 2521 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT 2581 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 2641 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 2701 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 2761 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 2821 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 2881 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 2941 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3001 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3061 GTT //