LOCUS UGT1A1-pGEc1-DEST 5965 bp DNA circular 04-DEC-2012 FEATURES Location/Qualifiers misc_feature complement(5443..5572) /note="3'-ITR" misc_feature complement(5141..5355) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey=33 CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey=4 misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4593..5134 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" misc_recomb 4498..4520 /label=attB2 /note="attB2 site" misc_feature 4530..4553 /note="8xHis Tag" misc_feature 4557..4580 /note="StrepTag" PCR_frame 3002..4498 /note="Reading Frame" /set="Set 1" PCR_primer 2985..3001 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC source 3006..4475 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:156187 IMAGE:40117598" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: TATACTAT sequenced from the reverse primer" gene 3006..4475 /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" CDS 3006..4475 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A1" /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A1" /protein_id="AAI28415.1" /db_xref="GI:118763983" /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" /translation="MAVESQGGRPLVLGLLLCVLGPVVSHAGKILLIPVDGSHWLSML GAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFEN DSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIV AQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSHSDHMTFLQRVKNMLIAFS QNFLCDVVYSPYATLASEFLQREVTVQDLLSSASVWLFRSDFVKDYPRPIMPNMVFVG GINCLHQNPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVL WRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMP LFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDR PVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFK CCAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 3006..4475 /note="UGT1A1 coding region" misc_feature 4476..4496 /note="TEV site" PCR_primer 2985..3001 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(3002..4505) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(2985..4505) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(4506..5965,1..2984) /source="pGEc1-DEST" /type="Destination Vector" clone 2985..4505 /source="UGT1A1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc tgtggagtcc cagggcggac gcccacttgt cctgggcctg ctgctgtgtg 3061 tgctgggccc agtggtgtcc catgctggga agatactgtt gatcccagtg gatggcagcc 3121 actggctgag catgcttggg gccatccagc agctgcagca gaggggacat gaaatagttg 3181 tcctagcacc tgacgcctcg ttgtacatca gagacggagc attttacacc ttgaagacgt 3241 accctgtgcc attccaaagg gaggatgtga aagagtcttt tgttagtctc gggcataatg 3301 tttttgagaa tgattctttc ctgcagcgtg tgatcaaaac atacaagaaa ataaaaaagg 3361 actctgctat gcttttgtct ggctgttccc acttactgca caacaaggag ctcatggcct 3421 ccctggcaga aagcagcttt gatgtcatgc tgacggaccc tttccttcct tgcagcccca 3481 tcgtggccca gtacctgtct ctgcccactg tattcttctt gcatgcactg ccatgcagcc 3541 tggaatttga ggctacccag tgccccaacc cattctccta cgtgcccagg cctctctcct 3601 ctcattcaga tcacatgacc ttcctgcagc gggtgaagaa catgctcatt gccttttcac 3661 agaactttct gtgcgacgtg gtttattccc cgtatgcaac ccttgcctca gaattccttc 3721 agagagaggt gactgtccag gacctattga gctctgcatc tgtctggctg tttagaagtg 3781 actttgtgaa ggattaccct aggcccatca tgcccaatat ggtttttgtt ggtggaatca 3841 actgccttca ccaaaatcca ctatcccagg aatttgaagc ctacattaat gcttctggag 3901 aacatggaat tgtggttttc tctttgggat caatggtctc agaaattcca gagaagaaag 3961 ctatggcaat tgctgatgct ttgggcaaaa tccctcagac agtcctgtgg cggtacactg 4021 gaacccgacc atcgaatctt gcgaacaaca cgatacttgt taagtggcta ccccaaaacg 4081 atctgcttgg tcacccgatg acccgtgcct ttatcaccca tgctggttcc catggtgttt 4141 atgaaagcat atgcaatggc gttcccatgg tgatgatgcc cttgtttggt gatcagatgg 4201 acaatgcaaa gcgcatggag actaagggag ctggagtgac cctgaatgtt ctggaaatga 4261 cttctgaaga tttagaaaat gctctaaaag cagtcatcaa tgacaaaagt tacaaggaga 4321 acatcatgcg cctctccagc cttcacaagg accgcccggt ggagccgctg gacctggccg 4381 tgttctgggt ggagtttgtg atgaggcaca agggcgcgcc acacctgcgc cccgcagccc 4441 acgacctcac ctggtaccag taccattcct tggacgaaaa cttgtacttt caaggctCCA 4501 GCTTTCTTGT ACAAAGTGGT GATGGCCGCC ATCATCATCA TCATCATCAT CATGCGTGGA 4561 GCCACCCGCA GTTCGAAAAA TAATAAGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG 4621 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 4681 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 4741 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 4801 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 4861 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 4921 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 4981 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 5041 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 5101 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG 5161 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 5221 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 5281 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 5341 CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG 5401 GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG 5461 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG 5521 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT 5581 AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC 5641 GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 5701 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 5761 AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC 5821 CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 5881 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 5941 CAAAATATTA ACGCTTACAA TTTAG //