LOCUS UGT1A1-pGEc2-DEST 6691 bp DNA circular 04-DEC-2012 FEATURES Location/Qualifiers misc_feature complement(6169..6298) /note="3'-ITR" misc_feature complement(5867..6081) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey=33 CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey=4 misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5319..5860 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" misc_feature 4498..4520 /note="attB2" misc_feature 5298..5303 /note="BstB1 site" misc_feature 4521..5216 /note="Super GFP" misc_feature 5217..5267 /note="AviTag" misc_feature 5268..5291 /note="His Tag" PCR_frame 3002..4498 /note="Reading Frame" /set="Set 1" PCR_primer 2985..3001 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC source 3006..4475 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:156187 IMAGE:40117598" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: TATACTAT sequenced from the reverse primer" gene 3006..4475 /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" CDS 3006..4475 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A1" /gene="UGT1A1" /gene_synonym=HUG-BR1 /gene_synonym=UDPGT /gene_synonym=UGT1*1 /gene_synonym=UGT1A /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A1" /protein_id="AAI28415.1" /db_xref="GI:118763983" /db_xref="GeneID:54658" /db_xref="HGNC:12530" /db_xref="MIM:191740" /translation="MAVESQGGRPLVLGLLLCVLGPVVSHAGKILLIPVDGSHWLSML GAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFEN DSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIV AQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSHSDHMTFLQRVKNMLIAFS QNFLCDVVYSPYATLASEFLQREVTVQDLLSSASVWLFRSDFVKDYPRPIMPNMVFVG GINCLHQNPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVL WRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMP LFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDR PVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFK CCAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 3006..4475 /note="UGT1A1 coding region" misc_feature 4476..4496 /note="TEV site" PCR_primer 2985..3001 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(3002..4505) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(2985..4505) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(4506..6691,1..2984) /source="pGEc2-DEST" /type="Destination Vector" clone 2985..4505 /source="UGT1A1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc tgtggagtcc cagggcggac gcccacttgt cctgggcctg ctgctgtgtg 3061 tgctgggccc agtggtgtcc catgctggga agatactgtt gatcccagtg gatggcagcc 3121 actggctgag catgcttggg gccatccagc agctgcagca gaggggacat gaaatagttg 3181 tcctagcacc tgacgcctcg ttgtacatca gagacggagc attttacacc ttgaagacgt 3241 accctgtgcc attccaaagg gaggatgtga aagagtcttt tgttagtctc gggcataatg 3301 tttttgagaa tgattctttc ctgcagcgtg tgatcaaaac atacaagaaa ataaaaaagg 3361 actctgctat gcttttgtct ggctgttccc acttactgca caacaaggag ctcatggcct 3421 ccctggcaga aagcagcttt gatgtcatgc tgacggaccc tttccttcct tgcagcccca 3481 tcgtggccca gtacctgtct ctgcccactg tattcttctt gcatgcactg ccatgcagcc 3541 tggaatttga ggctacccag tgccccaacc cattctccta cgtgcccagg cctctctcct 3601 ctcattcaga tcacatgacc ttcctgcagc gggtgaagaa catgctcatt gccttttcac 3661 agaactttct gtgcgacgtg gtttattccc cgtatgcaac ccttgcctca gaattccttc 3721 agagagaggt gactgtccag gacctattga gctctgcatc tgtctggctg tttagaagtg 3781 actttgtgaa ggattaccct aggcccatca tgcccaatat ggtttttgtt ggtggaatca 3841 actgccttca ccaaaatcca ctatcccagg aatttgaagc ctacattaat gcttctggag 3901 aacatggaat tgtggttttc tctttgggat caatggtctc agaaattcca gagaagaaag 3961 ctatggcaat tgctgatgct ttgggcaaaa tccctcagac agtcctgtgg cggtacactg 4021 gaacccgacc atcgaatctt gcgaacaaca cgatacttgt taagtggcta ccccaaaacg 4081 atctgcttgg tcacccgatg acccgtgcct ttatcaccca tgctggttcc catggtgttt 4141 atgaaagcat atgcaatggc gttcccatgg tgatgatgcc cttgtttggt gatcagatgg 4201 acaatgcaaa gcgcatggag actaagggag ctggagtgac cctgaatgtt ctggaaatga 4261 cttctgaaga tttagaaaat gctctaaaag cagtcatcaa tgacaaaagt tacaaggaga 4321 acatcatgcg cctctccagc cttcacaagg accgcccggt ggagccgctg gacctggccg 4381 tgttctgggt ggagtttgtg atgaggcaca agggcgcgcc acacctgcgc cccgcagccc 4441 acgacctcac ctggtaccag taccattcct tggacgaaaa cttgtacttt caaggctCCA 4501 GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA 4561 TCCTGGTGGA ACTGGATGGC GACGTGAACG GCCACAAGTT CTCTGTGCGG GGAGAGGGCG 4621 AAGGCGACGC CACAAATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 4681 CCGTGCCTTG GCCTACCCTC GTGACCACAC TGACCTACGG CGTGCAGTGC TTCAGCAGAT 4741 ACCCCGACCA TATGAAGCGG CACGACTTCT TCAAGAGCGC CATGCCCGAG GGCTACGTGC 4801 AGGAACGGAC CATCAGCTTC AAGGACGACG GCACCTACAA GACCAGAGCC GAAGTGAAGT 4861 TCGAGGGCGA CACCCTCGTG AACCGGATCG AGCTGAAGGG CATCGATTTC AAAGAGGACG 4921 GCAACATCCT GGGCCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTG TACATCACCG 4981 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC GTGGAAGATG 5041 GCAGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGAGAT GGCCCCGTGC 5101 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCGTGCT GAGCAAGGAC CCCAACGAGA 5161 AGCGGGACCA CATGGTGCTG CTGGAATTCG TGACCGCCGC TGGCATCACC CACGGCATGA 5221 GCGGCCTGAA CGACATCTTC GAGGCCCAGA AAATCGAGTG GCACGAGCAC CACCATCATC 5281 ACCATCACCA CTGATGATTC GAAAAATAAT AAGGATCCAA TCAACCTCTG GATTACAAAA 5341 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 5401 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 5461 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 5521 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 5581 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 5641 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 5701 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 5761 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 5821 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 5881 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 5941 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 6001 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 6061 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 6121 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 6181 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 6241 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 6301 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 6361 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 6421 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 6481 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 6541 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 6601 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 6661 TTTTAACAAA ATATTAACGC TTACAATTTA G //