LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2156..2252) /label=attL2 /note="attL2 (c)" misc_signal complement(2267..2286) /note="T7 Promoter/priming site (c)" primer_bind 2294..2310 /note="M13 Reverse priming site" gene 2423..3232 /note="Kanamycin resistance gene" rep_origin 3353..4026 /note="pUC origin" PCR_frame 669..2156 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2176) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2173) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2164..4029,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2134..2154 /note="TEV site" source 673..2133 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9353 IMAGE:3850654" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_65" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2133 /gene="UGT1A10" /gene_synonym=UDPGT /gene_synonym=UGT1J /db_xref="GeneID:54575" /db_xref="HGNC:12531" /db_xref="MIM:606435" CDS 673..2133 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A10" /gene="UGT1A10" /gene_synonym=UDPGT /gene_synonym=UGT1J /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A10" /protein_id="AAH20971.1" /db_xref="GI:18088087" /db_xref="GeneID:54575" /db_xref="HGNC:12531" /db_xref="MIM:606435" /translation="MARAGWTSPVPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMQSV VEKLILRGHEVVVVMPEVSWQLERSLNCTVKTYSTSYTLEDQNREFMVFAHAQWKAQA QSIFSLLMSSSSGFLDLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDTCGLIVAKY FSLPSVVFTRGIFCHHLEEGAQCPAPLSYVPNDLLGFSDAMTFKERVWNHIVHLEDHL FCQYLFRNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGIN CHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRY TGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFG DQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVE PLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCA YGYRKCLGKKGRVKKAHKSKTH" misc_feature 673..2133 /note="UGT1A10 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctcg cgcagggtgg accagccccg ttcctttatg tgtgtgtcta 721 ctgctgacct gtggctttgc cgaggcaggg aagctgctgg tagtgcccat ggatgggagt 781 cactggttca ccatgcagtc ggtggtggag aaacttatcc tcagggggca tgaggtggtt 841 gtagtcatgc cagaggtgag ttggcaactg gaaagatcac tgaattgcac agtgaagact 901 tactcaacct cgtacactct ggaagatcag aaccgggaat tcatggtttt cgcccatgct 961 caatggaaag cacaggcaca aagtatattt tctctattaa tgagttcatc cagtggtttt 1021 cttgacttat ttttttcgca ttgcaggagt ttgtttaatg accgaaaatt agtagaatac 1081 ttaaaggaga gttcttttga tgcagtgttt ctggatcctt ttgatacctg tggcttaatt 1141 gttgctaaat atttctccct cccctctgtg gtcttcacca ggggaatatt ttgccaccat 1201 cttgaagaag gtgcacagtg ccctgctcct ctttcctatg tccccaatga tctcttaggg 1261 ttctcagatg ccatgacttt caaggagaga gtatggaacc acatcgtgca cttggaggac 1321 catttatttt gccagtatct ttttagaaat gccctagaaa tagcctctga aattctccaa 1381 acccctgtca cggcatatga tctctacagt cacacatcaa tttggttgtt gcgaacggac 1441 tttgttttgg actatcccaa acccgtgatg cccaacatga tcttcattgg tggtatcaac 1501 tgtcatcagg gaaagccatt gcctatggaa tttgaagcct acattaatgc ttctggagaa 1561 catggaattg tggttttctc tttgggatca atggtctcag aaattccaga gaagaaagct 1621 atggcaattg ctgatgcttt gggcaaaatc cctcagacag tcctgtggcg gtacactgga 1681 acccgaccat cgaatcttgc gaacaacacg atacttgtta agtggctacc ccaaaacgat 1741 ctgcttggtc acccgatgac ccgtgccttt atcacccatg ctggttccca tggtgtttat 1801 gaaagcatat gcaatggcgt tcccatggtg atgatgccct tgtttggtga tcagatggac 1861 aatgcaaagc gcatggagac taagggagct ggagtgaccc tgaatgttct ggaaatgact 1921 tctgaagatt tagaaaatgc tctaaaagca gtcatcaatg acaaaagtta caaggagaac 1981 atcatgcgcc tctccagcct tcacaaggac cgcccggtgg agccgctgga cctggccgtg 2041 ttctgggtgg agtttgtgat gaggcacaag ggcgcgccac acctgcgccc cgcagcccac 2101 gacctcacct ggtaccagta ccattccttg gacgaaaact tgtactttca aggctCCAGC 2161 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2221 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2281 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2341 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2401 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2461 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2521 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2581 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2641 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2701 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2761 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2821 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2881 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2941 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3001 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3061 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3121 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3181 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3241 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3301 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3361 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3421 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3481 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3541 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3601 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3661 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3721 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3781 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3841 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3901 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3961 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4021 TCACATGTT //