LOCUS UGT1A6-pGEc2-DES 6688 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6166..6295) /note="3'-ITR" misc_feature complement(5864..6078) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5316..5857 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4495..4517 /note="attB2" misc_feature 5295..5300 /note="BstB1 site" misc_feature 4518..5213 /note="Super GFP" misc_feature 5214..5264 /note="AviTag" misc_feature 5265..5288 /note="His Tag" PCR_frame 3002..4495 /note="Reading Frame" /set="Set 1" source 3006..4472 /dnas_title="UGT1A6-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29860 IMAGE:4454322" /tissue_type="Kidney, hypernephroma" /clone_lib="NIH_MGC_89" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4472 /dnas_title="UGT1A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" CDS 3006..4472 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A6" /protein_id="AAH19861.1" /db_xref="GI:18043701" /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" /translation="MACLLRSFQRISAGVFFLALWGMVVGDKLLVVPQDGSHWLSMKD IVEVLSDRGHEIVVVVPEVNLLLKESKYYTRKIYPVPYDQEELKNRYQSFGNNHFAER SFLTAPQTEYRNNMIVIGLYFINCQSLLQDRDTLNFFKESKFDALFTDPALPCGVILA EYLGLPSVYLFRGFPCSLEHTFSRSPDPVSYIPRCYTKFSDHMTFSQRVANFLVNLLE PYLFYCLFSKYEELASAVLKRDVDIITLYQKVSVWLLRYDFVLEYPRPVMPNMVFIGG INCKKRKDLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMATADALGKIPQTVLW RYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPL FGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRP VEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKC CAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 3006..4472 /note="UGT1A6 coding region" misc_difference 3967..3967 /dnas_title="UGT1A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'T' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4473..4493 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4502) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4502) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4503..6688,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4502 /source="UGT1A6-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ctgcctcctt cgctcatttc agagaatttc tgcaggggtt ttcttcttag 3061 cactttgggg catggttgta ggtgacaagc tgctggtggt ccctcaggac ggaagccact 3121 ggcttagtat gaaggatata gttgaggttc tcagtgaccg gggtcatgag attgtagtgg 3181 tggtgcctga agttaatttg cttttgaaag aatccaaata ctacacaaga aaaatctatc 3241 cagtgccgta tgaccaagaa gagctgaaga accgttacca atcatttgga aacaatcact 3301 ttgctgagcg atcattccta actgctcctc agacagagta caggaataac atgattgtta 3361 ttggcctgta cttcatcaac tgccagagcc tcctgcagga cagggacacc ctgaacttct 3421 ttaaggagag caagtttgat gctcttttca cagacccagc cttaccctgt ggggtgatcc 3481 tggctgagta tttgggccta ccatctgtgt acctcttcag gggttttccg tgttccctgg 3541 agcatacatt cagcagaagc ccagaccctg tgtcctacat tcccaggtgc tacacaaagt 3601 tttcagacca catgactttt tcccaacgag tggccaactt ccttgttaat ttgttggagc 3661 cctatctatt ttattgtctg ttttcaaagt atgaagaact cgcatcagct gtcctcaaga 3721 gagatgtgga tataatcacc ttatatcaga aggtctctgt ttggctgtta agatatgact 3781 ttgtgcttga atatcctagg ccggtcatgc ccaacatggt cttcattgga ggtatcaact 3841 gtaagaagag gaaagacttg tctcaggaat ttgaagccta cattaatgct tctggagaac 3901 atggaattgt ggttttctct ttgggatcaa tggtctcaga aattccagag aagaaagcta 3961 tggcaactgc tgatgctttg ggcaaaatcc ctcagacagt cctgtggcgg tacactggaa 4021 cccgaccatc gaatcttgcg aacaacacga tacttgttaa gtggctaccc caaaacgatc 4081 tgcttggtca cccgatgacc cgtgccttta tcacccatgc tggttcccat ggtgtttatg 4141 aaagcatatg caatggcgtt cccatggtga tgatgccctt gtttggtgat cagatggaca 4201 atgcaaagcg catggagact aagggagctg gagtgaccct gaatgttctg gaaatgactt 4261 ctgaagattt agaaaatgct ctaaaagcag tcatcaatga caaaagttac aaggagaaca 4321 tcatgcgcct ctccagcctt cacaaggacc gcccggtgga gccgctggac ctggccgtgt 4381 tctgggtgga gtttgtgatg aggcacaagg gcgcgccaca cctgcgcccc gcagcccacg 4441 acctcacctg gtaccagtac cattccttgg acgaaaactt gtactttcaa ggctCCAGCT 4501 TTCTTGTACA AAGTGGTATG AGCAAGGGCG AGGAACTGTT CACCGGCGTG GTGCCCATCC 4561 TGGTGGAACT GGATGGCGAC GTGAACGGCC ACAAGTTCTC TGTGCGGGGA GAGGGCGAAG 4621 GCGACGCCAC AAATGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG 4681 TGCCTTGGCC TACCCTCGTG ACCACACTGA CCTACGGCGT GCAGTGCTTC AGCAGATACC 4741 CCGACCATAT GAAGCGGCAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG 4801 AACGGACCAT CAGCTTCAAG GACGACGGCA CCTACAAGAC CAGAGCCGAA GTGAAGTTCG 4861 AGGGCGACAC CCTCGTGAAC CGGATCGAGC TGAAGGGCAT CGATTTCAAA GAGGACGGCA 4921 ACATCCTGGG CCACAAGCTG GAGTACAACT TCAACAGCCA CAACGTGTAC ATCACCGCCG 4981 ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACGTG GAAGATGGCA 5041 GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT CGGAGATGGC CCCGTGCTGC 5101 TGCCCGACAA CCACTACCTG AGCACCCAGA GCGTGCTGAG CAAGGACCCC AACGAGAAGC 5161 GGGACCACAT GGTGCTGCTG GAATTCGTGA CCGCCGCTGG CATCACCCAC GGCATGAGCG 5221 GCCTGAACGA CATCTTCGAG GCCCAGAAAA TCGAGTGGCA CGAGCACCAC CATCATCACC 5281 ATCACCACTG ATGATTCGAA AAATAATAAG GATCCAATCA ACCTCTGGAT TACAAAATTT 5341 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5401 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 5461 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 5521 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 5581 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 5641 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 5701 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 5761 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 5821 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC 5881 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 5941 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 6001 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 6061 TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC GATTAGGATC TTCCTAGAGC 6121 ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA 6181 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG 6241 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA 6301 ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC GCCAATTCGC CCTATAGTGA 6361 GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 6421 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 6481 AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAG 6541 CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 6601 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 6661 TAACAAAATA TTAACGCTTA CAATTTAG //