LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2162..2258) /label=attL2 /note="attL2 (c)" misc_signal complement(2273..2292) /note="T7 Promoter/priming site (c)" primer_bind 2300..2316 /note="M13 Reverse priming site" gene 2429..3238 /note="Kanamycin resistance gene" rep_origin 3359..4032 /note="pUC origin" PCR_frame 669..2162 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2182) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2179) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2170..4035,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2140..2160 /note="TEV site" source 673..2139 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29860 IMAGE:4454322" /tissue_type="Kidney, hypernephroma" /clone_lib="NIH_MGC_89" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2139 /gene="UGT1A6" /gene_synonym=GNT1 /gene_synonym=HLUGP /gene_synonym=HLUGP1 /gene_synonym=MGC29860 /gene_synonym=UDPGT /gene_synonym=UGT1 /gene_synonym=UGT1*6 /gene_synonym=UGT1F /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" CDS 673..2139 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A6" /gene="UGT1A6" /gene_synonym=GNT1 /gene_synonym=HLUGP /gene_synonym=HLUGP1 /gene_synonym=MGC29860 /gene_synonym=UDPGT /gene_synonym=UGT1 /gene_synonym=UGT1*6 /gene_synonym=UGT1F /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A6" /protein_id="AAH19861.1" /db_xref="GI:18043701" /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" /translation="MACLLRSFQRISAGVFFLALWGMVVGDKLLVVPQDGSHWLSMKD IVEVLSDRGHEIVVVVPEVNLLLKESKYYTRKIYPVPYDQEELKNRYQSFGNNHFAER SFLTAPQTEYRNNMIVIGLYFINCQSLLQDRDTLNFFKESKFDALFTDPALPCGVILA EYLGLPSVYLFRGFPCSLEHTFSRSPDPVSYIPRCYTKFSDHMTFSQRVANFLVNLLE PYLFYCLFSKYEELASAVLKRDVDIITLYQKVSVWLLRYDFVLEYPRPVMPNMVFIGG INCKKRKDLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMATADALGKIPQTVLW RYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPL FGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRP VEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKC CAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 673..2139 /note="UGT1A6 coding region" misc_difference 1634..1634 /gene="UGT1A6" /gene_synonym=GNT1 /gene_synonym=HLUGP /gene_synonym=HLUGP1 /gene_synonym=MGC29860 /gene_synonym=UDPGT /gene_synonym=UGT1 /gene_synonym=UGT1*6 /gene_synonym=UGT1F /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'T' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcctg cctccttcgc tcatttcaga gaatttctgc aggggttttc 721 ttcttagcac tttggggcat ggttgtaggt gacaagctgc tggtggtccc tcaggacgga 781 agccactggc ttagtatgaa ggatatagtt gaggttctca gtgaccgggg tcatgagatt 841 gtagtggtgg tgcctgaagt taatttgctt ttgaaagaat ccaaatacta cacaagaaaa 901 atctatccag tgccgtatga ccaagaagag ctgaagaacc gttaccaatc atttggaaac 961 aatcactttg ctgagcgatc attcctaact gctcctcaga cagagtacag gaataacatg 1021 attgttattg gcctgtactt catcaactgc cagagcctcc tgcaggacag ggacaccctg 1081 aacttcttta aggagagcaa gtttgatgct cttttcacag acccagcctt accctgtggg 1141 gtgatcctgg ctgagtattt gggcctacca tctgtgtacc tcttcagggg ttttccgtgt 1201 tccctggagc atacattcag cagaagccca gaccctgtgt cctacattcc caggtgctac 1261 acaaagtttt cagaccacat gactttttcc caacgagtgg ccaacttcct tgttaatttg 1321 ttggagccct atctatttta ttgtctgttt tcaaagtatg aagaactcgc atcagctgtc 1381 ctcaagagag atgtggatat aatcacctta tatcagaagg tctctgtttg gctgttaaga 1441 tatgactttg tgcttgaata tcctaggccg gtcatgccca acatggtctt cattggaggt 1501 atcaactgta agaagaggaa agacttgtct caggaatttg aagcctacat taatgcttct 1561 ggagaacatg gaattgtggt tttctctttg ggatcaatgg tctcagaaat tccagagaag 1621 aaagctatgg caactgctga tgctttgggc aaaatccctc agacagtcct gtggcggtac 1681 actggaaccc gaccatcgaa tcttgcgaac aacacgatac ttgttaagtg gctaccccaa 1741 aacgatctgc ttggtcaccc gatgacccgt gcctttatca cccatgctgg ttcccatggt 1801 gtttatgaaa gcatatgcaa tggcgttccc atggtgatga tgcccttgtt tggtgatcag 1861 atggacaatg caaagcgcat ggagactaag ggagctggag tgaccctgaa tgttctggaa 1921 atgacttctg aagatttaga aaatgctcta aaagcagtca tcaatgacaa aagttacaag 1981 gagaacatca tgcgcctctc cagccttcac aaggaccgcc cggtggagcc gctggacctg 2041 gccgtgttct gggtggagtt tgtgatgagg cacaagggcg cgccacacct gcgccccgca 2101 gcccacgacc tcacctggta ccagtaccat tccttggacg aaaacttgta ctttcaaggc 2161 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2221 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2281 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2341 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2401 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2461 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2521 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2581 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2641 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2701 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2761 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2821 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2881 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2941 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3001 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3061 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3121 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3181 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3241 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3301 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3361 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3421 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3481 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3541 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3601 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3661 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3721 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3781 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3841 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3901 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3961 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4021 TTTTGCTCAC ATGTT //