LOCUS UGT1A6-pGEn1-DES 5971 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4599..5140 /note="WPRE" misc_recomb 4561..4583 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5147..5361) /note="BGH pA" misc_feature complement(5449..5578) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4554 /dnas_title="UGT1A6-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29860 IMAGE:4454322" /tissue_type="Kidney, hypernephroma" /clone_lib="NIH_MGC_89" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3166..4554 /dnas_title="UGT1A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" CDS 3166..4554 /dnas_title="UDP glucuronosyltransferase 1 family, polypeptide A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /codon_start=1 /product="UDP glucuronosyltransferase 1 family, polypeptide A6" /protein_id="AAH19861.1" /db_xref="GI:18043701" /db_xref="GeneID:54578" /db_xref="HGNC:12538" /db_xref="MIM:606431" /translation="MACLLRSFQRISAGVFFLALWGMVVGDKLLVVPQDGSHWLSMKD IVEVLSDRGHEIVVVVPEVNLLLKESKYYTRKIYPVPYDQEELKNRYQSFGNNHFAER SFLTAPQTEYRNNMIVIGLYFINCQSLLQDRDTLNFFKESKFDALFTDPALPCGVILA EYLGLPSVYLFRGFPCSLEHTFSRSPDPVSYIPRCYTKFSDHMTFSQRVANFLVNLLE PYLFYCLFSKYEELASAVLKRDVDIITLYQKVSVWLLRYDFVLEYPRPVMPNMVFIGG INCKKRKDLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMATADALGKIPQTVLW RYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPL FGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRP VEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKC CAYGYRKCLGKKGRVKKAHKSKTH" misc_feature 3166..4554 /note="UGT1A6 coding region" misc_difference 4049..4049 /dnas_title="UGT1A6" /gene="UGT1A6" /gene_synonym="GNT1" /gene_synonym="HLUGP" /gene_synonym="HLUGP1" /gene_synonym="MGC29860" /gene_synonym="UDPGT" /gene_synonym="UGT1" /gene_synonym="UGT1*6" /gene_synonym="UGT1F" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'T' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4569..5971,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4568 /source="UGT1A6_Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgacaa gctgctggtg 3181 gtccctcagg acggaagcca ctggcttagt atgaaggata tagttgaggt tctcagtgac 3241 cggggtcatg agattgtagt ggtggtgcct gaagttaatt tgcttttgaa agaatccaaa 3301 tactacacaa gaaaaatcta tccagtgccg tatgaccaag aagagctgaa gaaccgttac 3361 caatcatttg gaaacaatca ctttgctgag cgatcattcc taactgctcc tcagacagag 3421 tacaggaata acatgattgt tattggcctg tacttcatca actgccagag cctcctgcag 3481 gacagggaca ccctgaactt ctttaaggag agcaagtttg atgctctttt cacagaccca 3541 gccttaccct gtggggtgat cctggctgag tatttgggcc taccatctgt gtacctcttc 3601 aggggttttc cgtgttccct ggagcataca ttcagcagaa gcccagaccc tgtgtcctac 3661 attcccaggt gctacacaaa gttttcagac cacatgactt tttcccaacg agtggccaac 3721 ttccttgtta atttgttgga gccctatcta ttttattgtc tgttttcaaa gtatgaagaa 3781 ctcgcatcag ctgtcctcaa gagagatgtg gatataatca ccttatatca gaaggtctct 3841 gtttggctgt taagatatga ctttgtgctt gaatatccta ggccggtcat gcccaacatg 3901 gtcttcattg gaggtatcaa ctgtaagaag aggaaagact tgtctcagga atttgaagcc 3961 tacattaatg cttctggaga acatggaatt gtggttttct ctttgggatc aatggtctca 4021 gaaattccag agaagaaagc tatggcaact gctgatgctt tgggcaaaat ccctcagaca 4081 gtcctgtggc ggtacactgg aacccgacca tcgaatcttg cgaacaacac gatacttgtt 4141 aagtggctac cccaaaacga tctgcttggt cacccgatga cccgtgcctt tatcacccat 4201 gctggttccc atggtgttta tgaaagcata tgcaatggcg ttcccatggt gatgatgccc 4261 ttgtttggtg atcagatgga caatgcaaag cgcatggaga ctaagggagc tggagtgacc 4321 ctgaatgttc tggaaatgac ttctgaagat ttagaaaatg ctctaaaagc agtcatcaat 4381 gacaaaagtt acaaggagaa catcatgcgc ctctccagcc ttcacaagga ccgcccggtg 4441 gagccgctgg acctggccgt gttctgggtg gagtttgtga tgaggcacaa gggcgcgcca 4501 cacctgcgcc ccgcagccca cgacctcacc tggtaccagt accattcctt ggacTAGGAC 4561 CCAGCTTTCT TGTACAAAGT GGTTGATAGC TTGGATCCAA TCAACCTCTG GATTACAAAA 4621 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 4681 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 4741 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 4801 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 4861 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 4921 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 4981 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 5041 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 5101 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 5161 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 5221 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 5281 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 5341 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 5401 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 5461 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 5521 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 5581 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 5641 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 5701 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 5761 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 5821 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 5881 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 5941 TTTTAACAAA ATATTAACGC TTACAATTTA G //