LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2165..2261) /label=attL2 /note="attL2 (c)" misc_signal complement(2276..2295) /note="T7 Promoter/priming site (c)" primer_bind 2303..2319 /note="M13 Reverse priming site" gene 2432..3241 /note="Kanamycin resistance gene" rep_origin 3362..4035 /note="pUC origin" PCR_frame 669..2165 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2185) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2182) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2173..4038,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2143..2163 /note="TEV site" source 673..2142 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:163405 IMAGE:40146564" /tissue_type="Pooled, cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus, PCR rescued clones" /clone_lib="NIH_MGC_362" /lab_host="DH10B" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AAACGGTC" gene 673..2142 /gene="UGT2A3" /db_xref="GeneID:79799" /db_xref="HGNC:28528" CDS 673..2142 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide A3" /gene="UGT2A3" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide A3" /protein_id="AAI30534.1" /db_xref="GI:120660408" /db_xref="GeneID:79799" /db_xref="HGNC:28528" /translation="MRSDKSALVFLLLQLFCVGCGFCGKVLVWPCDMSHWLNVKVILE ELIVRGHEVTVLTHSKPSLIDYRKPSALKFEVVHMPQDRTEENEIFVDLALNVLPGLS TWQSVIKLNDFFVEIRGTLKMMCESFIYNQTLMKKLQETNYDVMLIDPVIPCGDLMAE LLAVPFVLTLRISVGGNMERSCGKLPAPLSYVPVPMTGLTDRMTFLERVKNSMLSVLF HFWIQDYDYHFWEEFYSKALGRPTTLCETVGKAEIWLIRTYWDFEFPQPYQPNFEFVG GLHCKPAKALPKEMENFVQSSGEDGIVVFSLGSLFQNVTEEKANIIASALAQIPQKVL WRYKGKKPSTLGANTRLYDWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVP IFGDQLDNIAHMKAKGAAVEINFKTMTSEDLLRALRTVITDSSYKENAMRLSRIHHDQ PVKPLDRAVFWIEFVMRHKGAKHLRSAAHDLTWFQHYSIDVIGFLLTCVATAIFLFTK CFLFSCQKFNKTRKIEKRE" misc_difference 1071..1071 /gene="UGT2A3" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2142 /note="UGT2A3 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaggtc tgacaagtca gctttggtat ttctgctcct gcagctcttc 721 tgtgttggct gtggattctg tgggaaagtc ctggtgtggc cctgtgacat gagccattgg 781 cttaatgtca aggtcattct agaagagctc atagtgagag gccatgaggt aacagtattg 841 actcactcaa agccttcgtt aattgactac aggaagcctt ctgcattgaa atttgaggtg 901 gtccatatgc cacaggacag aacagaagaa aatgaaatat ttgttgacct agctctgaat 961 gtcttgccag gcttatcaac ctggcaatca gttataaaat taaatgattt ttttgttgaa 1021 ataagaggaa ctttaaaaat gatgtgtgag agctttatct acaatcagac acttatgaag 1081 aagctacagg aaaccaacta cgatgtaatg cttatagacc ctgtgattcc ctgtggagac 1141 ctgatggctg agttgcttgc agtccctttt gtgctcacac ttagaatttc tgtaggaggc 1201 aatatggagc gaagctgtgg gaaacttcca gctccacttt cctatgtacc tgtgcctatg 1261 acaggactaa cagacagaat gacctttctg gaaagagtaa aaaattcaat gctttcagtt 1321 ttgttccact tctggattca ggattacgac tatcattttt gggaagagtt ttatagtaag 1381 gcattaggaa ggcccactac attatgtgag actgtgggaa aagctgagat atggctaata 1441 cgaacatatt gggattttga atttcctcaa ccataccaac ctaactttga gtttgttgga 1501 ggattgcact gtaaacctgc caaagctttg cctaaggaaa tggaaaattt tgtccagagt 1561 tcaggggaag atggtattgt ggtgttttct ctggggtcac tgtttcaaaa tgttacagaa 1621 gaaaaggcta atatcattgc ttcagccctt gcccagatcc cacagaaggt gttatggagg 1681 tacaaaggaa aaaaaccatc cacattagga gccaatactc ggctgtatga ttggataccc 1741 cagaatgatc ttcttggtca tcccaaaacc aaagctttta tcactcatgg tggaatgaat 1801 gggatctatg aagctattta ccatggggtc cctatggtgg gagttcccat atttggtgat 1861 cagcttgata acatagctca catgaaggcc aaaggagcag ctgtagaaat aaacttcaaa 1921 actatgacaa gcgaagattt actgagggct ttgagaacag tcattaccga ttcctcttat 1981 aaagagaatg ctatgagatt atcaagaatt caccatgatc aacctgtaaa gcccctagat 2041 cgagcagtct tctggatcga gtttgtcatg cgccacaaag gagccaagca cctgcgatca 2101 gctgcccatg acctcacctg gttccagcac tactctatag atgaaaactt gtactttcaa 2161 ggctCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2221 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 2281 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2341 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2401 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2461 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2521 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2581 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2641 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2701 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2761 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2821 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2881 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2941 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3001 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3061 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3121 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3181 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 3241 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3301 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3361 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3421 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3481 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3541 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3601 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3661 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3721 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3781 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3841 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3901 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3961 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4021 GCCTTTTGCT CACATGTT //