LOCUS UGT2A3-pGEc2-DES 6691 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6169..6298) /note="3'-ITR" misc_feature complement(5867..6081) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5319..5860 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4498..4520 /note="attB2" misc_feature 5298..5303 /note="BstB1 site" misc_feature 4521..5216 /note="Super GFP" misc_feature 5217..5267 /note="AviTag" misc_feature 5268..5291 /note="His Tag" PCR_frame 3002..4498 /note="Reading Frame" /set="Set 1" source 3006..4475 /dnas_title="UGT2A3-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:163405 IMAGE:40146564" /tissue_type="Pooled, cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus, PCR rescued clones" /clone_lib="NIH_MGC_362" /lab_host="DH10B" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AAACGGTC" gene 3006..4475 /dnas_title="UGT2A3" /gene="UGT2A3" /db_xref="GeneID:79799" /db_xref="HGNC:28528" CDS 3006..4475 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide A3" /gene="UGT2A3" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide A3" /protein_id="AAI30534.1" /db_xref="GI:120660408" /db_xref="GeneID:79799" /db_xref="HGNC:28528" /translation="MRSDKSALVFLLLQLFCVGCGFCGKVLVWPCDMSHWLNVKVILE ELIVRGHEVTVLTHSKPSLIDYRKPSALKFEVVHMPQDRTEENEIFVDLALNVLPGLS TWQSVIKLNDFFVEIRGTLKMMCESFIYNQTLMKKLQETNYDVMLIDPVIPCGDLMAE LLAVPFVLTLRISVGGNMERSCGKLPAPLSYVPVPMTGLTDRMTFLERVKNSMLSVLF HFWIQDYDYHFWEEFYSKALGRPTTLCETVGKAEIWLIRTYWDFEFPQPYQPNFEFVG GLHCKPAKALPKEMENFVQSSGEDGIVVFSLGSLFQNVTEEKANIIASALAQIPQKVL WRYKGKKPSTLGANTRLYDWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVP IFGDQLDNIAHMKAKGAAVEINFKTMTSEDLLRALRTVITDSSYKENAMRLSRIHHDQ PVKPLDRAVFWIEFVMRHKGAKHLRSAAHDLTWFQHYSIDVIGFLLTCVATAIFLFTK CFLFSCQKFNKTRKIEKRE" misc_feature 3006..4475 /note="UGT2A3 coding region" misc_difference 3404..3404 /dnas_title="UGT2A3" /gene="UGT2A3" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4476..4496 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4505) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4505) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4506..6691,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4505 /source="UGT2A3-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag gtctgacaag tcagctttgg tatttctgct cctgcagctc ttctgtgttg 3061 gctgtggatt ctgtgggaaa gtcctggtgt ggccctgtga catgagccat tggcttaatg 3121 tcaaggtcat tctagaagag ctcatagtga gaggccatga ggtaacagta ttgactcact 3181 caaagccttc gttaattgac tacaggaagc cttctgcatt gaaatttgag gtggtccata 3241 tgccacagga cagaacagaa gaaaatgaaa tatttgttga cctagctctg aatgtcttgc 3301 caggcttatc aacctggcaa tcagttataa aattaaatga tttttttgtt gaaataagag 3361 gaactttaaa aatgatgtgt gagagcttta tctacaatca gacacttatg aagaagctac 3421 aggaaaccaa ctacgatgta atgcttatag accctgtgat tccctgtgga gacctgatgg 3481 ctgagttgct tgcagtccct tttgtgctca cacttagaat ttctgtagga ggcaatatgg 3541 agcgaagctg tgggaaactt ccagctccac tttcctatgt acctgtgcct atgacaggac 3601 taacagacag aatgaccttt ctggaaagag taaaaaattc aatgctttca gttttgttcc 3661 acttctggat tcaggattac gactatcatt tttgggaaga gttttatagt aaggcattag 3721 gaaggcccac tacattatgt gagactgtgg gaaaagctga gatatggcta atacgaacat 3781 attgggattt tgaatttcct caaccatacc aacctaactt tgagtttgtt ggaggattgc 3841 actgtaaacc tgccaaagct ttgcctaagg aaatggaaaa ttttgtccag agttcagggg 3901 aagatggtat tgtggtgttt tctctggggt cactgtttca aaatgttaca gaagaaaagg 3961 ctaatatcat tgcttcagcc cttgcccaga tcccacagaa ggtgttatgg aggtacaaag 4021 gaaaaaaacc atccacatta ggagccaata ctcggctgta tgattggata ccccagaatg 4081 atcttcttgg tcatcccaaa accaaagctt ttatcactca tggtggaatg aatgggatct 4141 atgaagctat ttaccatggg gtccctatgg tgggagttcc catatttggt gatcagcttg 4201 ataacatagc tcacatgaag gccaaaggag cagctgtaga aataaacttc aaaactatga 4261 caagcgaaga tttactgagg gctttgagaa cagtcattac cgattcctct tataaagaga 4321 atgctatgag attatcaaga attcaccatg atcaacctgt aaagccccta gatcgagcag 4381 tcttctggat cgagtttgtc atgcgccaca aaggagccaa gcacctgcga tcagctgccc 4441 atgacctcac ctggttccag cactactcta tagatgaaaa cttgtacttt caaggctCCA 4501 GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA 4561 TCCTGGTGGA ACTGGATGGC GACGTGAACG GCCACAAGTT CTCTGTGCGG GGAGAGGGCG 4621 AAGGCGACGC CACAAATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 4681 CCGTGCCTTG GCCTACCCTC GTGACCACAC TGACCTACGG CGTGCAGTGC TTCAGCAGAT 4741 ACCCCGACCA TATGAAGCGG CACGACTTCT TCAAGAGCGC CATGCCCGAG GGCTACGTGC 4801 AGGAACGGAC CATCAGCTTC AAGGACGACG GCACCTACAA GACCAGAGCC GAAGTGAAGT 4861 TCGAGGGCGA CACCCTCGTG AACCGGATCG AGCTGAAGGG CATCGATTTC AAAGAGGACG 4921 GCAACATCCT GGGCCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTG TACATCACCG 4981 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC GTGGAAGATG 5041 GCAGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGAGAT GGCCCCGTGC 5101 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCGTGCT GAGCAAGGAC CCCAACGAGA 5161 AGCGGGACCA CATGGTGCTG CTGGAATTCG TGACCGCCGC TGGCATCACC CACGGCATGA 5221 GCGGCCTGAA CGACATCTTC GAGGCCCAGA AAATCGAGTG GCACGAGCAC CACCATCATC 5281 ACCATCACCA CTGATGATTC GAAAAATAAT AAGGATCCAA TCAACCTCTG GATTACAAAA 5341 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 5401 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 5461 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 5521 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 5581 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 5641 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 5701 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 5761 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 5821 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 5881 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 5941 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 6001 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 6061 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 6121 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 6181 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 6241 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 6301 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 6361 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 6421 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 6481 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 6541 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 6601 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 6661 TTTTAACAAA ATATTAACGC TTACAATTTA G //