LOCUS UGT2A3-pGEn1-DES 5983 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4611..5152 /note="WPRE" misc_recomb 4573..4595 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5159..5373) /note="BGH pA" misc_feature complement(5461..5590) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4566 /dnas_title="UGT2A3-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:163405 IMAGE:40146564" /tissue_type="Pooled, cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus, PCR rescued clones" /clone_lib="NIH_MGC_362" /lab_host="DH10B" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AAACGGTC" gene 3166..4566 /dnas_title="UGT2A3" /gene="UGT2A3" /db_xref="GeneID:79799" /db_xref="HGNC:28528" CDS 3166..4566 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide A3" /gene="UGT2A3" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide A3" /protein_id="AAI30534.1" /db_xref="GI:120660408" /db_xref="GeneID:79799" /db_xref="HGNC:28528" /translation="MRSDKSALVFLLLQLFCVGCGFCGKVLVWPCDMSHWLNVKVILE ELIVRGHEVTVLTHSKPSLIDYRKPSALKFEVVHMPQDRTEENEIFVDLALNVLPGLS TWQSVIKLNDFFVEIRGTLKMMCESFIYNQTLMKKLQETNYDVMLIDPVIPCGDLMAE LLAVPFVLTLRISVGGNMERSCGKLPAPLSYVPVPMTGLTDRMTFLERVKNSMLSVLF HFWIQDYDYHFWEEFYSKALGRPTTLCETVGKAEIWLIRTYWDFEFPQPYQPNFEFVG GLHCKPAKALPKEMENFVQSSGEDGIVVFSLGSLFQNVTEEKANIIASALAQIPQKVL WRYKGKKPSTLGANTRLYDWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVP IFGDQLDNIAHMKAKGAAVEINFKTMTSEDLLRALRTVITDSSYKENAMRLSRIHHDQ PVKPLDRAVFWIEFVMRHKGAKHLRSAAHDLTWFQHYSIDVIGFLLTCVATAIFLFTK CFLFSCQKFNKTRKIEKRE" misc_feature 3166..4566 /note="UGT2A3 coding region" misc_difference 3495..3495 /dnas_title="UGT2A3" /gene="UGT2A3" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4581..5983,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4580 /source="UGT2A3_Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgggaa agtcctggtg 3181 tggccctgtg acatgagcca ttggcttaat gtcaaggtca ttctagaaga gctcatagtg 3241 agaggccatg aggtaacagt attgactcac tcaaagcctt cgttaattga ctacaggaag 3301 ccttctgcat tgaaatttga ggtggtccat atgccacagg acagaacaga agaaaatgaa 3361 atatttgttg acctagctct gaatgtcttg ccaggcttat caacctggca atcagttata 3421 aaattaaatg atttttttgt tgaaataaga ggaactttaa aaatgatgtg tgagagcttt 3481 atctacaatc agacacttat gaagaagcta caggaaacca actacgatgt aatgcttata 3541 gaccctgtga ttccctgtgg agacctgatg gctgagttgc ttgcagtccc ttttgtgctc 3601 acacttagaa tttctgtagg aggcaatatg gagcgaagct gtgggaaact tccagctcca 3661 ctttcctatg tacctgtgcc tatgacagga ctaacagaca gaatgacctt tctggaaaga 3721 gtaaaaaatt caatgctttc agttttgttc cacttctgga ttcaggatta cgactatcat 3781 ttttgggaag agttttatag taaggcatta ggaaggccca ctacattatg tgagactgtg 3841 ggaaaagctg agatatggct aatacgaaca tattgggatt ttgaatttcc tcaaccatac 3901 caacctaact ttgagtttgt tggaggattg cactgtaaac ctgccaaagc tttgcctaag 3961 gaaatggaaa attttgtcca gagttcaggg gaagatggta ttgtggtgtt ttctctgggg 4021 tcactgtttc aaaatgttac agaagaaaag gctaatatca ttgcttcagc ccttgcccag 4081 atcccacaga aggtgttatg gaggtacaaa ggaaaaaaac catccacatt aggagccaat 4141 actcggctgt atgattggat accccagaat gatcttcttg gtcatcccaa aaccaaagct 4201 tttatcactc atggtggaat gaatgggatc tatgaagcta tttaccatgg ggtccctatg 4261 gtgggagttc ccatatttgg tgatcagctt gataacatag ctcacatgaa ggccaaagga 4321 gcagctgtag aaataaactt caaaactatg acaagcgaag atttactgag ggctttgaga 4381 acagtcatta ccgattcctc ttataaagag aatgctatga gattatcaag aattcaccat 4441 gatcaacctg taaagcccct agatcgagca gtcttctgga tcgagtttgt catgcgccac 4501 aaaggagcca agcacctgcg atcagctgcc catgacctca cctggttcca gcactactct 4561 atagatTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA GCTTGGATCC AATCAACCTC 4621 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 4681 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 4741 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 4801 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 4861 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 4921 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 4981 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 5041 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 5101 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC 5161 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 5221 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 5281 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 5341 GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA 5401 GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA 5461 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 5521 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 5581 GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA 5641 TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG 5701 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 5761 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG 5821 AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 5881 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 5941 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAG //