LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2168..2264) /label=attL2 /note="attL2 (c)" misc_signal complement(2279..2298) /note="T7 Promoter/priming site (c)" primer_bind 2306..2322 /note="M13 Reverse priming site" gene 2435..3244 /note="Kanamycin resistance gene" rep_origin 3365..4038 /note="pUC origin" PCR_frame 669..2168 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2188) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2185) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2176..4041,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2146..2166 /note="TEV site" source 673..2145 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:142209 IMAGE:8322701" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: CCCCTTGC" gene 673..2145 /gene="UGT2B10" /db_xref="GeneID:7365" /db_xref="HGNC:12544" /db_xref="MIM:600070" CDS 673..2145 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide B10" /gene="UGT2B10" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide B10" /protein_id="AAI13650.1" /db_xref="GI:109730577" /db_xref="GeneID:7365" /db_xref="HGNC:12544" /db_xref="MIM:600070" /translation="MALKWTTVLLIQLSFYFSSGSCGKVLVWAAEYSLWMNMKTILKE LVQRGHEVTVLASSASILFDPNDSSTLKLEVYPTSLTKTEFENIIMQLVKRLSEIQKD TFWLPFSQEQEILWAINDIIRNFCKDVVSNKKLMKKLQESRFDIVFADAYLPCGELLA ELFNIPFVYSHSFSPGYSFERHSGGFIFPPSYVPVVMSKLSDQMTFMERVKNMLYVLY FDFWFQIFNMKKWDQFYSEVLGRPTTLSETMRKADIWLMRNSWNFKFPHPFLPNVDFV GGLHCKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNMTEERANVIATALAKIPQKV LWRFDGNKPDALGLNTRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGI PLFFDQPDNIAHMKAKGAAVRVDFNTMSSTDLLNALKTVINDPSYKENIMKLSRIQHD QPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWFQYHSLDVIGFLLACVATVLFIIT KCCLFCFWKFARKGKKGKRD" misc_feature 673..2145 /note="UGT2B10 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggctct gaaatggact acagttctgc tgatacaact cagtttttac 721 tttagctctg ggagttgtgg aaaggtgctg gtatgggccg cagaatacag cctttggatg 781 aatatgaaga caatcctgaa agaacttgtt cagagaggtc atgaggtgac tgtactggca 841 tcttcagctt ccattctttt tgatcccaac gactcatcca ctcttaaact tgaagtttat 901 cctacatctt taactaaaac tgaatttgag aatatcatca tgcaattggt taagagattg 961 tcagaaattc aaaaagatac attttggtta cctttttcac aagaacaaga aatcctgtgg 1021 gcaattaatg acataattag aaacttctgt aaagatgtag tttcaaataa gaaacttatg 1081 aaaaaactac aagagtcaag atttgacatc gtttttgcag atgcttattt accctgtggt 1141 gagctgctgg ctgagctatt taacataccc tttgtgtaca gtcacagctt cagtcctggc 1201 tactcatttg aaaggcacag tggaggattt attttccctc cttcctacgt acctgttgtt 1261 atgtcaaaat taagtgatca aatgactttc atggagaggg taaaaaatat gctctatgtg 1321 ctttattttg acttttggtt ccaaatattt aatatgaaga agtgggatca gttttacagt 1381 gaagttttag gaagacccac tacattatct gagacaatga ggaaagctga catatggctt 1441 atgcgaaact cctggaattt taaatttcct catccattct taccaaatgt tgattttgtt 1501 ggaggactcc actgcaaacc tgccaaaccc ctacctaagg aaatggagga gtttgtacag 1561 agctctggag aaaatggtgt tgtggtgttt tctctggggt caatggtcag taacatgaca 1621 gaagaaaggg ccaacgtaat tgcaacagcc cttgccaaga tcccacaaaa ggttctttgg 1681 agatttgatg ggaataaacc agatgcctta ggtctcaata ctcgactgta caagtggata 1741 ccccagaatg accttctagg tcatccaaaa accagagctt ttataactca tggtggagcc 1801 aatggcatct atgaggcaat ctaccatggg atccctatgg tgggcattcc attgtttttt 1861 gatcaacctg ataatattgc tcacatgaag gccaagggag cagctgttag agtggacttc 1921 aacacaatgt cgagtacaga cctgctgaat gcactgaaga cagtaattaa tgatccttca 1981 tataaagaga atattatgaa attatcaaga attcaacatg atcaaccagt gaagcccctg 2041 gatcgagcag tcttctggat tgaatttgtc atgcgccaca aaggagccaa acatcttcga 2101 gttgcagccc acaacctcac ctggttccag taccactctt tggatgaaaa cttgtacttt 2161 caaggctCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 2221 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 2281 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 2341 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT 2401 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 2461 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 2521 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 2581 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 2641 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 2701 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 2761 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 2821 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 2881 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 2941 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 3001 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 3061 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 3121 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 3181 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 3241 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 3301 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 3361 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3421 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3481 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 3541 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3601 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3661 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 3721 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 3781 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 3841 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 3901 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 3961 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4021 CTGGCCTTTT GCTCACATGT T //