LOCUS UGT2B10-pGEc2-DE 6694 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6172..6301) /note="3'-ITR" misc_feature complement(5870..6084) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5322..5863 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4501..4523 /note="attB2" misc_feature 5301..5306 /note="BstB1 site" misc_feature 4524..5219 /note="Super GFP" misc_feature 5220..5270 /note="AviTag" misc_feature 5271..5294 /note="His Tag" PCR_frame 3002..4501 /note="Reading Frame" /set="Set 1" source 3006..4478 /dnas_title="UGT2B10-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:142209 IMAGE:8322701" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: CCCCTTGC" gene 3006..4478 /dnas_title="UGT2B10" /gene="UGT2B10" /db_xref="GeneID:7365" /db_xref="HGNC:12544" /db_xref="MIM:600070" CDS 3006..4478 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide B10" /gene="UGT2B10" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide B10" /protein_id="AAI13650.1" /db_xref="GI:109730577" /db_xref="GeneID:7365" /db_xref="HGNC:12544" /db_xref="MIM:600070" /translation="MALKWTTVLLIQLSFYFSSGSCGKVLVWAAEYSLWMNMKTILKE LVQRGHEVTVLASSASILFDPNDSSTLKLEVYPTSLTKTEFENIIMQLVKRLSEIQKD TFWLPFSQEQEILWAINDIIRNFCKDVVSNKKLMKKLQESRFDIVFADAYLPCGELLA ELFNIPFVYSHSFSPGYSFERHSGGFIFPPSYVPVVMSKLSDQMTFMERVKNMLYVLY FDFWFQIFNMKKWDQFYSEVLGRPTTLSETMRKADIWLMRNSWNFKFPHPFLPNVDFV GGLHCKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNMTEERANVIATALAKIPQKV LWRFDGNKPDALGLNTRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGI PLFFDQPDNIAHMKAKGAAVRVDFNTMSSTDLLNALKTVINDPSYKENIMKLSRIQHD QPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWFQYHSLDVIGFLLACVATVLFIIT KCCLFCFWKFARKGKKGKRD" misc_feature 3006..4478 /note="UGT2B10 coding region" misc_feature 4479..4499 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4508) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4508) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4509..6694,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4508 /source="UGT2B10-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc tctgaaatgg actacagttc tgctgataca actcagtttt tactttagct 3061 ctgggagttg tggaaaggtg ctggtatggg ccgcagaata cagcctttgg atgaatatga 3121 agacaatcct gaaagaactt gttcagagag gtcatgaggt gactgtactg gcatcttcag 3181 cttccattct ttttgatccc aacgactcat ccactcttaa acttgaagtt tatcctacat 3241 ctttaactaa aactgaattt gagaatatca tcatgcaatt ggttaagaga ttgtcagaaa 3301 ttcaaaaaga tacattttgg ttaccttttt cacaagaaca agaaatcctg tgggcaatta 3361 atgacataat tagaaacttc tgtaaagatg tagtttcaaa taagaaactt atgaaaaaac 3421 tacaagagtc aagatttgac atcgtttttg cagatgctta tttaccctgt ggtgagctgc 3481 tggctgagct atttaacata ccctttgtgt acagtcacag cttcagtcct ggctactcat 3541 ttgaaaggca cagtggagga tttattttcc ctccttccta cgtacctgtt gttatgtcaa 3601 aattaagtga tcaaatgact ttcatggaga gggtaaaaaa tatgctctat gtgctttatt 3661 ttgacttttg gttccaaata tttaatatga agaagtggga tcagttttac agtgaagttt 3721 taggaagacc cactacatta tctgagacaa tgaggaaagc tgacatatgg cttatgcgaa 3781 actcctggaa ttttaaattt cctcatccat tcttaccaaa tgttgatttt gttggaggac 3841 tccactgcaa acctgccaaa cccctaccta aggaaatgga ggagtttgta cagagctctg 3901 gagaaaatgg tgttgtggtg ttttctctgg ggtcaatggt cagtaacatg acagaagaaa 3961 gggccaacgt aattgcaaca gcccttgcca agatcccaca aaaggttctt tggagatttg 4021 atgggaataa accagatgcc ttaggtctca atactcgact gtacaagtgg ataccccaga 4081 atgaccttct aggtcatcca aaaaccagag cttttataac tcatggtgga gccaatggca 4141 tctatgaggc aatctaccat gggatcccta tggtgggcat tccattgttt tttgatcaac 4201 ctgataatat tgctcacatg aaggccaagg gagcagctgt tagagtggac ttcaacacaa 4261 tgtcgagtac agacctgctg aatgcactga agacagtaat taatgatcct tcatataaag 4321 agaatattat gaaattatca agaattcaac atgatcaacc agtgaagccc ctggatcgag 4381 cagtcttctg gattgaattt gtcatgcgcc acaaaggagc caaacatctt cgagttgcag 4441 cccacaacct cacctggttc cagtaccact ctttggatga aaacttgtac tttcaaggct 4501 CCAGCTTTCT TGTACAAAGT GGTATGAGCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC 4561 CCATCCTGGT GGAACTGGAT GGCGACGTGA ACGGCCACAA GTTCTCTGTG CGGGGAGAGG 4621 GCGAAGGCGA CGCCACAAAT GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC 4681 TGCCCGTGCC TTGGCCTACC CTCGTGACCA CACTGACCTA CGGCGTGCAG TGCTTCAGCA 4741 GATACCCCGA CCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACG 4801 TGCAGGAACG GACCATCAGC TTCAAGGACG ACGGCACCTA CAAGACCAGA GCCGAAGTGA 4861 AGTTCGAGGG CGACACCCTC GTGAACCGGA TCGAGCTGAA GGGCATCGAT TTCAAAGAGG 4921 ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTGTACATCA 4981 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGGCAC AACGTGGAAG 5041 ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAACAC CCCCATCGGA GATGGCCCCG 5101 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGAGCGT GCTGAGCAAG GACCCCAACG 5161 AGAAGCGGGA CCACATGGTG CTGCTGGAAT TCGTGACCGC CGCTGGCATC ACCCACGGCA 5221 TGAGCGGCCT GAACGACATC TTCGAGGCCC AGAAAATCGA GTGGCACGAG CACCACCATC 5281 ATCACCATCA CCACTGATGA TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA 5341 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 5401 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 5461 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 5521 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 5581 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 5641 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 5701 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 5761 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 5821 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT 5881 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 5941 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 6001 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 6061 AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC 6121 TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 6181 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 6241 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 6301 GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA 6361 TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 6421 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 6481 ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 6541 TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 6601 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 6661 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //