LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2174..2270) /label=attL2 /note="attL2 (c)" misc_signal complement(2285..2304) /note="T7 Promoter/priming site (c)" primer_bind 2312..2328 /note="M13 Reverse priming site" gene 2441..3250 /note="Kanamycin resistance gene" rep_origin 3371..4044 /note="pUC origin" PCR_frame 669..2174 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2194) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2191) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2182..4047,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2152..2172 /note="TEV site" source 673..2151 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:180134 IMAGE:100014811" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 673..2151 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 673..2151 /gene="UGT2B15" /gene_synonym=UGT2B8 /db_xref="GeneID:7366" /db_xref="HGNC:12546" /db_xref="MIM:600069" CDS 673..2151 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide B15" /gene="UGT2B15" /gene_synonym=UGT2B8 /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide B15" /protein_id="AAI46571.1" /db_xref="GI:148922096" /db_xref="GeneID:7366" /db_xref="HGNC:12546" /db_xref="MIM:600069" /translation="MSLKWTSVFLLIQLSCYFSSGSCGKVLVWPTEYSHWINMKTILE ELVQRGHEVTVLTSSASTLVNASKSSAIKLEVYPTSLTKNYLEDSLLKILDRWIYGVS KNTFWSYFSQLQELCWEYYDYSNKLCKDAVLNKKLMMKLQESKFDVILADALNPCGEL LAELFNIPFLYSLRFSVGYTFEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIHM LYFDFWFQIYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVD FVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQ KVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMV GIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPVYKENVMKLSRIH HDQPMKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATVIFI ITKFCLFCFRKLAKKGKKKKRD" misc_feature 673..2151 /note="UGT2B15 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtctct gaaatggacg tcagtctttc tgctgataca gctcagttgt 721 tactttagct ctggaagctg tggaaaggtg ctagtgtggc ccacagaata cagccattgg 781 ataaatatga agacaatcct ggaagagctt gttcagaggg gtcatgaggt gactgtgttg 841 acatcttcgg cttctactct tgtcaatgcc agtaaatcat ctgctattaa attagaagtt 901 tatcctacat ctttaactaa aaattatttg gaagattctc ttctgaaaat tctcgataga 961 tggatatatg gtgtttcaaa aaatacattt tggtcatatt tttcacaatt acaagaattg 1021 tgttgggaat attatgacta cagtaacaag ctctgtaaag atgcagtttt gaataagaaa 1081 cttatgatga aactacaaga gtcaaagttt gatgtcattc tggcagatgc ccttaatccc 1141 tgtggtgagc tactggctga actatttaac ataccctttc tgtacagtct tcgattctct 1201 gttggctaca catttgagaa gaatggtgga ggatttctgt tccctccttc ctatgtacct 1261 gttgttatgt cagaattaag tgatcaaatg attttcatgg agaggataaa aaatatgata 1321 catatgcttt attttgactt ttggtttcaa atttatgatc tgaagaagtg ggaccagttt 1381 tatagtgaag ttctaggaag acccactaca ttatttgaga caatggggaa agctgaaatg 1441 tggctcattc gaacctattg ggattttgaa tttcctcgcc cattcttacc aaatgttgat 1501 tttgttggag gacttcactg taaaccagcc aaacccctgc ctaaggaaat ggaagagttt 1561 gtgcagagct ctggagaaaa tggtattgtg gtgttttctc tggggtcgat gatcagtaac 1621 atgtcagaag aaagtgccaa catgattgca tcagcccttg cccagatccc acaaaaggtt 1681 ctatggagat ttgatggcaa gaagccaaat actttaggtt ccaatactcg actgtacaag 1741 tggttacccc agaatgacct tcttggtcat cccaaaacca aagcttttat aactcatggt 1801 ggaaccaatg gcatctatga ggcgatctac catgggatcc ctatggtggg cattcccttg 1861 tttgcggatc aacatgataa cattgctcac atgaaagcca agggagcagc cctcagtgtg 1921 gacatcagga ccatgtcaag tagagatttg ctcaatgcat tgaagtcagt cattaatgac 1981 cctgtctata aagagaatgt catgaaatta tcaagaattc atcatgacca accaatgaag 2041 cccctggatc gagcagtctt ctggattgag tttgtcatgc gccacaaagg agccaagcac 2101 cttcgagtcg cagctcacaa cctcacctgg atccagtacc actctttgga tgaaaacttg 2161 tactttcaag gctCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 2221 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 2281 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 2341 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2401 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2461 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2521 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2581 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2641 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2701 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2761 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2821 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2881 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2941 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 3001 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 3061 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 3121 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 3181 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 3241 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 3301 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3361 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3421 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3481 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3541 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3601 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3661 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3721 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3781 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3841 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3901 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3961 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 4021 CTTTTGCTGG CCTTTTGCTC ACATGTT //