LOCUS UGT2B15-pGEn1-DE 5992 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4620..5161 /note="WPRE" misc_recomb 4582..4604 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5168..5382) /note="BGH pA" misc_feature complement(5470..5599) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4575 /dnas_title="UGT2B15-pGEn1-DEST" /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:180134 IMAGE:100014811" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 3166..4575 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 3166..4575 /dnas_title="UGT2B15" /gene="UGT2B15" /gene_synonym="UGT2B8" /db_xref="GeneID:7366" /db_xref="HGNC:12546" /db_xref="MIM:600069" CDS 3166..4575 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide B15" /gene="UGT2B15" /gene_synonym="UGT2B8" /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide B15" /protein_id="AAI46571.1" /db_xref="GI:148922096" /db_xref="GeneID:7366" /db_xref="HGNC:12546" /db_xref="MIM:600069" /translation="MSLKWTSVFLLIQLSCYFSSGSCGKVLVWPTEYSHWINMKTILE ELVQRGHEVTVLTSSASTLVNASKSSAIKLEVYPTSLTKNYLEDSLLKILDRWIYGVS KNTFWSYFSQLQELCWEYYDYSNKLCKDAVLNKKLMMKLQESKFDVILADALNPCGEL LAELFNIPFLYSLRFSVGYTFEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIHM LYFDFWFQIYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVD FVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQ KVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMV GIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPVYKENVMKLSRIH HDQPMKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATVIFI ITKFCLFCFRKLAKKGKKKKRD" misc_feature 3166..4575 /note="UGT2B15 coding region" vector join(4590..5992,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4589 /source="UGT2B15_Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcggaaa ggtgctagtg 3181 tggcccacag aatacagcca ttggataaat atgaagacaa tcctggaaga gcttgttcag 3241 aggggtcatg aggtgactgt gttgacatct tcggcttcta ctcttgtcaa tgccagtaaa 3301 tcatctgcta ttaaattaga agtttatcct acatctttaa ctaaaaatta tttggaagat 3361 tctcttctga aaattctcga tagatggata tatggtgttt caaaaaatac attttggtca 3421 tatttttcac aattacaaga attgtgttgg gaatattatg actacagtaa caagctctgt 3481 aaagatgcag ttttgaataa gaaacttatg atgaaactac aagagtcaaa gtttgatgtc 3541 attctggcag atgcccttaa tccctgtggt gagctactgg ctgaactatt taacataccc 3601 tttctgtaca gtcttcgatt ctctgttggc tacacatttg agaagaatgg tggaggattt 3661 ctgttccctc cttcctatgt acctgttgtt atgtcagaat taagtgatca aatgattttc 3721 atggagagga taaaaaatat gatacatatg ctttattttg acttttggtt tcaaatttat 3781 gatctgaaga agtgggacca gttttatagt gaagttctag gaagacccac tacattattt 3841 gagacaatgg ggaaagctga aatgtggctc attcgaacct attgggattt tgaatttcct 3901 cgcccattct taccaaatgt tgattttgtt ggaggacttc actgtaaacc agccaaaccc 3961 ctgcctaagg aaatggaaga gtttgtgcag agctctggag aaaatggtat tgtggtgttt 4021 tctctggggt cgatgatcag taacatgtca gaagaaagtg ccaacatgat tgcatcagcc 4081 cttgcccaga tcccacaaaa ggttctatgg agatttgatg gcaagaagcc aaatacttta 4141 ggttccaata ctcgactgta caagtggtta ccccagaatg accttcttgg tcatcccaaa 4201 accaaagctt ttataactca tggtggaacc aatggcatct atgaggcgat ctaccatggg 4261 atccctatgg tgggcattcc cttgtttgcg gatcaacatg ataacattgc tcacatgaaa 4321 gccaagggag cagccctcag tgtggacatc aggaccatgt caagtagaga tttgctcaat 4381 gcattgaagt cagtcattaa tgaccctgtc tataaagaga atgtcatgaa attatcaaga 4441 attcatcatg accaaccaat gaagcccctg gatcgagcag tcttctggat tgagtttgtc 4501 atgcgccaca aaggagccaa gcaccttcga gtcgcagctc acaacctcac ctggatccag 4561 taccactctt tggatTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAG CTTGGATCCA 4621 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 4681 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 4741 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 4801 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 4861 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 4921 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 4981 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 5041 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 5101 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 5161 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 5221 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 5281 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 5341 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 5401 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 5461 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 5521 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 5581 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 5641 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 5701 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 5761 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 5821 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 5881 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 5941 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //