LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2171..2267) /label=attL2 /note="attL2 (c)" misc_signal complement(2282..2301) /note="T7 Promoter/priming site (c)" primer_bind 2309..2325 /note="M13 Reverse priming site" gene 2438..3247 /note="Kanamycin resistance gene" rep_origin 3368..4041 /note="pUC origin" PCR_frame 669..2171 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2191) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2188) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2179..4044,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2149..2169 /note="TEV site" source 673..2148 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:22623 IMAGE:4709436" /tissue_type="Liver" /clone_lib="NIH_MGC_76" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 673..2148 /gene="UGT2B4" /gene_synonym=UGT2B11 /db_xref="GeneID:7363" /db_xref="HGNC:12553" /db_xref="MIM:600067" CDS 673..2148 /dnas_title="UDP glucuronosyltransferase 2 family, polypeptide B4" /gene="UGT2B4" /gene_synonym=UGT2B11 /codon_start=1 /product="UDP glucuronosyltransferase 2 family, polypeptide B4" /protein_id="AAH26264.1" /db_xref="GI:20070776" /db_xref="GeneID:7363" /db_xref="HGNC:12553" /db_xref="MIM:600067" /translation="MSMKWTSALLLIQLSCYFSSGSCGKVLVWPTEFSHWMNIKTILD ELVQRGHEVTVLASSASISFDPNSPSTLKFEVYPVSLTKTEFEDIIKQLVKRWAELPK DTFWSYFSQVQEIMWTFNDILRKFCKDIVSNKKLMKKLQESRFDVVLADAVFPFGELL AELLKIPFVYSLRFSPGYAIEKHSGGLLFPPSYVPVVMSELSDQMTFIERVKNMIYVL YFEFWFQIFDMKKWDQFYSEVLGRPTTLSETMAKADIWLIRNYWDFQFPHPLLPNVEF VGGLHCKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNTSEERANVIASALAKIPQK VLWRFDGNKPDTLGLNTRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVG VPLFADQPDNIAHMKAKGAAVSLDFHTMSSTDLLNALKTVINDPLYKENAMKLSRIHH DQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHDLTWFQYHSLDVTGFLLACVATVIFII TKCLFCVWKFVRTGKKGKRD" misc_feature 673..2148 /note="UGT2B4 coding region" misc_difference 1620..1620 /gene="UGT2B4" /gene_synonym=UGT2B11 /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 1941..1941 /gene="UGT2B4" /gene_synonym=UGT2B11 /note="'T' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtctat gaaatggact tcagctcttc tgctgataca gctgagctgt 721 tactttagct ctgggagttg tggaaaggtg ctggtgtggc ccacagaatt cagccactgg 781 atgaatataa agacaatcct ggatgaactt gtccagagag gtcatgaggt gactgtattg 841 gcatcttcag cttccatttc tttcgatccc aacagcccat ctactcttaa atttgaagtt 901 tatcctgtat ctttaactaa aactgagttt gaggatatta tcaagcagct ggttaagaga 961 tgggcagaac ttccaaaaga cacattttgg tcatattttt cacaagtaca agaaatcatg 1021 tggacattta atgacatact tagaaagttc tgtaaggata tagtttcaaa taagaaactt 1081 atgaagaaac tacaggagtc aagatttgat gttgttcttg cagatgctgt tttccccttt 1141 ggtgagctgc tggccgagtt acttaaaata ccctttgtct acagcctccg cttctctcct 1201 ggctacgcaa ttgaaaagca tagtggagga cttctgttcc ctccttccta tgtgcctgtt 1261 gttatgtcag aactaagtga ccaaatgact ttcatagaga gggtaaaaaa tatgatctat 1321 gtgctttatt ttgaattttg gttccaaata tttgacatga agaagtggga tcagttctac 1381 agtgaagttc taggaagacc cactacgtta tctgagacaa tggcaaaagc tgacatatgg 1441 cttattcgaa actactggga ttttcaattt cctcacccac tcttaccaaa tgttgagttc 1501 gttggaggac tccactgcaa acctgccaaa cccctaccga aggaaatgga agagtttgtc 1561 cagagctctg gagaaaatgg tgttgtggtg ttttctctgg ggtcgatggt cagtaacacg 1621 tcagaagaaa gggccaatgt aattgcatca gcccttgcca agatcccaca aaaggttctg 1681 tggagatttg atgggaataa accagatact ttaggactca atactcggct gtacaagtgg 1741 ataccccaga atgatcttct tggtcaccca aaaaccagag cttttataac tcatggtgga 1801 gccaatggca tctatgaggc aatctaccat ggaatcccta tggtgggcgt tccattgttt 1861 gcagatcaac ctgataacat tgcacacatg aaggccaagg gagcagctgt tagtttggac 1921 ttccacacaa tgtcgagtac tgacttactc aatgcactga agacagtaat taatgatcct 1981 ttatataaag agaatgctat gaaattatca agaattcatc atgatcaacc agtgaagccc 2041 cttgatcgag cagtcttctg gattgaattt gtcatgcgcc ataaaggagc caagcacctt 2101 cgggttgcag cccacgacct cacctggttc cagtaccact ctttggatga aaacttgtac 2161 tttcaaggct CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 2221 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA 2281 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT 2341 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA 2401 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC 2461 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG 2521 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC 2581 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT 2641 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC 2701 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT 2761 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG 2821 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC 2881 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG 2941 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC 3001 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 3061 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC 3121 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA 3181 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT 3241 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG 3301 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC 3361 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3421 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3481 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3541 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3601 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3661 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3721 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 3781 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 3841 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 3901 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 3961 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4021 TTGCTGGCCT TTTGCTCACA TGTT //