LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2066..2162) /label=attL2 /note="attL2 (c)" misc_signal complement(2177..2196) /note="T7 Promoter/priming site (c)" primer_bind 2204..2220 /note="M13 Reverse priming site" gene 2333..3142 /note="Kanamycin resistance gene" rep_origin 3263..3936 /note="pUC origin" vector join(2074..3939,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2059 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:104134 IMAGE:30915590" /tissue_type="Brain, PCR rescued clones" /clone_lib="NIH_MGC_272" /lab_host="DH10B" /note="Vector: pCR4 Topo TA with reversed insert" gene 692..2059 /gene="UGT8" /db_xref="GeneID:7368" /db_xref="HGNC:12555" /db_xref="MIM:601291" CDS 692..2059 /dnas_title="UGT8 protein" /gene="UGT8" /codon_start=1 /product="UGT8 protein" /protein_id="AAH75069.1" /db_xref="GI:50959968" /db_xref="GeneID:7368" /db_xref="HGNC:12555" /db_xref="MIM:601291" /translation="MKSYTPYFILLWSAVGIAKAAKIIIVPPIMFESHMYIFKTLASA LHERGHHTVFLLSEGRDIAPSNHYSLQRYPGIFNSTTSDAFLQSKMRNIFSGRLTAIE LFDILDHYTKNCDLMVGNHALIQGLKKEKFDLLLVDPNDMCGFVIAHLLGVKYAVFST GLWYPAEVGAPAPLAYVPEFNSLLTDRMNLLQRMKNTGVYLISRLGVSFLVLPKYERI MQKYNLLPEKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDL QRWVNGANEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGPKPKNLGNN TKLIEWLPQNDLLGHSKIKAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQA KGMGILLEWKTVTEKELYEALVKVINNPSYRQRAQKLSEIHKDQPGHPVNRTIYWIDY IIRHNGAHHLRAAVHQISFCQYFLLDIAFVLLLGAALLYFLLSWVTKFIYRKIKSLWS RNKHSTVNGHYHNGILNGKYKRNGHIKHEKKVK" misc_feature 692..2059 /note="UGT8 coding region" misc_difference 1309..1309 /gene="UGT8" /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 1735..1735 /gene="UGT8" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'M' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgccaaaatc atcatcgtgc cgccaattat 721 gtttgaaagc catatgtaca ttttcaagac gctagcctca gccttgcacg agagaggcca 781 ccatacagtg ttcctcctct ctgaaggcag agacatcgcc ccatctaatc attacagcct 841 ccagcgctac ccagggatct ttaacagtac cacctcagat gctttcctac agtccaagat 901 gcggaatatt ttctctggga gattgacagc aatcgaactg tttgacatac tggatcacta 961 tactaagaac tgtgacctga tggttggcaa ccatgccctg atccagggtc tgaagaaaga 1021 aaaatttgac ctgctgctgg tggaccctaa tgatatgtgt ggatttgtga tagctcatct 1081 tttaggggtt aaatatgctg tattttcaac tggcctttgg tatcctgctg aagtgggtgc 1141 tcctgctcca ttagcatacg tcccagagtt taactcactc ctcacagacc gcatgaactt 1201 gctgcaaagg atgaaaaata ccggtgttta cctcatttcc agattagggg tcagctttct 1261 ggttcttccc aaatatgaaa ggataatgca gaagtacaac ctgctgccgg agaagtccat 1321 gtatgatttg gttcatgggt ccagcctgtg gatgctgtgt actgacgtag cactggaatt 1381 cccaagaccc actctgccta atgttgttta tgtaggagga atcctaacca aaccagccag 1441 cccactacca gaagatctcc aaagatgggt aaatggtgct aatgaacatg gctttgtctt 1501 ggtgtctttt ggagctggtg tcaagtatct gtcagaagac attgctaaca aactggcagg 1561 agctctgggg agattgcctc aaaaagtgat ttggaggttt tctggaccca aaccaaagaa 1621 tctaggaaac aacactaaac tcatagaatg gttaccacaa aatgacctgc ttgggcattc 1681 aaagattaaa gccttcctga gccatggtgg tttgaacagt atttttgaaa ctatgtatca 1741 tggtgtgcct gtagtgggaa ttccactctt tggagaccat tatgatacta tgaccagagt 1801 acaggcaaaa ggcatgggga tattgctaga atggaagaca gttactgaaa aagagctcta 1861 tgaagcacta gtgaaggtta tcaataatcc cagctaccgt cagagggctc agaagctttc 1921 ggaaattcac aaggatcaac ctggtcaccc tgtcaatcga actatctatt ggatagatta 1981 tattattcgt cacaatggag cccatcacct acgtgccgct gtccatcaga tctccttttg 2041 tcagtatttt ttactggatT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2101 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2161 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2221 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2281 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2341 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2401 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2461 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2521 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2581 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2641 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2701 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2761 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2821 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2881 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2941 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3001 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3061 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3121 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3181 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3241 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3301 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3361 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3421 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3481 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3541 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3601 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3661 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3721 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3781 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3841 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3901 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //