LOCUS DPM1-pGEc2-DEST 6001 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5479..5608) /note="3'-ITR" misc_feature complement(5177..5391) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4629..5170 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3808..3830 /note="attB2" misc_feature 4608..4613 /note="BstB1 site" misc_feature 3831..4526 /note="Super GFP" misc_feature 4527..4577 /note="AviTag" misc_feature 4578..4601 /note="His Tag" PCR_frame 3002..3808 /note="Reading Frame" /set="Set 1" source 3006..3785 /dnas_title="DPM1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:12544 IMAGE:3997453" /tissue_type="Bladder, carcinoma" /clone_lib="NIH_MGC_53" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 3006..3785 /dnas_title="DPM1" /gene="DPM1" /gene_synonym="CDGIE" /gene_synonym="MPDS" /db_xref="GeneID:8813" /db_xref="HGNC:3005" /db_xref="MIM:603503" CDS 3006..3785 /dnas_title="dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit" /gene="DPM1" /gene_synonym="CDGIE" /gene_synonym="MPDS" /codon_start=1 /product="dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit" /protein_id="AAH07073.1" /db_xref="GI:13937927" /db_xref="GeneID:8813" /db_xref="HGNC:3005" /db_xref="MIM:603503" /translation="MASLEVSRSPRRSRRELEVRSPRQNKYSVLLPTYNERENLPLIV WLLVKSFSESGINYEIIIIDDGSPDGTRDVAEQLEKIYGSDRILLRPREKKLGLGTAY IHGMKHATGNYIIIMDADLSHHPKFIPEFIRKQKEGNFDIVSGTRYKGNGGVYGWDLK RKIISRGANFLTQILLRPGASDLTGSFRLYRKEVLEKLIEKCVSKGYVFQMEMIVRAR QLNYTIGEVPISFVDRVYGESKLGGNEIVSFLKGLLTLFATT" misc_feature 3006..3785 /note="DPM1 coding region" misc_feature 3786..3806 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..3815) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..3815) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(3816..6001,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..3815 /source="DPM1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ctccttggaa gtcagtcgta gtcctcgcag gtctcggcgg gagctggaag 3061 tgcgcagtcc acgacagaac aaatattcgg tgcttttacc tacctacaac gagcgcgaga 3121 acctgccgct catcgtgtgg ctgctggtga aaagcttctc cgagagtgga atcaactatg 3181 aaattataat catagatgat ggaagcccag atggaacaag ggatgttgct gaacagttgg 3241 agaagatcta tgggtcagac agaattcttc taagaccacg agagaaaaag ttgggactag 3301 gaactgcata tattcatgga atgaaacatg ccacaggaaa ctacatcatt attatggatg 3361 ctgatctctc acaccatcca aaatttattc ctgaatttat taggaagcaa aaggagggta 3421 attttgatat tgtctctgga actcgctaca aaggaaatgg aggtgtatat ggctgggatt 3481 tgaaaagaaa aataatcagc cgtggggcca attttttaac tcagatcttg ctgagaccag 3541 gagcatctga tttaacagga agtttcagat tataccgaaa agaagttcta gagaaattaa 3601 tagaaaaatg tgtttctaaa ggctacgtct tccagatgga gatgattgtt cgggcaagac 3661 agttgaatta tactattggc gaggttccaa tatcatttgt ggatcgtgtt tatggtgaat 3721 ccaagttggg aggaaatgaa atagtatctt tcttgaaagg attattgact ctttttgcta 3781 ctacagaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG 3841 GCGAGGAACT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA ACTGGATGGC GACGTGAACG 3901 GCCACAAGTT CTCTGTGCGG GGAGAGGGCG AAGGCGACGC CACAAATGGC AAGCTGACCC 3961 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCTTG GCCTACCCTC GTGACCACAC 4021 TGACCTACGG CGTGCAGTGC TTCAGCAGAT ACCCCGACCA TATGAAGCGG CACGACTTCT 4081 TCAAGAGCGC CATGCCCGAG GGCTACGTGC AGGAACGGAC CATCAGCTTC AAGGACGACG 4141 GCACCTACAA GACCAGAGCC GAAGTGAAGT TCGAGGGCGA CACCCTCGTG AACCGGATCG 4201 AGCTGAAGGG CATCGATTTC AAAGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA 4261 ACTTCAACAG CCACAACGTG TACATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA 4321 ACTTCAAGAT CCGGCACAAC GTGGAAGATG GCAGCGTGCA GCTGGCCGAC CACTACCAGC 4381 AGAACACCCC CATCGGAGAT GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 4441 AGAGCGTGCT GAGCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTCG 4501 TGACCGCCGC TGGCATCACC CACGGCATGA GCGGCCTGAA CGACATCTTC GAGGCCCAGA 4561 AAATCGAGTG GCACGAGCAC CACCATCATC ACCATCACCA CTGATGATTC GAAAAATAAT 4621 AAGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 4681 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 4741 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 4801 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 4861 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 4921 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 4981 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT 5041 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 5101 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 5161 CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 5221 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 5281 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 5341 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ACTCGAGTTA 5401 AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG TAGCATGGCG 5461 GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG 5521 CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG 5581 CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG CGCCTAGGCC 5641 TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC TCACTGGCCG 5701 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 5761 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA ACGCCCTTCC 5821 CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC TCAACCCTAT 5881 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 5941 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA 6001 G //