LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2906..3002) /label=attL2 /note="attL2 (c)" misc_signal complement(3017..3036) /note="T7 Promoter/priming site (c)" primer_bind 3044..3060 /note="M13 Reverse priming site" gene 3173..3982 /note="Kanamycin resistance gene" rep_origin 4103..4776 /note="pUC origin" PCR_frame 669..2906 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2926) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2923) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2914..4779,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2884..2904 /note="TEV site" source 673..2883 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2986 IMAGE:3143019" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2883 /gene="GYS1" /gene_synonym=GSY /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" CDS 673..2883 /dnas_title="glycogen synthase 1 (muscle)" /gene="GYS1" /gene_synonym=GSY /codon_start=1 /product="glycogen synthase 1 (muscle)" /protein_id="AAH02617.1" /db_xref="GI:12803569" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" /translation="MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIY TVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVY FGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAV DFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDI VTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEF SNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDT ANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSY YEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQ LTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYE PNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAA KDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN" misc_feature 673..2883 /note="GYS1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgccttt aaaccgcact ttgtccatgt cctcactgcc aggactggag 721 gactgggagg atgaattcga cctggagaac gcagtgctct tcgaagtggc ctgggaggtg 781 gctaacaagg tgggtggcat ctacacggtg ctgcagacga aggcgaaggt gacaggggac 841 gaatggggcg acaactactt cctggtgggg ccgtacacgg agcagggcgt gaggacccag 901 gtggaactgc tggaggcccc caccccggcc ctgaagagga cactggattc catgaacagc 961 aagggctgca aggtgtattt cgggcgctgg ctgatcgagg gaggccctct ggtggtgctc 1021 ctggacgtgg gtgcctcagc ttgggccctg gagcgctgga agggagagct ctgggatacc 1081 tgcaacatcg gagtgccgtg gtacgaccgc gaggccaacg acgctgtcct ctttggcttt 1141 ctgaccacct ggttcctggg tgagttcctg gcacagagtg aggagaagcc acatgtggtt 1201 gctcacttcc atgagtggtt ggcaggcgtt ggactctgcc tgtgtcgtgc ccggcgactg 1261 cctgtagcaa ccatcttcac cacccatgcc acgctgctgg ggcgctacct gtgtgccggt 1321 gccgtggact tctacaacaa cctggagaac ttcaacgtgg acaaggaagc aggggagagg 1381 cagatctacc accgatactg catggaaagg gcggcagccc actgcgctca cgtcttcact 1441 actgtgtccc agatcaccgc catcgaggca cagcacttgc tcaagaggaa accagatatt 1501 gtgaccccca atgggctgaa tgtgaagaag ttttctgcca tgcatgagtt ccagaacctc 1561 catgctcaga gcaaggctcg aatccaggag tttgtgcggg gccattttta tgggcatctg 1621 gacttcaact tggacaagac cttatacttc tttatcgccg gccgctatga gttctccaac 1681 aagggtgctg acgtcttcct ggaggcattg gctcggctca actatctgct cagagtgaac 1741 ggcagcgagc agacagtggt tgccttcttc atcatgccag cgcggaccaa caatttcaac 1801 gtggaaaccc tcaaaggcca agctgtgcgc aaacagcttt gggacacggc caacacggtg 1861 aaggaaaagt tcgggaggaa gctttatgaa tccttactgg ttgggagcct tcccgacatg 1921 aacaagatgc tggataagga agacttcact atgatgaaga gagccatctt tgcaacgcag 1981 cggcagtctt tcccccctgt gtgcacccac aatatgctgg atgactcctc agaccccatc 2041 ctgaccacca tccgccgaat cggcctcttc aatagcagtg ccgacagggt gaaggtgatt 2101 ttccacccgg agttcctctc ctccacaagc cccctgctcc ctgtggacta tgaggagttt 2161 gtccgtggct gtcaccttgg agtcttcccc tcctactatg agccttgggg ctacacaccg 2221 gctgagtgca cggttatggg aatccccagt atctccacca atctctccgg cttcggctgc 2281 ttcatggagg aacacatcgc agacccctca gcttacggta tctacattct tgaccggcgg 2341 ttccgcagcc tggatgattc ctgctcgcag ctcacctcct tcctctacag tttctgtcag 2401 cagagccggc ggcagcgtat catccagcgg aaccgcacgg agcgcctctc cgaccttctg 2461 gactggaaat acctaggccg gtactatatg tctgcgcgcc acatggcgct gtccaaggcc 2521 tttccagagc acttcaccta cgagcccaac gaggcggatg cggcccaggg gtaccgctac 2581 ccacggccag cctcggtgcc accgtcgccc tcgctgtcac gacactccag cccgcaccag 2641 agtgaggacg aggaggatcc ccggaacggg ccgctggagg aagacggcga gcgctacgat 2701 gaggacgagg aggccgccaa ggaccggcgc aacatccgtg caccagagtg gccgcgccga 2761 gcgtcctgca cctcctccac cagcggcagc aagcgcaact ctgtggacac ggccacctcc 2821 agctcactca gcaccccgag cgagcccctc agccccacca gctccctggg cgaggagcgt 2881 aacgaaaact tgtactttca aggctCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2941 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 3001 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 3061 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 3121 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 3181 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 3241 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 3301 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 3361 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 3421 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 3481 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3541 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3601 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3661 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3721 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3781 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3841 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3901 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3961 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 4021 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 4081 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4141 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4201 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4261 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4321 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4381 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4441 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4501 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4561 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4621 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4681 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4741 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //