LOCUS GYS1-pGEc2-DEST 7432 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6910..7039) /note="3'-ITR" misc_feature complement(6608..6822) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 6060..6601 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5239..5261 /note="attB2" misc_feature 6039..6044 /note="BstB1 site" misc_feature 5262..5957 /note="Super GFP" misc_feature 5958..6008 /note="AviTag" misc_feature 6009..6032 /note="His Tag" PCR_frame 3002..5239 /note="Reading Frame" /set="Set 1" source 3006..5216 /dnas_title="GYS1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2986 IMAGE:3143019" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..5216 /dnas_title="GYS1" /gene="GYS1" /gene_synonym="GSY" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" CDS 3006..5216 /dnas_title="glycogen synthase 1 (muscle)" /gene="GYS1" /gene_synonym="GSY" /codon_start=1 /product="glycogen synthase 1 (muscle)" /protein_id="AAH02617.1" /db_xref="GI:12803569" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" /translation="MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIY TVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVY FGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAV DFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDI VTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEF SNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDT ANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSY YEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQ LTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYE PNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAA KDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN" misc_feature 3006..5216 /note="GYS1 coding region" misc_feature 5217..5237 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..5246) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..5246) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(5247..7432,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..5246 /source="GYS1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgcc tttaaaccgc actttgtcca tgtcctcact gccaggactg gaggactggg 3061 aggatgaatt cgacctggag aacgcagtgc tcttcgaagt ggcctgggag gtggctaaca 3121 aggtgggtgg catctacacg gtgctgcaga cgaaggcgaa ggtgacaggg gacgaatggg 3181 gcgacaacta cttcctggtg gggccgtaca cggagcaggg cgtgaggacc caggtggaac 3241 tgctggaggc ccccaccccg gccctgaaga ggacactgga ttccatgaac agcaagggct 3301 gcaaggtgta tttcgggcgc tggctgatcg agggaggccc tctggtggtg ctcctggacg 3361 tgggtgcctc agcttgggcc ctggagcgct ggaagggaga gctctgggat acctgcaaca 3421 tcggagtgcc gtggtacgac cgcgaggcca acgacgctgt cctctttggc tttctgacca 3481 cctggttcct gggtgagttc ctggcacaga gtgaggagaa gccacatgtg gttgctcact 3541 tccatgagtg gttggcaggc gttggactct gcctgtgtcg tgcccggcga ctgcctgtag 3601 caaccatctt caccacccat gccacgctgc tggggcgcta cctgtgtgcc ggtgccgtgg 3661 acttctacaa caacctggag aacttcaacg tggacaagga agcaggggag aggcagatct 3721 accaccgata ctgcatggaa agggcggcag cccactgcgc tcacgtcttc actactgtgt 3781 cccagatcac cgccatcgag gcacagcact tgctcaagag gaaaccagat attgtgaccc 3841 ccaatgggct gaatgtgaag aagttttctg ccatgcatga gttccagaac ctccatgctc 3901 agagcaaggc tcgaatccag gagtttgtgc ggggccattt ttatgggcat ctggacttca 3961 acttggacaa gaccttatac ttctttatcg ccggccgcta tgagttctcc aacaagggtg 4021 ctgacgtctt cctggaggca ttggctcggc tcaactatct gctcagagtg aacggcagcg 4081 agcagacagt ggttgccttc ttcatcatgc cagcgcggac caacaatttc aacgtggaaa 4141 ccctcaaagg ccaagctgtg cgcaaacagc tttgggacac ggccaacacg gtgaaggaaa 4201 agttcgggag gaagctttat gaatccttac tggttgggag ccttcccgac atgaacaaga 4261 tgctggataa ggaagacttc actatgatga agagagccat ctttgcaacg cagcggcagt 4321 ctttcccccc tgtgtgcacc cacaatatgc tggatgactc ctcagacccc atcctgacca 4381 ccatccgccg aatcggcctc ttcaatagca gtgccgacag ggtgaaggtg attttccacc 4441 cggagttcct ctcctccaca agccccctgc tccctgtgga ctatgaggag tttgtccgtg 4501 gctgtcacct tggagtcttc ccctcctact atgagccttg gggctacaca ccggctgagt 4561 gcacggttat gggaatcccc agtatctcca ccaatctctc cggcttcggc tgcttcatgg 4621 aggaacacat cgcagacccc tcagcttacg gtatctacat tcttgaccgg cggttccgca 4681 gcctggatga ttcctgctcg cagctcacct ccttcctcta cagtttctgt cagcagagcc 4741 ggcggcagcg tatcatccag cggaaccgca cggagcgcct ctccgacctt ctggactgga 4801 aatacctagg ccggtactat atgtctgcgc gccacatggc gctgtccaag gcctttccag 4861 agcacttcac ctacgagccc aacgaggcgg atgcggccca ggggtaccgc tacccacggc 4921 cagcctcggt gccaccgtcg ccctcgctgt cacgacactc cagcccgcac cagagtgagg 4981 acgaggagga tccccggaac gggccgctgg aggaagacgg cgagcgctac gatgaggacg 5041 aggaggccgc caaggaccgg cgcaacatcc gtgcaccaga gtggccgcgc cgagcgtcct 5101 gcacctcctc caccagcggc agcaagcgca actctgtgga cacggccacc tccagctcac 5161 tcagcacccc gagcgagccc ctcagcccca ccagctccct gggcgaggag cgtaacgaaa 5221 acttgtactt tcaaggctCC AGCTTTCTTG TACAAAGTGG TATGAGCAAG GGCGAGGAAC 5281 TGTTCACCGG CGTGGTGCCC ATCCTGGTGG AACTGGATGG CGACGTGAAC GGCCACAAGT 5341 TCTCTGTGCG GGGAGAGGGC GAAGGCGACG CCACAAATGG CAAGCTGACC CTGAAGTTCA 5401 TCTGCACCAC CGGCAAGCTG CCCGTGCCTT GGCCTACCCT CGTGACCACA CTGACCTACG 5461 GCGTGCAGTG CTTCAGCAGA TACCCCGACC ATATGAAGCG GCACGACTTC TTCAAGAGCG 5521 CCATGCCCGA GGGCTACGTG CAGGAACGGA CCATCAGCTT CAAGGACGAC GGCACCTACA 5581 AGACCAGAGC CGAAGTGAAG TTCGAGGGCG ACACCCTCGT GAACCGGATC GAGCTGAAGG 5641 GCATCGATTT CAAAGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AACTTCAACA 5701 GCCACAACGT GTACATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA 5761 TCCGGCACAA CGTGGAAGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG CAGAACACCC 5821 CCATCGGAGA TGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGAGCGTGC 5881 TGAGCAAGGA CCCCAACGAG AAGCGGGACC ACATGGTGCT GCTGGAATTC GTGACCGCCG 5941 CTGGCATCAC CCACGGCATG AGCGGCCTGA ACGACATCTT CGAGGCCCAG AAAATCGAGT 6001 GGCACGAGCA CCACCATCAT CACCATCACC ACTGATGATT CGAAAAATAA TAAGGATCCA 6061 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 6121 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 6181 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 6241 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 6301 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 6361 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 6421 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 6481 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 6541 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 6601 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 6661 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 6721 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 6781 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 6841 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 6901 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 6961 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 7021 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 7081 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 7141 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 7201 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 7261 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 7321 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 7381 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //