LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2909..3005) /label=attL2 /note="attL2 (c)" misc_signal complement(3020..3039) /note="T7 Promoter/priming site (c)" primer_bind 3047..3063 /note="M13 Reverse priming site" gene 3176..3985 /note="Kanamycin resistance gene" rep_origin 4106..4779 /note="pUC origin" vector join(2917..4782,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2902 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2986 IMAGE:3143019" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2902 /gene="GYS1" /gene_synonym=GSY /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" CDS 692..2902 /dnas_title="glycogen synthase 1 (muscle)" /gene="GYS1" /gene_synonym=GSY /codon_start=1 /product="glycogen synthase 1 (muscle)" /protein_id="AAH02617.1" /db_xref="GI:12803569" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" /translation="MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIY TVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVY FGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAV DFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDI VTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEF SNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDT ANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSY YEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQ LTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYE PNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAA KDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN" misc_feature 692..2902 /note="GYS1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catgccttta aaccgcactt tgtccatgtc 721 ctcactgcca ggactggagg actgggagga tgaattcgac ctggagaacg cagtgctctt 781 cgaagtggcc tgggaggtgg ctaacaaggt gggtggcatc tacacggtgc tgcagacgaa 841 ggcgaaggtg acaggggacg aatggggcga caactacttc ctggtggggc cgtacacgga 901 gcagggcgtg aggacccagg tggaactgct ggaggccccc accccggccc tgaagaggac 961 actggattcc atgaacagca agggctgcaa ggtgtatttc gggcgctggc tgatcgaggg 1021 aggccctctg gtggtgctcc tggacgtggg tgcctcagct tgggccctgg agcgctggaa 1081 gggagagctc tgggatacct gcaacatcgg agtgccgtgg tacgaccgcg aggccaacga 1141 cgctgtcctc tttggctttc tgaccacctg gttcctgggt gagttcctgg cacagagtga 1201 ggagaagcca catgtggttg ctcacttcca tgagtggttg gcaggcgttg gactctgcct 1261 gtgtcgtgcc cggcgactgc ctgtagcaac catcttcacc acccatgcca cgctgctggg 1321 gcgctacctg tgtgccggtg ccgtggactt ctacaacaac ctggagaact tcaacgtgga 1381 caaggaagca ggggagaggc agatctacca ccgatactgc atggaaaggg cggcagccca 1441 ctgcgctcac gtcttcacta ctgtgtccca gatcaccgcc atcgaggcac agcacttgct 1501 caagaggaaa ccagatattg tgacccccaa tgggctgaat gtgaagaagt tttctgccat 1561 gcatgagttc cagaacctcc atgctcagag caaggctcga atccaggagt ttgtgcgggg 1621 ccatttttat gggcatctgg acttcaactt ggacaagacc ttatacttct ttatcgccgg 1681 ccgctatgag ttctccaaca agggtgctga cgtcttcctg gaggcattgg ctcggctcaa 1741 ctatctgctc agagtgaacg gcagcgagca gacagtggtt gccttcttca tcatgccagc 1801 gcggaccaac aatttcaacg tggaaaccct caaaggccaa gctgtgcgca aacagctttg 1861 ggacacggcc aacacggtga aggaaaagtt cgggaggaag ctttatgaat ccttactggt 1921 tgggagcctt cccgacatga acaagatgct ggataaggaa gacttcacta tgatgaagag 1981 agccatcttt gcaacgcagc ggcagtcttt cccccctgtg tgcacccaca atatgctgga 2041 tgactcctca gaccccatcc tgaccaccat ccgccgaatc ggcctcttca atagcagtgc 2101 cgacagggtg aaggtgattt tccacccgga gttcctctcc tccacaagcc ccctgctccc 2161 tgtggactat gaggagtttg tccgtggctg tcaccttgga gtcttcccct cctactatga 2221 gccttggggc tacacaccgg ctgagtgcac ggttatggga atccccagta tctccaccaa 2281 tctctccggc ttcggctgct tcatggagga acacatcgca gacccctcag cttacggtat 2341 ctacattctt gaccggcggt tccgcagcct ggatgattcc tgctcgcagc tcacctcctt 2401 cctctacagt ttctgtcagc agagccggcg gcagcgtatc atccagcgga accgcacgga 2461 gcgcctctcc gaccttctgg actggaaata cctaggccgg tactatatgt ctgcgcgcca 2521 catggcgctg tccaaggcct ttccagagca cttcacctac gagcccaacg aggcggatgc 2581 ggcccagggg taccgctacc cacggccagc ctcggtgcca ccgtcgccct cgctgtcacg 2641 acactccagc ccgcaccaga gtgaggacga ggaggatccc cggaacgggc cgctggagga 2701 agacggcgag cgctacgatg aggacgagga ggccgccaag gaccggcgca acatccgtgc 2761 accagagtgg ccgcgccgag cgtcctgcac ctcctccacc agcggcagca agcgcaactc 2821 tgtggacacg gccacctcca gctcactcag caccccgagc gagcccctca gccccaccag 2881 ctccctgggc gaggagcgta acTAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2941 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 3001 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 3061 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 3121 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 3181 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 3241 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 3301 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 3361 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 3421 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 3481 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 3541 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 3601 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 3661 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 3721 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 3781 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 3841 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3901 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3961 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 4021 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 4081 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 4141 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 4201 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 4261 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 4321 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 4381 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 4441 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4501 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4561 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4621 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4681 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4741 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //