LOCUS GYS1-pGEn1-DEST 6793 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5421..5962 /note="WPRE" misc_recomb 5383..5405 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5969..6183) /note="BGH pA" misc_feature complement(6271..6400) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5376 /dnas_title="GYS1-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2986 IMAGE:3143019" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..5376 /dnas_title="GYS1" /gene="GYS1" /gene_synonym="GSY" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" CDS 3166..5376 /dnas_title="glycogen synthase 1 (muscle)" /gene="GYS1" /gene_synonym="GSY" /codon_start=1 /product="glycogen synthase 1 (muscle)" /protein_id="AAH02617.1" /db_xref="GI:12803569" /db_xref="GeneID:2997" /db_xref="HGNC:4706" /db_xref="MIM:138570" /translation="MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIY TVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVY FGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAV DFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDI VTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEF SNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDT ANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSY YEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQ LTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYE PNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAA KDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN" misc_feature 3166..5376 /note="GYS1 coding region" vector join(5391..6793,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5390 /source="GYS1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcatgcc tttaaaccgc 3181 actttgtcca tgtcctcact gccaggactg gaggactggg aggatgaatt cgacctggag 3241 aacgcagtgc tcttcgaagt ggcctgggag gtggctaaca aggtgggtgg catctacacg 3301 gtgctgcaga cgaaggcgaa ggtgacaggg gacgaatggg gcgacaacta cttcctggtg 3361 gggccgtaca cggagcaggg cgtgaggacc caggtggaac tgctggaggc ccccaccccg 3421 gccctgaaga ggacactgga ttccatgaac agcaagggct gcaaggtgta tttcgggcgc 3481 tggctgatcg agggaggccc tctggtggtg ctcctggacg tgggtgcctc agcttgggcc 3541 ctggagcgct ggaagggaga gctctgggat acctgcaaca tcggagtgcc gtggtacgac 3601 cgcgaggcca acgacgctgt cctctttggc tttctgacca cctggttcct gggtgagttc 3661 ctggcacaga gtgaggagaa gccacatgtg gttgctcact tccatgagtg gttggcaggc 3721 gttggactct gcctgtgtcg tgcccggcga ctgcctgtag caaccatctt caccacccat 3781 gccacgctgc tggggcgcta cctgtgtgcc ggtgccgtgg acttctacaa caacctggag 3841 aacttcaacg tggacaagga agcaggggag aggcagatct accaccgata ctgcatggaa 3901 agggcggcag cccactgcgc tcacgtcttc actactgtgt cccagatcac cgccatcgag 3961 gcacagcact tgctcaagag gaaaccagat attgtgaccc ccaatgggct gaatgtgaag 4021 aagttttctg ccatgcatga gttccagaac ctccatgctc agagcaaggc tcgaatccag 4081 gagtttgtgc ggggccattt ttatgggcat ctggacttca acttggacaa gaccttatac 4141 ttctttatcg ccggccgcta tgagttctcc aacaagggtg ctgacgtctt cctggaggca 4201 ttggctcggc tcaactatct gctcagagtg aacggcagcg agcagacagt ggttgccttc 4261 ttcatcatgc cagcgcggac caacaatttc aacgtggaaa ccctcaaagg ccaagctgtg 4321 cgcaaacagc tttgggacac ggccaacacg gtgaaggaaa agttcgggag gaagctttat 4381 gaatccttac tggttgggag ccttcccgac atgaacaaga tgctggataa ggaagacttc 4441 actatgatga agagagccat ctttgcaacg cagcggcagt ctttcccccc tgtgtgcacc 4501 cacaatatgc tggatgactc ctcagacccc atcctgacca ccatccgccg aatcggcctc 4561 ttcaatagca gtgccgacag ggtgaaggtg attttccacc cggagttcct ctcctccaca 4621 agccccctgc tccctgtgga ctatgaggag tttgtccgtg gctgtcacct tggagtcttc 4681 ccctcctact atgagccttg gggctacaca ccggctgagt gcacggttat gggaatcccc 4741 agtatctcca ccaatctctc cggcttcggc tgcttcatgg aggaacacat cgcagacccc 4801 tcagcttacg gtatctacat tcttgaccgg cggttccgca gcctggatga ttcctgctcg 4861 cagctcacct ccttcctcta cagtttctgt cagcagagcc ggcggcagcg tatcatccag 4921 cggaaccgca cggagcgcct ctccgacctt ctggactgga aatacctagg ccggtactat 4981 atgtctgcgc gccacatggc gctgtccaag gcctttccag agcacttcac ctacgagccc 5041 aacgaggcgg atgcggccca ggggtaccgc tacccacggc cagcctcggt gccaccgtcg 5101 ccctcgctgt cacgacactc cagcccgcac cagagtgagg acgaggagga tccccggaac 5161 gggccgctgg aggaagacgg cgagcgctac gatgaggacg aggaggccgc caaggaccgg 5221 cgcaacatcc gtgcaccaga gtggccgcgc cgagcgtcct gcacctcctc caccagcggc 5281 agcaagcgca actctgtgga cacggccacc tccagctcac tcagcacccc gagcgagccc 5341 ctcagcccca ccagctccct gggcgaggag cgtaacTAGG ACCCAGCTTT CTTGTACAAA 5401 GTGGTTGATA GCTTGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 5461 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 5521 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 5581 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5641 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5701 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5761 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5821 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5881 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5941 TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 6001 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 6061 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 6121 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 6181 GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA 6241 AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC 6301 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 6361 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG 6421 CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC 6481 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 6541 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 6601 AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC 6661 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 6721 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 6781 GCTTACAATT TAG //