LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2807..2903) /label=attL2 /note="attL2 (c)" misc_signal complement(2918..2937) /note="T7 Promoter/priming site (c)" primer_bind 2945..2961 /note="M13 Reverse priming site" gene 3074..3883 /note="Kanamycin resistance gene" rep_origin 4004..4677 /note="pUC origin" vector join(2815..4680,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2800 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161588 IMAGE:8992026" /tissue_type="Pooled, cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus, PCR rescued clones" /clone_lib="NIH_MGC_362" /lab_host="DH10B" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AATGATCC" gene 692..2800 /gene="GYS2" /db_xref="GeneID:2998" /db_xref="HGNC:4707" /db_xref="MIM:138571" CDS 692..2800 /dnas_title="glycogen synthase 2 (liver)" /gene="GYS2" /codon_start=1 /product="glycogen synthase 2 (liver)" /protein_id="AAI26311.1" /db_xref="GI:116496869" /db_xref="GeneID:2998" /db_xref="HGNC:4707" /db_xref="MIM:138571" /translation="MLRGRSLSVTSLGGLPQWEVEELPVEELLLFEVAWEVTNKVGGI YTVIQTKAKTTADEWGENYFLIGPYFEHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQV HFGRWLIEGSPYVVLFDIGYSAWNLDRWKGDLWEACSVGIPYHDREANDMLIFGSLTA WFLKEVTDHADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLGRYLCAANI DFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDV VTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFYGHLDFDLEKTLFLFIAGRYEF SNKGADIFLESLSRLNFLLRMHKSDITVVVFFIMPAKTNNFNVETLKGQAVRKQLWDV AHSVKEKFGKKLYDALLRGEIPDLNDILDRDDLTIMKRAIFSTQRQSLPPVTTHNMID DSTDPILSTIRRIGLFNNRTDRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSY YEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSPDDSCNQ LTKFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPDKFHVE LTSPPTTEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEVEDERYDEEEEAERDRLNIK SPFSLSHVPHGKKKLHGEYKN" misc_feature 692..2800 /note="GYS2 coding region" misc_difference 1778..1778 /gene="GYS2" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catgcttcga ggccgatccc tctctgtaac 721 atccctgggt gggcttcccc agtgggaagt cgaagaactt cctgtggagg agttactgct 781 ctttgaagtt gcttgggaag tgaccaataa agttggaggc atctatactg tgattcagac 841 aaaggccaaa acaacagcag atgaatgggg agagaactat tttctgatag gtccatattt 901 tgagcataat atgaagactc aggtggaaca gtgtgaacct gtaaatgatg ctgtcagaag 961 agcagtggac gcaatgaata agcatggctg ccaggtgcat tttggaagat ggctgataga 1021 aggaagtcct tatgtggtac tttttgacat aggctattca gcttggaatc tggacaggtg 1081 gaagggtgac ctctgggaag catgcagtgt cggcattcct tatcatgacc gagaagccaa 1141 tgatatgctg atatttggat ctttaactgc ctggttctta aaagaggtga cagatcatgc 1201 agatggtaaa tatgtcgttg cccaattcca tgaatggcag gctggaattg gactgatcct 1261 ttctcgagcc aggaaacttc ctattgccac aatatttaca acccacgcta cactacttgg 1321 gaggtatctc tgtgcagcaa atattgattt ctacaaccat cttgataagt ttaacattga 1381 caaagaggct ggggaaaggc agatttacca ccggtactgc atggagcgag cttccgttca 1441 ttgcgctcac gtgttcacca cggtttctga aataacagca atagaagctg aacatatgct 1501 gaagagaaag cctgatgtag ttactccaaa cggcttgaat gttaagaaat tttcagcagt 1561 gcatgagttt caaaatctac atgccatgta caaggccaga atccaagatt ttgttcgagg 1621 tcatttctat ggtcatctcg actttgatct tgaaaagact ttgttccttt tcattgctgg 1681 gaggtatgag ttttcaaaca aaggagctga catcttccta gaatccttat ccaggctaaa 1741 tttcctgctg aggatgcata aaagtgacat cacagtggtg gtgtttttca ttatgcctgc 1801 caagacaaat aatttcaacg tggaaaccct gaaaggacaa gcagtgcgaa aacagctgtg 1861 ggatgttgca cattctgtga aggaaaagtt tggaaaaaaa ctctatgatg cattattaag 1921 aggagaaatt cctgacctga acgatatttt agatcgagat gatctaacaa ttatgaaaag 1981 agccatcttt tcaactcagc gacagtcatt gcccccagtg accacgcaca acatgattga 2041 tgactccacc gaccccatcc tcagcaccat tagacggatt ggacttttca acaaccgcac 2101 agatagagtc aaggtgattt tgcacccaga gtttctatcc tccaccagtc ccttactacc 2161 catggactat gaagagtttg ttagaggttg tcatcttgga gtatttccat catactatga 2221 accctggggt tatactccag ctgaatgcac tgtgatgggt atccccagtg tgaccacgaa 2281 tctctccggg tttggctgtt tcatgcagga gcacgtggct gatcctactg cttacggtat 2341 ttacatcgtt gacaggcggt tccgttctcc agatgattct tgcaatcagc tgactaagtt 2401 tctctatgga ttttgcaaac agtcacgccg ccaaaggatt atccagagga acagaactga 2461 gaggctctca gatcttctgg attggagata cttaggcaga tattaccagc atgccagaca 2521 cctgacatta agcagagctt ttccagataa attccatgtg gaactaacat caccaccaac 2581 gacagaagga tttaaatatc ccaggccttc ctcagtacca ccttctcctt cagggtctca 2641 ggcctccagt cctcagagca gtgatgtgga agatgaagtg gaggatgaga gatacgatga 2701 ggaagaggag gctgaaaggg atcggttaaa tatcaagtca ccattttcac tgagccacgt 2761 tcctcatggg aagaaaaagc tgcatggtga atataagaac TAGGACCCAG CTTTCTTGTA 2821 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2881 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2941 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 3001 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 3061 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 3121 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 3181 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 3241 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3301 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3361 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3421 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3481 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3541 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3601 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3661 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3721 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3781 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3841 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3901 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3961 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 4021 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 4081 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 4141 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 4201 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 4261 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4321 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4381 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4441 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4501 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4561 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4621 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //