LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2171..2267) /label=attL2 /note="attL2 (c)" misc_signal complement(2282..2301) /note="T7 Promoter/priming site (c)" primer_bind 2309..2325 /note="M13 Reverse priming site" gene 2438..3247 /note="Kanamycin resistance gene" rep_origin 3368..4041 /note="pUC origin" PCR_frame 669..2171 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2191) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2188) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2179..4044,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2149..2169 /note="TEV site" source 673..2148 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:167122 IMAGE:8860455" /tissue_type="Clones donated by RIKEN" /clone_lib="NIH_MGC_424" /lab_host="DH10B" /note="Vector: pFLC1" gene 673..2148 /gene="ALG11" /gene_synonym=GT8 /gene_synonym=UTP14C /db_xref="GeneID:440138" /db_xref="HGNC:32456" CDS 673..2148 /dnas_title="asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast)" /gene="ALG11" /gene_synonym=GT8 /gene_synonym=UTP14C /codon_start=1 /product="asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast)" /protein_id="AAI42999.1" /db_xref="GI:148745765" /db_xref="GeneID:440138" /db_xref="HGNC:32456" /translation="MAAGERSWCLCKLLRFFYSLFFPGLIVCGTLCVCLVIVLWGIRL LLQRKKKLVSTSKNGKNQMVIAFFHPYCNAGGGGERVLWCALRALQKKYPEVVYVVYT GDVNVNGQQILEGAFRRFNIRLIHPVQFVFLRKRYLVEDSLYPHFTLLGQSLGSIFLG WEALMQCVPDVYIDSMGYAFTLPLFKYIGGCQVGSYVHYPTISTDMLSVVKNQNIGFN NAAFITRNPFLSKVKLIYYYLFAFIYGLVGSCSDVVMVNSSWTLNHILSLWKVGNCTN IVYPPCDVQTFLDIPLHEKKMTPGHLLVSVGQFRPEKNHPLQIRAFAKLLNKKMVESP PSLKLVLIGGCRNKDDELRVNQLRRLSEDLGVQEYVEFKINIPFDELKNYLSEATIGL HTMWNEHFGIGVVECMAAGTIILAHNSGGPKLDIVVPHEGDITGFLAESEEDYAETIA HILSMSAEKRLQIRKSARASVSRFSDQEFEVTFLSSVEKLFK" misc_difference 959..959 /gene="ALG11" /gene_synonym=GT8 /gene_synonym=UTP14C /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2148 /note="ALG11 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcggc cggcgaaagg agctggtgcc tgtgcaagtt gttgaggttt 721 ttttattcat tattcttccc tgggctcatt gtatgtggaa ctttatgtgt gtgtttggtc 781 attgtccttt ggggaatcag actgctgcta cagagaaaga aaaaattagt gtcaactagc 841 aaaaatggga aaaatcaaat ggtgattgca ttttttcatc catactgcaa tgctggtgga 901 ggaggagaaa gagttttatg gtgtgcttta agagccctgc agaaaaagta tcctgaagta 961 gtttatgttg tttataccgg cgatgttaat gtcaacggtc aacagatact agaaggtgct 1021 ttcagaagat ttaacatcag attaattcac ccagtgcagt ttgttttttt aaggaaacgc 1081 tatcttgtgg aagattcact gtatcctcac ttcacactgc tgggccaaag tctaggatcc 1141 atttttcttg gctgggaagc tctaatgcag tgtgttcctg atgtttacat tgattcaatg 1201 ggatacgctt ttacgcttcc tctgtttaag tatatagggg gttgccaagt tggaagctat 1261 gttcattatc ctactatcag caccgacatg ctctctgtag tgaagaatca aaatattgga 1321 tttaataatg cagccttcat taccaggaat ccttttctca gcaaagtaaa gctcatctac 1381 tactatttat ttgcttttat ttatggactt gttggttctt gcagtgatgt agtcatggtc 1441 aattcttctt ggacactaaa ccatattctc tcactatgga aagttgggaa ttgcactaac 1501 attgtttatc caccttgtga tgtgcagaca tttctggaca ttcccttaca tgagaaaaag 1561 atgaccccag gacatttgct ggtttctgtt ggccagttta ggccggaaaa gaatcatcca 1621 ttgcagatca gagcctttgc taaattgctg aataagaaga tggttgagtc acctccttcg 1681 cttaaacttg tcctcattgg aggttgtcgt aacaaagatg atgaacttag ggtaaaccaa 1741 ctgagaaggc tgtctgagga tttaggagtt caagaatatg tggaatttaa aataaacatt 1801 ccatttgatg aattaaagaa ttatttgtct gaagcaacaa ttggtctgca taccatgtgg 1861 aacgagcatt ttgggattgg agttgtggag tgtatggcag ctggcacaat tatccttgca 1921 cacaattcgg ggggcccaaa gcttgacatt gtggttcctc acgaaggaga tataactggc 1981 tttctggctg agagtgaaga agactatgct gaaactatcg ctcacattct ttccatgtct 2041 gcagaaaaga gactccaaat cagaaaaagt gctcgtgcat ctgtaagcag attctctgat 2101 caggaatttg aagtgacatt cctatcatct gtggaaaagt tatttaagga aaacttgtac 2161 tttcaaggct CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 2221 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA 2281 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT 2341 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA 2401 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC 2461 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG 2521 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC 2581 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT 2641 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC 2701 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT 2761 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG 2821 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC 2881 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG 2941 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC 3001 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 3061 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC 3121 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA 3181 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT 3241 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG 3301 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC 3361 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3421 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3481 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3541 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3601 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3661 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3721 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 3781 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 3841 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 3901 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 3961 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4021 TTGCTGGCCT TTTGCTCACA TGTT //